Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 64 for “"transfection efficiency"”.
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Understanding Non-viral Nucleic Acid Delivery Vehicles with Different Charge Centers and Degradation Profiles
… affect toxicity, release of nucleic acid and transfection efficiency. Poly(glycoamidoamine)s (PGAAs) contained different carbohydrate and secondary amine moieties and showed high transfection efficiency to different cell lines in a nontoxic manner. The "proton sponge hypothesis" has attempted …
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Mechanisms of Cytotoxicity and Intracellular Trafficking for Gene Delivery Polymers
… end groups of trehalose-containing polymers on transfection efficiency, and the effect of different linker lengths between galactose and a hepatocyte-targeted polymer on transfection efficiency were studied. Furthermore, it was demonstrated that polymers with terbium chelated in their repeat …
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Regulated release of a novel non-viral gene delivery vector from electrospun coaxial fiber mesh scaffolds
… had significantly reduced toxicity and improved transfection efficiency with multipotent human mesenchymal stem cells. This transfection efficiency could further be modulated by changing the concentration of sodium chloride and temperature used to assemble PEI-HA/DNA complexes. To facilitate the …
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The effects of PVP(FE(III)) catalyst and polymer molecular weight on gene delivery via biodegradable crosslinked polyethylenimine
… polyethylenimines (PEI) that show high transfection efficiency and low toxicity by cross-linking 800 Da PEI with diacrylate cross-linkers using Michael addition. However, the synthesis was difficult to control, inconsistent, and resulted in polymers with a narrow range of molecular …
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Design of hybrid retroviral/synthetic gene delivery vectors
… difficult to modify without significant loss in efficiency. Non-viral vectors are typically non-pathogenic and non-immunogenic, yet lack the efficiency necessary for gene therapy and are also typically toxic at clinically useful concentrations. It is clear that the current implementation of gene …
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Design of Lipid and Polymeric Carriers for Nucleic Acid Delivery
… <p>Cationic lipids are most commonly used transfection reagents in delivery of nucleic acids to target cells <em>in vitro</em>. In Chapter 3, we synthesized a series of pyridinium lipids which contain a heterocyclic ring and a nitrogen atom. The structure–activity relationship (SAR) and …
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Cationic liposome-mediated transfection of mammary epithelial cells with AAV-based plasmid DNA
… delivery of AAV-based plasmid DNA to enhance transfection efficiency, and the molecular characteristics of AAV-based plasmid DNA as a vector for transfection and integration of DNA into the genome. Chemical-mediated procedures were used to deliver plasmid DNA to the mouse mammary epithelial …
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Flow-Through Electroporation in Asymmetric Curving Microfluidic Channels
… We show that the electric parameters affect the transfection efficiency and cell viability. Higher electric field intensity results in higher transfection efficiency. This is also true in the cases with longer electroporation duration time. In our experimental work, we executed flow-through …
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Engineering of Nonviral Vectors for Gene Therapy
… was able demonstrate a 2--3 fold enhancement in transfection efficiency in insulin receptor-rich cell lines.
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Characterization of Bioreducible Poly(B-amino Ester) Nanoparticles for siRNA Delivery
… siRNA release system to increase safety and transfection efficacy. We hypothesized that the addition of disulfides to the main backbone of PBAE NPs would reduce cytotoxicity and increase siRNA transfection efficacy. Glutathione, a reducing agent present in the cytosol, can reduce a disulfide …
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Materials and biological approach to gene delivery in human embryonic stem cells
… develop new methods and protocols to enhance the efficiency of gene delivery to be useful. For the materials aspect, diblock copolymers consisting of poly(ethylene glycol)-block-poly(γ-4-(((2-(piperidin-1-yl)ethyl)amino)methyl)benzyl-L-glutamate) (PEG-b-PVBLG-8) were synthesized and evaluated for …
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ENGINEERING LIPID AND HYBRID NANOVECTORS FOR TARGETED GENE DELIVERY TO MACROPHAGE SUBPOPULATIONS
… modulate cellular uptake and/or improve the transfection efficiency of the resulting nanovectors (Chapter 1). The experimental part of this thesis is organized presenting first the technological data of the prepared lipopolyplexes (Chapter 2), PLA-PEG/lipid nanoparticles (Chapter 3) and LNP …
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Success and limitations of gene therapy with growth factors to enhance cutaneous wound healing
… problem, several alternative means of improving transfection efficiency were assessed. The use of viral vectors was excluded because of their potential toxicity. Non-viral approaches were concentrated upon. Canonic lipofection and polyfection, effective in vitrv, were found to be of no use in …
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Method Optimization and Formulation of Chitosan Nanoparticle for PRPF31 Gene Delivery
… the loading capacity, encapsulation efficiency, protective effect on the pDNA, in vitro transfection efficiency and cytotoxicity were evaluated. The particle size, PDI, and zeta potential of chitosan nanoparticles were 182.28 ± 6.23 nm, 0.281 ± 0.002, and +22.33 ± 8.05 mV, …
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Synthesis, characterization, and evaluation of photo-active amphiphiles for gene delivery applications
… the obstacles in the cellular uptake and transfection pathways which impede nonviral gene delivery, novel lipids, polymers, and dendrimers are being engineered. Cationic lipid vectors have been widely characterized as gene delivery tools as they electrostatically interact with the anionic …
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Syntheses, structures and properties of novel poly(amino ester)s
… demonstrated to show low cytotoxicity and good transfection efficiency for DNA delivery comparable to polyethylenimine (PEI). Furthermore, some hyperbranched poly(amino ester)s can emit blue fluorescence. These poly(amino ester)s should be applicable for safe and efficient gene and drug delivery …
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Reconfiguring the side-chain functionality of cationic helical polypeptides toward maximized gene delivery capabilities
… mechanism, and thus possessed notably higher transfection efficiencies. Elongating the hydrophobic side chain also potentiated the membrane activities of the polypeptides, while at the meantime caused higher cytotoxicities. Upon an optimal balance between membrane activity and cytotoxicity, …
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Development of Aerosolizable mRNA Incorporated Chitosan Nanoparticles for Efficient Lung Delivery
… achieve localized therapeutic effects with high transfection efficiency. The research developed an in-house, cost-effective mRNA extraction method followed by its quantification and even purification if required. The mRNA-incorporated CS-TPP nanoparticles were produced through the Ionotropic …
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Materials and biological approach to gene delivery in human embryonic stem cells
… develop new methods and protocols to enhance the efficiency of gene delivery. For the materials aspect, diblock copolymers consisting of poly(ethylene glycol)-block-poly(γ-4-(((2-(piperidin-1-yl)ethyl)amino)methyl)benzyl-L-glutamate) (PEG-b-PVBLG-8) were synthesized and evaluated for their ability …
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Magnetic targeted drug delivery system in gene therapy
… studies turn to the nonvirus vectors, which have transfection efficiency and low cytotoxicity. PLLA(poly(L lactic acid)) can be biodegradable and has high biocompatibility. PLLA is already used in functional biomedical materials, including functional gene vectors. This thesis covers the synthesis …
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