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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.

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Showing 1 to 20 of 78 for “"transgene expression"”.

  1. HIV-derived lentiviral vectors engineered for regulated transgene expression

    Guarino, Barbara MariaETH2005

    ethz Repository record

  2. Effect of Ploidy Elevation, Copy Number and Parent-Of-Origin on Transgene Expression in Potato

    Johnson, Alexander Arthur TheodoreVirginia Tech2001

    … considerably decrease production costs through transgene-mediated resistance to insect, viral, fungal and bacterial pathogens. Potato can be produced from true potato seed (TPS) through a sexual polyploidization step, known as 4x-2x hybridization. Little is known regarding the stability of …

    vt Repository record

  3. Creating a versatile toolkit for transgene expression using BACS and for dissecting large-scale chromatin organization

    Zhao, BinhuiUniversity of Illinois at Urbana-Champaign2019

    … and position effect variegations observed in transgene expressions, which, as well as other epigenetic silencing mechanisms, have been obstacles to achieving predictable and stable transgene expression. Molecular dissections of the determinants regulating large-scale chromatin organization …

    uiuc Repository record

  4. Lactogenesis Induction in Transgenic Virgin Pigs as a Model for Identifying Transgene Expression and Recombinant Protein Production

    McCourt, Shannon M.Virginia Tech1998

    … of recombinant proteins by directing a transgene to the mammary gland with a milk protein gene promoter. In order to determine whether or not the protein will be expressed, the animals must be maintained at least through their first lactation. An experiment was performed to determine if …

    vt Repository record

  5. Optimizing nuclear transgene expression and genome editing for high-value compound production in the green microalga Chlamydomonas reinhardtii

    Jacobebbinghaus, NickUniversität Bielefeld

    … engineering of this host. However, nuclear transgene expression rates remain rather low compared to heterotrophic cultivated bacteria or yeast, displaying a major limitation for the use of the alga in biotechnology. Especially, the complex eukaryotic genome structure and transgene silencing …

    bielefeld Repository record

  6. Creation of Versatile Cloning Platforms for Transgene Expression and Epigenome Editing and Their Application to Pancreatic Islet Biology

    Haldeman, Jonathan Mark2018

    … previously reported that adenovirus-mediated overexpression of the transcription factor PDX1 is sufficient to induce β-cell replication, but underlying mechanisms remain to be resolved. Using statistical modeling, we identified the miR-17 family, a member of the miR17~92 miRNA cluster, as a …

    duke Repository record

  7. The In Vitro Transgene Expression and In Vivo Transgene Integration of Condensed DNA Injected into the Cytoplasm of Murine Zygotes

    Dunlap-Brown, MaryaVirginia Tech2010

    … five analyses. Southern Analysis detected 2.8% transgenesis. Cytoplasmic injection of linear CMV-EGFP (625 µg/ml in water) was 3.7% transgenic. Pronuclear injections produced 7.9% transgenesis. This research identified a range of reversible electroporation that could easily be verified <i>in …

    vt Repository record

  8. OPTIMIZING GENE THERAPY: THE IMPACT OF SMALL MOLECULE MODULATORS ON ADENO-ASSOCIATED VECTOR TRANSDUCTION AND INTEGRATION

    Kasimsetty, Aradhana2025

    … to AAV gene therapy can be inefficient transgene expression from AAV vectors. One approach to improve AAV transduction has been to combine the vector with small molecule modulators that disrupt cellular pathways, which can also impact AAV integration. Recombinant AAV genomes primarily …

    penn Repository record

  9. Developing an astrocyte-selective AAV-ADAMTS4 gene therapy to promote repair after spinal cord injury

    Griffin, JarredResearchSpace@Auckland2018

    … therapy is a method that allows for long-term expression of therapeutic molecules. Adeno-associated viral (AAV) vector gene therapy, in particular, has emerged as the vector of choice for safe, robust and long-term transgene expression in the central nervous system (CNS). Since astrocytes play …

    auckland-ms Repository record

  10. Maize Transformation for Resistance to Aspergillus Flavus

    Mei, ChuanshengUniversity of Illinois at Urbana-Champaign2015

    … and then self-pollinated up to six times. The transgenes have been confirmed to be integrated into the genome by PCR and Southern blots and the transgene expression detected by RT-PCR, Northern and Western blots, and enzyme activity assays. The ch18 transgene was expressed in embryos as …

    uiuc Repository record

  11. One- and Two -Photon States for Quantum Information

    Peters, Nicholas A.University of Illinois at Urbana-Champaign2015

    The expression of the transgene gus was studied in all 40 callus lines, 12 regenerated T0 plants and the T1 and T2 progenies of 5 lines. The isogenic SC lines had an average expression level based on the activity of beta-glucuronidase of 158 +/- 9 units/mg protein (mean +/- SEM; n=3; variance …

    uiuc Repository record

  12. Global Expression Profiles and Targeted Pathway Modulation During Soybean Embryo Development

    Thibaud-Nissen, FrancoiseUniversity of Illinois at Urbana-Champaign2015

    … by real-time PCR. Three lines showed low transgene expression but increased transcript abundance for two endogenous CHS genes One line showed a 10-fold increase in CHS expression over the control seeds, but no change in isoflavone content, and is a candidate for more extensive profiling of …

    uiuc Repository record

  13. Novel Approaches to Transgenesis in the Teleost Medaka (Oryzias latipes)

    Grabher, ClemensUniversität Heidelberg

    … generation of transgenic medaka fish. General transgenesis including transient expression of reporter genes and germ line integration of reporter genes was improved by application of two novel techniques. In addition, one of these methods allows for the first time efficient enhancer trapping in …

    heid-diss Repository record

  14. Ex vivo culture of patient tissue and examination of gene delivery

    Rajendran, SimonUniversity College Cork2014

    … factors and tissue specific effects on vector expression. Adenoviral vectors under the control of the human CXCR4 promoter demonstrated a 'tumour on' and 'normal off' expression profile when compared with the ubiquitously active CMV promoter when tested in patient tumour tissue. In addition, we …

    cork Repository record

  15. Poly(glycoamidoamine)s: Understanding their Structure and Structure-Bioactivity Relationships

    Taori, Vijay P.Virginia Tech2010

    … integrity of the sustained released pDNA for the transgene expression was characterized with an assay to see the expression of the green fluorescent protein (GFP) from the <strong>T4</strong>/GFP-pDNA layer-by-layer constructs. PGAAs show a very efficient delivery of the pDNA in a non-toxic …

    vt Repository record

  16. Regulation of Memory by the Actin-Binding Protein Cofilin

    Merovitch, Neil H2023

    … three objectives to determine how cofilin overexpression in vivo affects the actin cytoskeleton and memory. I first characterized two novel transgenic mouse models that allow for either overexpression of wild-type or constitutively inactive forms of cofilin with spatial and temporal regulation. …

    toronto-retro Repository record

  17. Interleukin-1 receptor antagonist delivery through adenoviral mediated gene transfer as a treatment for equine joint disease

    Frisbie, David D., author, McIlwraith, C. W., advisor, Chapman, Phillip L., committee member, Evans, C. H., committee member, Oxford, J. R., committee member, Trotter, G. W., committee memberColorado State University. Libraries1999

    … conducted to assess both ability to deliver a transgene to the joints of horses, and the specific effects overproduction of IL-1Ra using gene transfer would have on an experimental model of joint disease in the exercising horse. Using the published gene sequence for equine IL-1Ra, an adenoviral …

    colostate Repository record

  18. GENETIC MANIPULATION OF STORAGE ROOT DEVELOPMENT IN HORTICULTURAL CROPS

    RAHMAN MILAN, A. B. D.De Montfort University2002

    … the Norman Borlaug Institute laboratories. The expression of this gene is associated with dividing tissue, particularly the secondary vascular cambial rings of the sugar beet storage root. This gene is of interest for two reasons. As a plant-specific regulatory protein, it could play an …

    de-montfort Repository record

  19. Receptor Mediated Oral Delivery Of Bioencapsulated Green Fluorescent Protein Expressed In Transgenic Chloroplasts

    Limaye, Arati

    … soluble chloroplast stromal compartment. Protein expression through chloroplast transformation system offers a number of advantages over nuclear transformation such as a high level of transgene expression (up to 47% of the total soluble protein), due to the presence of 10,000 copies of the …

    ucf

  20. Transcriptional targeting of lentiviral vectors to the erythroblastic progeny of hematopoietic stem cells

    Lotti, FrancescoThe Open University2003

    … stem cells (HSC), and regulation of transgene expression in specific cell lineages. HIV-derived lentiviral vectors are very effective in transducing rare, non-dividing stem cell populations without altering their long-term repopulation and differentiation capacity. We developed a …

    the-open-u Repository record

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