Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 395 for “"transgene"”.
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Construction of a COL11A1 Transgene Vector
… cDNA in a cartilage-specific manner. This transgene will be used in future studies to correct the type XI collagen defect in homozygous cho mice. 2) To place the cDNA in an in vitro expression vector to be used for in vitro transcription/translation assays. Methods and Results: Through the …
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The In Vitro Transgene Expression and In Vivo Transgene Integration of Condensed DNA Injected into the Cytoplasm of Murine Zygotes
… five analyses. Southern Analysis detected 2.8% transgenesis. Cytoplasmic injection of linear CMV-EGFP (625 µg/ml in water) was 3.7% transgenic. Pronuclear injections produced 7.9% transgenesis. This research identified a range of reversible electroporation that could easily be verified <i>in …
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Transgene Mausmodelle zur Charakterisierung der Funktion kardialer beta-adrenerger Rezeptoren
… Rezeptoren mit Hilfe einer Kombination aus transgenen Mausmodellen und physiologischen und molekularbiologischen Methoden untersucht. Durch gezielte Überexpression des humanen beta1-adrenergen Rezeptors im Herzen transgener Mäuse konnte gezeigt werden, daß die chronische Aktivierung dieses …
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PERPETUATION OF NON-GENETIC CHANGES AT A TRANSGENE LOCUS IN C. ELEGANS
… the nematode worm C. elegans that shows robust transgenerational expression like many loci in the genome but, unlike other tested loci, can be uniquely susceptible to transgenerational silencing by one of two distinct processes. This locus could be silenced by double-stranded RNA (dsRNA) …
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Transgene-free strategies for wireless control of animal physiology using magnetite nanoparticles
… stimulation has often relied on transgenes, but by choosing tissues that endogenously express the proteins required to detect the physical stimuli (like heat or force) produced by the nanoparticles, it is possible to avoid the need for transgenes. Not relying on transgenes …
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Using a Mammalian Virus to Create Plants for Site-Specific Transgene Insertion
… a specific integration site for the insertion of transgenes using features of the adeno-associated virus (AAV) life cycle. In the absence of helper virus, AAV integrates into a specific location on human chromosome 19, the AAVS1 locus. The sequence for AAV integration was introduced into the model …
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Silencing Seed Dormancy Genes to Mitigate Risk of Transgene Flow to Weedy Rice
<p>The flow of fitness-enhancing transgenes from genetically modified crops into wild/weed relatives may cause serious ecological and economic consequences. Seed dormancy (SD) is a key adaptive trait that distributes germination over time, resulting in weed persistence in agroecosystems. Thus, …
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BAC transgene arrays as a model system for studying large-scale chromatin structure
… regions consisting of tandem repeats of BAC transgenes, which contain 150-200 kb of defined genomic regions, and used them as a model system to study the mechanisms and functional significance of large-scale chromatin organization. The BAC transgene arrays recapitulated several important …
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Ligand-Directed Aavp to Deliver A Therapeutic Transgene to Neuroendocrine Tumors of The Pancreas
<p>Human pancreatic neuroendocrine tumors are rare tumors that form in the endocrine pancreas. Patients with these tumors have limited therapeutic options, and curative intervention is limited to surgical resection. These tumors do, however, consistently express somatostatin receptor type 2 (SSTR2) …
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Effect of Ploidy Elevation, Copy Number and Parent-Of-Origin on Transgene Expression in Potato
… considerably decrease production costs through transgene-mediated resistance to insect, viral, fungal and bacterial pathogens. Potato can be produced from true potato seed (TPS) through a sexual polyploidization step, known as 4x-2x hybridization. Little is known regarding the stability of …
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Creating a versatile toolkit for transgene expression using BACS and for dissecting large-scale chromatin organization
… and position effect variegations observed in transgene expressions, which, as well as other epigenetic silencing mechanisms, have been obstacles to achieving predictable and stable transgene expression. Molecular dissections of the determinants regulating large-scale chromatin organization …
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Chromosomal localization of a proinsulin transgene inserted with a transposon-based vector into Japanese quail, Coturnix coturnix
… and chromosomal identification of proinsulin transgene insertions by laser microdissection and nucleotide sequence from G2 Japanese quail. The 28S rDNA was found on a macrochromosome and a microchromosome pair by primed in situ hybridization, fluorescent in situ hybridization, and silver …
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Genetic Transformation of Switchgrass (Panicum Virgatum L.) with Endoglucanase Gene and Characterization of Plants with Endoglucanase Transgene
As a warm season grass native to the North American continent, switchgrass is considered as one of the most promising biofuel crops in the USA. It is a C4 plant that makes it energy efficient. Switchgrass has a deep root system that allows it to grow on marginal land with low water and nutrient …
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Lactogenesis Induction in Transgenic Virgin Pigs as a Model for Identifying Transgene Expression and Recombinant Protein Production
… of recombinant proteins by directing a transgene to the mammary gland with a milk protein gene promoter. In order to determine whether or not the protein will be expressed, the animals must be maintained at least through their first lactation. An experiment was performed to determine if …
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Optimizing nuclear transgene expression and genome editing for high-value compound production in the green microalga Chlamydomonas reinhardtii
… engineering of this host. However, nuclear transgene expression rates remain rather low compared to heterotrophic cultivated bacteria or yeast, displaying a major limitation for the use of the alga in biotechnology. Especially, the complex eukaryotic genome structure and transgene silencing …
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Creation of Versatile Cloning Platforms for Transgene Expression and Epigenome Editing and Their Application to Pancreatic Islet Biology
<p>Insulin secreting β-cells within the pancreatic islets of Langerhans are vital to maintaining glycemic control. β-cell functional mass is lost during the progression to both Type 1 and Type 2 diabetes mellitus, resulting in hyperglycemia. Therefore, a major goal of diabetes research is to …
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Improvement of expression of recombinant human protein C in the milk of transgenic animals using a novel transgene construct
… studies of mammary tissue specific expression of transgenes using the murine whey acidic protein (WAP) promoter have shown widely variable, position-dependent and copy number-dependent expression. This study evaluates a series of three WAP transgenes containing the cDNA of human protein C (hPC) …
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Soybean Mosaic Virus Hc-Pro Enhances Viral Symptoms, Bean Pod Mottle Virus and Transgene RNA Stability, siRNA Accumulation, and Reduces Soybean Seed Production
… the expression of a beta-glucuronidase (GUS) transgene that was susceptible to PTGS. SMV infection stabilized GUS gene expression, enzyme activity, and enhanced GUS siRNA accumulation.
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Inheritance and expression of Cry3Aa and PVY-O coat protein transgenes in diploid and tetraploid potato
… a transgenic crop would affect the behavior of a transgene in the resultant progeny. In the initial part of this study, transgenic lines were developed with a Cry3Aa transgene. These plants were then used in 4x-4x reciprocal crosses and 4x-2x hybridization schemes to determine the stability of the …
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