Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"translation inhibition"”.
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Mechanism of transmissible gastroenteritis virus nsp1-mediated translation inhibition
… of mRNAs. Nsp1 of TGEV strongly inhibits the translation of capped mRNAs, as well as certain internal ribosome entry site (IRES)- containing mRNAs, in HeLa S10 extract but not in rabbit reticulocyte lysate or wheat germ extract. In this dissertation the mechanism of TGEV nsp1-mediated …
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Rapid turnover of McL-1 couples translation to cell survival and apoptosis
Global inhibition of translation contributes to the induction of apoptosis by various cell stimuli including inhibition of PI 3-kinase/Akt survival signaling, viral infection, lipopolysaccharide, and tumor necrosis factor alpha. However, the mechanism by which translation inhibition promotes …
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Inhibition of Mitochondrial Translation as a Therapeutic Strategy for Acute Myeloid Leukemia
Inhibition of mitochondrial translation as a therapeutic strategy for acute myeloid leukemia Marko Škrtić Doctor of Philosophy Institute of Medical Science University of Toronto 2012 Abstract Intro: Acute myeloid leukemia (AML) therapies have remained unchanged for 20 years, and thus new therapies …
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Investigating the translational and metabolic reprogramming of the bone marrow niche in myeloid malignancy
… cells surviving chemotherapy rely on high mRNA translation and their microenvironmental metabolic support to drive relapse. However, the role of translational reprogramming in the niche was, until now, unclear. This study found that relapsing AML cells increase translation in their bone marrow …
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On the expression, function and regulation of the murine Ifit family of antiviral RNA-binding proteins.
… viral infection. In humans, IFIT1 inhibits translation at the initiation stage by binding directly to the 5' terminus of foreign RNA, precluding the recruitment of the cap-binding translation initiation factor complex eIF4F. IFIT1 is highly specific for ‘non-self’ cap0 RNA, which lacks …
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Expression strategies for plant-based production of a vaccine adjuvant
… ricin was shown using a protein translation inhibition assay. Expression of ricin was also confirmed using transient transformation of hairy root cultures. Future experiments will address the practical use of the tobacco-synthesized ricin as adjuvant, as well as the expression of …
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mRNA localization in Caenorhabditis elegans embryogenesis
… can regulate myriad processes as varied as co-translationally organizing protein components for complex assembly (Budding yeast translation factor mRNA granules), reinforcing translation inhibition (Germ granules) or activation (Neuronal granules), and facilitating the organization of other …
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Characterization of KPAP, a Novel Pokeweed Antiviral Protein Isoform
… eukaryotes. This depurination results in protein translation inhibition leading to a concentration-dependent cytotoxic consequence. Many RIPs in the plant kingdom are being discovered with new genomic sequencing technologies but, many of them remain uncharacterized. Recently, our lab discovered a …
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Role of p53 and its isoforms in the expression of FGF-2 and tumoral neovascularization
… in a human osteosarcoma cell line U2OS, at translational, transcriptional and secretion levels. It resulted in a sophisticated mode of regulation mediated by a transient IRES-dependent translation inhibition of FGF-2. Our data reveal D133p53 isoforms as activators of angiogenesis and tumour …
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Dynamics of E. coli genome and cytosol under antibiotics
… gene expression measurements during sub-lethal translation inhibition. Specifically, we compare responses to tetracycline and chloramphenicol from constitutive and ribosomal promoters in Ori3 and Ter3 chromosomal positions over long (7 h) treatment times in exponentially growing bacteria. We …
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An Investigation of TorsinA Interaction Partners
… associated with Calnexin 3 hours following translation inhibition. This finding again deviates from the Calnexin substrate model, as Calnexin preferentially binds newly synthesized proteins. Therefore, we conclude that the interaction between TorsinA and Calnexin likely has functional …
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Pim Kinase Inhibition: Single Agent and Combination Approaches In B-Cell Lymphoma
… various substrates that regulate transcription, translation, cell cycle, and survival pathways in both hematological and solid tumors.</p> <p>Mantle cell lymphoma (MCL) is an aggressive B-cell lymphoma. Elevated Pim kinase levels are common in MCL, and it negatively correlates with patient …
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Trashing old notions: the significance of small tRNAs in Trichomonas vaginalis extracellular vesicles and implications for host-parasite communication
… highly abundant fragments contained a known translation inhibitory motif, the terminal oligoguanine (TOG). In the second chapter, quantitative PCR was employed to compare the abundance of selected fragments in cells and EVs of different sizes subjected to stress-free and traditional …
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Defining the Cellular and Molecular Basis of Spondyloepiphyseal dysplasia tarda
… activate signalling pathways that result in translation inhibition by sequestering free mRNAs and ribonucleoproteins in cytosolic aggregates named stress granules (SGs). I demonstrated that SGs control secretion by recruiting key components of ER-to- Golgi trafficking and thus inhibit …
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Impaired ketogenesis is implicated in intestinal stem cell function and epithelial regeneration in ulcerative colitis
… single cell energetic metabolism by profiling translation inhibition (SCENITH) on treatment-naïve UC patient epithelial samples and on UC patient derived organoids. Single-cell quantitative immunofluorescence showed a loss of mitochondrial oxidative phosphorylation proteins Complex I and IV, …
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Translational Regulation in the 5′UTR of Enteroviruses
… whether these represent alternative sites of translation initiation has not yet been determined. Since many enteroviruses have additional AUGs in the region of the dVI AUG, we evaluated its effects on translation in three frames in coxsackievirus A13 (CVA-13). CVA-13 naturally has an …
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Development of Computational Tools and Resources for Cotton microRNA Analysis
… levels. miRNAs target genes for mRNA cleavage or translation inhibition based on the complementary between the mRNAs and its corresponding miRNAs, and these miRNA target genes control development timing, organ development and response to environmental stress; thus miRNAs have been shown to play …
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Exploring SARM1 as a target to delay programmed axon degeneration
… degeneration) can occur after physical injury, inhibition of axon transport, exposure to neurotoxic compounds, and in diseases involving mitochondrial or metabolic dysfunction. There is increasing evidence that this pathway can be activated in human painful neuropathies and preclinical evidence …