Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 129 for “"transgenes"”.
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Konditionale Inaktivierung von Peroxisomen im Nervensystem der Maus : ein transgenes Modell einer humanen Leukodystrophie
Die juvenile Form der X-chromosomal vererbten Adrenoleukodystrophie ist durch eine progressive, entzündliche Demyelinisierung der cerebralen weißen Substanz bei gleichzeitiger Nebenniereninsuffizienz gekennzeichnet. Der zugrunde liegende genetische Defekt ist die Mutation eines peroxisomalen …
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Changes in large-scale chromatin structure and dynamics of BAC transgenes during transcriptional activation
Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by William Ingram (wingram2@illinois.edu) on 2010-05-14T20:52:51Z Item is restricted until 2012-05-14T20:52:43Z
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Inheritance and expression of Cry3Aa and PVY-O coat protein transgenes in diploid and tetraploid potato
The potential benefits of plant genetic engineering for disease and pest resistance have been widely acknowledged in many studies, and although genetically modified crops are still encountering public wariness, these benefits warrant continued exploration. Because of its intrinsic economic …
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Improvement of expression of recombinant human protein C in the milk of transgenic animals using a novel transgene construct
… studies of mammary tissue specific expression of transgenes using the murine whey acidic protein (WAP) promoter have shown widely variable, position-dependent and copy number-dependent expression. This study evaluates a series of three WAP transgenes containing the cDNA of human protein C (hPC) …
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Effect of Ploidy Elevation, Copy Number and Parent-Of-Origin on Transgene Expression in Potato
… Little is known regarding the stability of transgenes through sexual polyploidization in potato, although studies have associated ploidy elevation with transgene silencing in plants such as Arabidopsis thaliana. In the present study, potato was transformed with two different transgenes, …
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Adeno-associated virus rep78 expression in Arabidopsis thaliana
… event for site-specific integration of transgenes in plants. This strategy would alleviate problems associated with current plant transformation methods that integrate transgenes randomly. The AAV-2 gene encoding the enzyme that catalyzes the insertion (rep78) was introduced into …
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Bruno regulates mRNA translation by binding to multiple sequence motifs
… known to regulate during oogenesis. GFP reporter transgenes under the control of the UAS-Gal4 expression system were constructed with each class of the binding sites within the reporter transgenes’ 3’UTRs to test the motifs’ ability to repress the reporters in vivo. In a wildtype background, the …
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Transgene-free strategies for wireless control of animal physiology using magnetite nanoparticles
… stimulation has often relied on transgenes, but by choosing tissues that endogenously express the proteins required to detect the physical stimuli (like heat or force) produced by the nanoparticles, it is possible to avoid the need for transgenes. Not relying on transgenes …
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Rox1 function in dosage compensation: structural / functional analysis of a non-coding rna
… them. Previous studies of roX1 mutants and transgenes have identified a large region at the 5' end of roX1 that is necessary for X-localization of the MSL complex. To dissect the function of this region, roX1 transgenes containing portions of the 5' end were generated and analyzed. Multiple …
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Agrobacterium-Mediated Transformation of Soybean: Test of Selectable Marker Genes
… analyses confirmed the integration of the transgenes into the soybean genome. Progeny analyses of these transgenic lines demonstrated that the transgenes were inherited in a Mendelian fashion and the transgenic plants showed resistance to the corresponding selection agents, glufosinate and …
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Analyzing the Importance of Phosphorylation and Ubiquitination of Amino Acids in the Enhancer of Rudimentary Protein, ERH
… has been analyzed through the creation of transgenes with codon changes at the identified sites intended to either prevent or mimic phosphorylation or ubiquitination. Upon insertion of the e(r) transgenes into the Drosophila melanogaster genome, they have been crossed with two lethal and …
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Mechanisms and functional significance of nuclear compartmentalization
… I showed an actin dependent association of HSP70 transgenes with nuclear speckles after heat shock. I visualized HSP70 transgenes moving curvilinearly towards nuclear speckles over ~0.5-6 μm distances at velocities of 1-2 μm min-1. Observation of chromatin stretching in the direction of movement …
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Feedback Regulation of Wnt Signaling by Naked Cuticle (Nkd) During Drosophila Embryogenesis
… to wild type embryos, and overexpression of Nkd transgenes in nkd mutants did not alter Dsh distribution or abundance by confocal microscopy. Nkd transgenes lacking Dsh-binding regions were mostly able to rescue nkd mutants, suggesting that the Dsh-binding regions of Nkd contribute little to Nkd …
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BAC transgene arrays as a model system for studying large-scale chromatin structure
… regions consisting of tandem repeats of BAC transgenes, which contain 150-200 kb of defined genomic regions, and used them as a model system to study the mechanisms and functional significance of large-scale chromatin organization. The BAC transgene arrays recapitulated several important …
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Analysis of the Expression Profiles of Two Isoforms of the Antifungal Protein Osmotin from Gossypium hirsutum
… analyses indicated the absence of the osmotin transgenes, but the presence of GUS genes from the vector cassette. Alkaline blot analyses of the vector DNAs from transformed Agrobacterium cells confirmed that an anomalous DNA structural rearrangement or aberrant recombination event probably …
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Epigenetic repression of intronless mobile elements by the HUSH complex
… is unknown. By monitoring transcription from L1 transgenes, I found that incoming L1s are recognized by HUSH independently of their integration sites, a critical difference with previously studied viral silencing. By studying sequence determinants of L1 repression by HUSH, I discovered that HUSH …
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TAXI : a new vehicle for the transfer of genes into monocotyledonous plants
… successful transformation and integration of the transgenes remained doubtful, because the frequency of kanamycin resistance in the progenies ( Rl , R2 and R3 ) did not increase. To achieve a real transformation and heritable transgenic rye, a new vehicle for gene transfer to plants was developed. …
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Effects of Changes of Redox Properties of the Chloroplast ATP Synthase in Arabidopsis
… mutated gene constructs of gamma-subunit. The transgenes were overexpressed behind a constitutive 35S promoter compared with the endogenous gamma-subunit gene. In the isolated mutant chloroplasts, the high ATPase activities did not require DTT treatment while in the wild-type plant, ATPase …
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The effect of potential anticancer gene products on breast cancer cell lines
… as well as engineering and expression of transgenes in a cellular production system. Therefore, my thesis project required the isolation of anti-cancer genes from either human genomic or cDNA libraries, verification of sequence fidelity, construction of transgene expression cassettes, and …
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A recombineering pipeline for functional genomics applied to Caenorhabditis elegans
… engineering of genomic BAC clones into tagged transgenes for integrative transformation. C.elegans offers unique advantages for function discovery through protein tagging: compact and a well annotated genome, combined with a simple and well-understood anatomy and pattern of development. …
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