Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 83 for “"transient expression"”.
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Developing and applying transient expression systems to identify and understand gene regulatory elements in plants
In this dissertation, I develop transient expression systems to enable massively parallel reporter assays (MPRAs) in crop models. I apply these transient assay systems to identify, characterize and design plant gene regulatory elements. To allow for the scale of MPRAs, I develop a high-efficiency …
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Construction of inducible expression systems for controlled transfer of Agrobacterium tumefaciens T-DNA in plant transient expression systems /
… facilities using Agrobacterium-mediated transient transfection of plants. However, transfection methods suitable for open field applications are still desirable as a cheaper alternative. Biosafety concerns related to the use of recombinant agrobacteria in an industrial transfection …
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Factors influencing transient gene expression in electroporated tall fescue (Festuca arundinacea Schreb.) protoplasts
… for tall fescue, several factors influencing transient gene expression were studied in protoplasts, after the reporter β-glucuronidase (GUS) gene was introduced by electroporation. In a time-course study of transient gene expression, GUS activity peaked at 24 h after electroporation. Among the …
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Developing Adeno-Associated Viral Vectors As A Gene Editing Platform
… their ability to generate sustained transgene expression. The potential for low or transient expression of AAV- delivered transgenes has been unexamined. This creates a blind spot for AAV-based genome editing applications because only minimal transgene expression is required for activity. To …
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Antibody technology identifies distinct mechanisms controlling the lengths of filopodia-like structures
… with different proteomic techniques and transient expression. The antibody panel was strikingly split into two groups: (1) Antibodies that lengthen FLS and do not recognise specific proteins in their eluates or (2) Antibodies that shorten FLS and show specific candidate proteins. …
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Control of major histocompatibility Class I antigen expression in adenovirus transformed cells.
… transcription of this gene. MHC class I expression is down-regulated in cells expressing Adenovirus (Ad) 12 E1A compared to cells expressing E1A from Ad2 or Ad5. The possible involvement of these sequences and the factors that bind to them in this down-regulation was investigated using …
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Car-Modified T Cells Capable of Distinguishing Normal Cells From Malignant Cells
… patients. EGFR also has widespread normal tissue expression. To target EGFR on glioblastoma while reducing the potential for normal tissue toxicity, EGFR-specific CAR generated from cetuximab, Cetux-CAR, was transiently expressed in T cells by RNA-modification. RNA-modified CAR<sup>+</sup> T cells …
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Development of a plant-made immunoassay for the detection of Porcine circovirus infections in South African swine herds
… their use in developing countries. Plant-based transient expression systems have recently been investigated to express PCV-2 CP for a cheaper diagnostic reagent. The aim of this study was to develop an inexpensive lateral flow device to be able to test for PCV infection in pig herds. Production …
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Decoding the Transcriptional Specificity of Auxin Signaling: A Synthetic Biology Approach
… receptors that work together to modulate gene expression. Functional diversity within these modular components, such as differences in DNA binding, protein-protein interactions, subcellular localization, and co-expression patterns is believed to act in concert to tune auxin signaling …
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The Use of Morphogenic Transcription Factors to Enhance Plant Regeneration
… (cells whose cell wall has been removed) for transient expression and subsequent regeneration of non-GMO, edited plants. However, efficient regeneration of plants from protoplasts is a bottleneck in the implementation of this technique. To create a universal method for protoplast regeneration, …
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Transient heterologous protein expression in green microalgae
… it is referred to as heterologous protein expression. The baculovirus insect cell culture, yeast, and plants, such as Nicotiana benthamiana, are only a few of the commercial expression systems where this technology has been extensively used. These systems, however, often have disadvantages …
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Characterization of a cold-responsive dehydrin promoter
… transformation system, while capable of transient expression of introduced constructs in protoplasts, was difficult for use for cold-inducible expression.
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Enhancing mRNA stability through the addition of stabilizing untranslated regions
… is introduced to cells or tissues to cause a transient expression of specific genes, has applications ranging from tissue engineering to neural regeneration. This transient nature of mRNA, however, limits the duration of gene upregulation, and potentially reduces the efficacy of mRNA therapy. …
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Development and Characterization of Viral-Based Gene Editing In Vivo
… because of their ability to achieve sustained expression from largely non-integrating episomal genomes. However, for genome editing applications, permanent expression of non-human proteins such as the bacterially-derived Cas9 nuclease is undesirable. Recent studies indicate a high prevalence of …
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A novel method for mammalian large genetic circuit assembly and delivery
… pieces. The assembled circuits can be used for transient expression and each individual circuit can be integrated into a cellular genome to create a stable cell line. Genetic circuits were constructed that contain several expression units, including inducible control units and fluorescent …
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Beak and feather disease virus candidate vaccine development
… in the inexpensive and highly scalable plant expression system, could satisfy these requirements as a potential subunit vaccine. In this work, truncated CP (ÄN40 CP) was first expressed in E. coli to successfully generate anti-CP polyclonal antibodies. ÄN40 CP and full-length CP transient …
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A functional study of the<em> Phytophthora infestans</em> Avr3a alleles and paralogs
… all Avr3a variants and PEX147-3 are stable upon transient expression in planta and in yeast cells.<br/><br/>In terms of host recognition, the protein products of the Avr3a alleles derived from Avr3aKI are recognised by the cognate host resistance gene product R3a whereas those derived from …
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The Immunogenicity of Plant-produced Human Papillomavirus (HPV) Virus-like particles (VLPs) in Mice
… costeffective alternative to conventionally used expression systems. The aim of this study was to evaluate the plant-based transient expression system as a tool to produce potentially cost-effective HPV L1 VLP-based vaccines, particularly for developing countries. Firstly, the L1 proteins of the 8 …
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Characterization of theWound-Induced Signaling Pathway In Tomato
… MPK1/2 and MPK3. Through the use of a protoplast transient expression assay, I have confirmed that MKK2 can phosphorylate MPK1/2 and MPK3, but MKK4 is not expressed in protoplasts. I have shown that negative regulation of MKK4 via conventional protein degradation mechanisms is not responsible for …
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Harnessing the manufacturing power of the plant system : production of recombinant human interleukin-2 in Nicotiana benthamiana
Various expression systems are employed for the mass production of recombinant proteins to be used for pharmaceutical, diagnostic and industrial purposes. In this thesis, I have explored the feasibility of producing recombinant human interleukin-2 (IL-2) in the green tissues of transgenic Nicotiana …
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