Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"transgenic plant"”.
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Transgenic Plants, Current State, and Influence on the Environment
The techniques of plant transformation are discussed. They are bacterial-mediated, electroporation transformation, biolistic (particle bombardment), and microbeam laser. By using different strategies, foreign gene(s) from various organisms can be inserted into plant genome and expressed. …
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Design characterisation and in planta expression of a chimeric xylanase
… affecting the growth and development of the plant. Harvested biomass could then be heat-treated, activating the CAZymes and inducing hydrolysis of the biomass. Additionally, chimeric thermostable enzymes could be constructed from extremely thermophilic CAZyme domains, tailored to target …
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Reprogramming alkaloid biosynthesis in Catharanthus roseus : synthetic biology in plants
The medicinal plant Madagascar periwinkle (Catharanthus roseus) produces over 130 monoterpene indole alkaloid (MIA) natural products. Many of these compounds have pharmaceutical value, such as the anticancer agents vinblastine and vincristine. Unnatural modifications can impart novel bioactivity to …
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A putative approach for influencing stomatal density by over-expressing NADP-dependent malic enzyme in arabidopsis
<p>Stomata are leaf structures critical for plant survival because they modulate the rate of transpiration. This study will examine a possible mode for regulating stomatal density by over-expressing NADP-dependent malic enzyme (NADP-ME), which is thought to deplete the counter-anion pool in …
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Exploiting alkaloid biosynthesis in Madagascar periwinkle to obtain natural product derivatives and new biocatalysts
Plant alkaloid biosynthesis produces many natural products with medicinal value. For example, vinblastine and vincristine from Catharanthus roseus monoterpene indole alkaloid biosynthesis, and camptothecin derivatives from Ophiorrhiza pumila quinoline alkaloid biosynthesis, are anticancer agents …
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Genetic Engineering of 2,4-D Resistance in Wine Grapes
… 2,4-D easily drifts and severely affects grape plantings around areas where it is used. Since grapes have no known genetic resistance to this herbicide, breeding to incorporate resistance in commercial cultivars cannot be done. Thus genetic engineering has to be employed to produce adaptable …
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Enzyme structure, function, and evolution in flavonoid biosynthesis
Plant specialized metabolism is a key evolutionary adaptation that has enabled plants to migrate from water onto land and subsequently spread throughout terrestrial environments. Flavonoids are one particularly important class of plant specialized metabolites, playing a wide variety of roles in …
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Functional studies on the extrinsic proteins of Photosystem II
… significantly altered. Using the psbo1 mutant plant as a transgenic host, two plant lines were produced, which contained an N-terminally His6-tagged PsbO-1 protein. Photosystem II closure kinetics demonstrated that the defective double reduction of QB and the delayed exchange of QBH2 with the …
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Agrobacterium-mediated gene transfer into kiwifruit
… of Agrobacterium-mediated transformation for new plant species. A series of binary vectors have been constructed that express a chimaeric β-D-glucuronidase (GUS) gene in plants cells but not in bacterial cells. This feature allows GUS activity from transformed plant cells to be assayed in the …
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Studies on improvement of Chinese wheat cultivars for resistance against an endemic virus using transgenesis and virus-wheat interactions at the molecular and ultrastructural levels
… of a chimeric pglucuronidase (<i>uid</i>A) gene. Transgenic wheat for the model variety Bob White and three Chinese semi-winter wheat cultivars were obtained by microprojectile bombardment of pre-cultured immature embryos using achimeric bar gene as the selectable marker. Plants were regenerated …
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Studies of Resistance to Aphids and Viruses in Arabidopsis thaliana and Capsicum annuum
… production. In this study, methods to enhance plant resistance to CMV infection and infestation by Myzus persicae, a vector that transmits CMV, were assessed. There were two parts to the project. In the first, the feasibility of using exogenous salicylic acid (SA) application to induce …
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Plant-derived Murine IL-12 and Ricin B-Murine IL-12 Fusions
… goals of this research were to determine whether plants can serve as an effective production system for bioactive IL-12, a complex 70kDa glycoprotein cytokine, and whether the plant lectin RTB can facilitate mucosal delivery of IL-12 to immune responsive sites. Transgenic tobacco plants expressing …
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Tamarillo mosaic potyvirus: characterization and resistance
… regions. Viruses were detected by indicator plant symptomology, and the incidence confirmed by dot blot analysis or ELISA. TaMV was present in 100% of tamarillo trees analyzed in ten of the twelve orchards surveyed. Incidence of potato aucuba mosaic potexvirus, cucumber mosaic cucumovirus, …
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Expression of Human Protein C in Transgenic Tobacco
… g-carboxylation, and N-linked glycosylation. Plants have the eukaryotic protein modifying mechanisms required for many human proteins and may provide a safe, cost-effective system for producing hPC on a large-scale basis. Tobacco (Nicotiana tabacum L.) is particularly well suited for use as a …
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Identification of a gene that makes-plants-gigantic-1: characterization of mpg1, a novel mutant of rice
… methods to generate additional fuel and food. Plants play a unique role in this challenge, as they offer a means to create sustainable sources of energy as well as provide a source of food. It is important to investigate ways to increase plant productivity so that society can dually and …
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Expression of the Human Milk Protein, Beta-Casein : in Transgenic Potato Plants
… p-casein gene and its transcript in regenerated transgenic plants were confirmed by PCR and RT-PCR analysis respectively. Human p-casein protein was detected in leaves of transgenic plants by immunoblot analysis. The p-casein protein produced by the plants migrated as a single band with a …
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GENETIC MANIPULATION OF CYTOKININ LEVELS IN NEW PLANT TYPE RICE
… in the top three leaves of field-grown New Plant Type (NPT) rice were determined during the grain filling period. The chlorophyll content in the leaves decreased from flowering to maturity. Fast-senescing and slow-senescing NPT rice lines were identified. The presence of 22 different CKs in …