Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 42 for “"transgenic tobacco"”.
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Expression of Human Protein C in Transgenic Tobacco
… system for producing hPC on a large-scale basis. Tobacco (Nicotiana tabacum L.) is particularly well suited for use as a bioreactor for high-value recombinant proteins. Tobacco is one of the easiest plants to transform, it is an excellent biomass producer and can produce up to a million seeds from …
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Purification of an acidic recombinant protein from transgenic tobacco
Tobacco has been studied as a host for producing recombinant therapeutic proteins on a large-scale, commercial basis. However, the proteins expressed in tobacco usually need to be purified to high yield and purity from large amounts of biomass in order for their production to be commercially …
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Field trials to phenotype transgenic tobacco plants with improved RuBP regeneration
… were firstly to produce and identify homozygous transgenic tobacco plants and then to test these plants in field conditions for improved photosynthesis and yield. Transgenic Nicotiana tabacum plants were produced using gene constructs designed to either overexpress the native H-protein of the …
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Separation of Recombinant β-Glucuronidase from Transgenic Tobacco by Aqueous Two-Phase Extraction
… recombinant β-glucuronidase (rGUS), from transgenic tobacco with high yield and purity. Aqueous two-phase extraction (ATPE) is a powerful technique for separation and purification of proteins, and has the potential to replace an expensive chromatography step for the initial purification of …
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Pilot-scale process development for the purification of the recombinant antibody 2G12 from transgenic tobacco
… the industry reaches its production capacity. Transgenic plants offer an alternative production system to the established platforms, but this requires the development of downstream processing schemes tailored for both the platform and the product. The aim of this thesis, within the EU-funded …
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The cloning of calmodulincDNAs from barley and Arabidopsis and expression of barley calmodulin in transgenic tobacco
Calmodulin (CaM) is a calcium-binding protein found in eukaryotes, and appears to be essential for growth and development. CaM is thought to play a pivotal role in modulating biochemical pathways in conjunction with transient calcium fluxes into the cytoplasm. In order to better understand …
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Potential Impacts of Pharmaceutical Uses of Transgenic Tobacco: The Case of Human Serum Albumin and Gaucher's Disease Treatment
… and distribution of benefits from the use of transgenic tobacco as a production vehicle for pharmaceutical proteins. Ex-ante welfare benefits are estimated for the introduction of two biotech innovations. In both cases economic surplus model with imperfect competition is employed to assess the …
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The use of transgenic tobacco as a production and delivery system for a vaccine against hemorrhagic enteritis virus of turkeys
… antibodies against an HEV challenge. Using tobacco as a model, we sought to determine if a plant could be used to synthesize the HEV fiber protein and produce sufficient antigen to stimulate protective antibodies. To introduce the fiber gene into plants, the coding region of the HEV fiber …
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Engineering and characterization of single chain antibody fragments (scFvs) specific to key enzymes in polyamine biosynthesis and manipulation of polyamine pathway by constitutive expression of recombinant ODC and SDE enzymes in transgenic tobacco
… activities via heterologous gene expression in transgenic plants. Previous studies have indicated that the polyamine pathway is tightly controlled in the end product accumulation. The objective of this study was to achieve changes in the pathway by combining genetic engineering and antibody …
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Expression of recombinant porcine preprorelaxin in Nicotiana tabacum
… to express fully processed porcine relaxin in transgenic tobacco plants, as an alternative to current methods of producing relaxin. Two recombinant relaxin genes were constructed that contained the patatin signal peptide cDNA fused in frame to prorelaxin cDNA, which was codon-optimized for …
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Phytoextraction of heavy metal from contaminated soils using genetically modified plants
… that produce high levels of biomass using a transgenic approach is a promising strategy for the development of effective phytoremediation technologies (Krämer, et al. 2001). This thesis describes the use of recombinant Chinese hamster and Saccharomyces cerevisiae MTIIs for generating …
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Cloning and molecular characterization of vffl gene coding forisomes of vicia faba
… as well as tissue specificity was confirmed in transgenic tobacco plants using GUS as a reporter system. Sequence analysis revealed two conserved motifs shared with several previously reported phloem-specific promoters. Taken together, these results indicate that the 5'-upstream region of vff1 …
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Characterization of a potato Proteinase Inhibitor II gene whose expression in not wound-responsive
… gene. The chimeric genes were transferred to tobacco plants using Agrobacterium tumefaciens. The presence of the constructions in the transgenic tobacco plants was confirmed by polymerase chain reaction (PCR). Expression of CAT or GUS activities in transgenic plants driven by pin2T promoter …
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Tobacco Phospholipase D β1: Molecular Cloning and Biochemical Characterization
Transgenic tobacco plants were developed containing a partial PLD clone in antisense orientation. The PLD isoform targeted by the insertion was identified. A PLD clone was isolated from a cDNA library using the partial PLD as a probe: Nt10B1 shares 92% identity with PLDβ1 from tomato but lacks the …
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Studies to influence the carbon metabolism of the C 3 plant Nicotiana tabacum by expressing heterologous enzymes involved in C 4 -like CO 2 fixation
… and subsequent stepwise crosses, a series of transgenic tobacco plants were regenerated, in which all the enzymes required for the "C4-cycle" of the NADP-ME-type (PC/ME/DK or PS/PT) and PCK-type (PC/CK/PT) were introduced. As a consequence, biochemical and physiological changes occurred in …
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GENETIC MANIPULATION OF STORAGE ROOT DEVELOPMENT IN HORTICULTURAL CROPS
… development. This project centred on the use of transgenic methods to characterise the expression of the Bvcrkl promoter in order to assess its utility for the regulation of transgene expression, as well as providing more insight into the function of the Bvcrkl gene. Sugar beet is notoriously …
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Multi-omic profiling of NPQ improved nicotiana tabacum lines under uniform light conditions
… T-DNA cassette in Nicotiana tabacum L. (tobacco) has been shown to improve the rate of NPQ relaxation, resulting in yield increases under both greenhouse and field conditions (Kromdijk et al., 2016). However, the pleiotropic effects of this gene manipulation have yet to be examined. To …
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Structure and Function Analysis of a Plant Transcription Activator
… which confers a different expression pattern in transgenic tobacco. One such cis-element, activation sequence (as)-1 (-83 to -63) mediates preferential expression in root. The element contains a tandem repeat of the motif TGACG, that is crucial for as-1 function and binding of a tobacco nuclear …
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Role of Subcellular Differentiation in Plant Disease Resistance
… phytoalexin production and disease resistance. Transgenic tobacco, constitutively expressing the defense-inducible tomato hmgr isogene, hmg2, showed fewer and smaller lesions following tobacco mosaic virus (TMV) inoculation. There is little evidence of phytoalexins acting directly against …
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The Utilization of the Hmg2 Inducible Promoter to Genetically Engineer Parasite Resistance in Tobacco
… control of parasite-responsive promoters using tobacco (Nicotiana tabacum L. cv. Xanthi). For nematode resistance, an anti-feeding strategy was employed utilizing the tomato proteinase inhibitor I (PI-I) gene as a nematode specific toxin. Transgenic tobacco plants were generated that expressed …
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