Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"transgenic cells"”.
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Transgenic manipulation of polyamine metabolism in poplar
… and related pathways in transformed poplar cells overexpressing a mouse odc cDNA under the control of 2X 35S CaMV promoter. The transgenic cells (line 2E) showed elevated levels of mouse ornithine decarboxylase enzyme activity, several fold higher amounts of putrescine, a small increase in …
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Understanding polyamine metabolism through transgenic manipulation in poplar suspension cultures
… on several aspects of cellular metabolism. The transgenic cells were compared with a control cell line that was transformed with the beta-glucuronidase (GUS) gene.</p><p>It was observed that enhanced putrescine metabolism resulted in: (i) increased expression of arginine decarboxylase gene, …
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Optimization of an Immune Assay to Detect Binding of NOD2 to Staphylococcus Aureus
… on the surface of and inside innate immune cells. This study aimed to design and optimize an assay that can be utilized to investigate <em>S. aureus</em> components that contribute to the activation of NOD2 receptors. The assay was designed using one wild type and two bacterial strains from …
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A microfluidic culture for two populations of dorsal root ganglia for differential staining
… for applications ranging from patterning of cells and biomolecules to control of local environment factors such as temperature, which requires external pumping or other applied forces. We demonstrated a pump-free device that exploits the surface energy stored in a liquid droplet to pump …
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A T cell receptor transgenic approach to the study of thymocyte development and autoimmune disease
… and negative selection of T cell receptor-transgenic thymocytes. It was found that peptides with limited amino-acid sequence homology and vastly different affinity values for the T cell receptor could promote positive and negative selection of T cells in a dose dependent manner. It was …
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Modelling neurodegenerative diseases in human iPSC-derived neurons
… in this work highlighted that purification of transgenic cells is a key bottleneck to gene editing. I therefore adapted a synthetic marker gene that presents a streptavidin binding peptide (SBP) tag on the extracellular membrane, detailed in Chapter 3. Expression of this tag in iPSCs enabled …
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Membrane Domain of Plant 3-Hydroxy-3-Methylglutaryl Coenzyme A Reductase: Targeting, Topology, and Function
… in defense responses to elicitor treatment. BY-2 cells showed minimal defense capabilities, whereas KY-14 cells were rapidly induced as indicated by increased HMGR enzyme activity and browning of the cells. HMGR enzyme activity was decreased in both KY-14 and BY-2 cells following sterol treatment, …