Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 484 for “"transfection"”.
-
Transfection and Transformation With Bacteriophage-T4 Dna
Made available in DSpace on 2014-12-11T18:16:50Z (GMT). No. of bitstreams: 1 7212082.pdf: 2102650 bytes, checksum: 1c6e5387798bc6a2aa7e570cca6bac07 (MD5) Previous issue date: 1971
-
Microfluidic systems for cell transfection using sonoporation and electroporation
… research presents a microfluidic system for cell transfection using sonoporation and electroporation. This research has studied, designed, developed and tested the sonoporation system and electroporation system with certain biological cells. Ultrasonic standing waves, previously used for …
-
Structure-Property-Transfection Relationships in Polycation-mediated Non-viral DNA Delivery
… understand the underlying structure-transfection relationships involved in polycation-mediated gene delivery. Efforts focused on the influence of molecular weight, macromolecular topology, carbohydrate modifications, and charge density on the overall transfection activity in vitro. …
-
Understanding the biological barriers to Jurkat Transfection using non-viral peptides
… are hard to transfect, the key barriers to the transfection of primary T-cells should be explored for further rational designs of transfection agents. The low level of heparin sulfate proteoglycans on the cell surface of Jurkat and primary T-cells weakens the cell entry of cationic …
-
Extended transient transfection by repeated delivery of in vitro-transcribed RNA
… the rate at which cells recover from ivT-RNA transfection by combined knockdown of innate-immune-related genes was developed. Using this technique a high, approximately steady-state level of protein expression was transduced in MRC-5 human fetal-lung fibroblasts by repeated transfection with …
-
A Microfluidic Device for Transfection of Mammalian Cells Using Adjustable Shear Stress
… damage. The advantage of this physical method of transfection is the possibility of incorporating large molecules that cannot be inserted with more traditional chemical transfection methods, while avoiding the large fall in viability seen with other physical methods such as electroporation. Unlike …
-
Cationic liposome-mediated transfection of mammary epithelial cells with AAV-based plasmid DNA
… delivery of AAV-based plasmid DNA to enhance transfection efficiency, and the molecular characteristics of AAV-based plasmid DNA as a vector for transfection and integration of DNA into the genome. Chemical-mediated procedures were used to deliver plasmid DNA to the mouse mammary epithelial …
-
Modified alginates as a matrix for gene transfection in a HeLa cell model
… greatest limiting factors in gene therapy and transfection methodology. A number of synthetic vectors such as cationic polymers and liposomes have been developed for introducing 'therapeutic' genetic material into the cell. These approaches have the distinct advantage over viral vectors in …
-
Synthesis and biological studies of a fullerene-Taxol conjugate and fullerene-based transfection vectors
… to the design and synthesis of C60-based transfection vectors in order to establish a structure-activity relationship (SAR) profile for this class of compounds. Several positively-charged derivatives of C60 were synthesized via Bingel and Prato chemistry and tested for transfection …
-
Understanding Non-viral Nucleic Acid Delivery Vehicles with Different Charge Centers and Degradation Profiles
… affect toxicity, release of nucleic acid and transfection efficiency. Poly(glycoamidoamine)s (PGAAs) contained different carbohydrate and secondary amine moieties and showed high transfection efficiency to different cell lines in a nontoxic manner. The "proton sponge hypothesis" has attempted …
-
Multifunctional bioreducible nanoparticles for gene therapy
… of the delivery vector, leading to increased transfection efficiency and decreased cytotoxicity. </p> <p>In this dissertation, we first evaluated the effect of enhanced reductive disassembly on transfection activity of plasmid DNA and antisense oligonucleotide (AON) polyplexes using on a …
-
Regulated release of a novel non-viral gene delivery vector from electrospun coaxial fiber mesh scaffolds
… had significantly reduced toxicity and improved transfection efficiency with multipotent human mesenchymal stem cells. This transfection efficiency could further be modulated by changing the concentration of sodium chloride and temperature used to assemble PEI-HA/DNA complexes. To facilitate the …
-
Construction of inducible expression systems for controlled transfer of Agrobacterium tumefaciens T-DNA in plant transient expression systems /
… using Agrobacterium-mediated transient transfection of plants. However, transfection methods suitable for open field applications are still desirable as a cheaper alternative. Biosafety concerns related to the use of recombinant agrobacteria in an industrial transfection process include …
-
Systems Engineering for Viral Vector Manufacturing
… of meeting current and future demands. Transient transfection via triple transfection of plasmids is the most common manufacturing platform for the clinical stage due to its flexibility in changing the plasmids and transgene. Triple transfection has low yields and low fraction of full capsids in …
-
IL -10 gene therapy for the treatment of pulmonary inflammation
… sulfate for their ability to promote gene transfection in macrophages. We have developed an efficient transfection protocol which is at least 20--25 fold superior to frequently used protocols for macrophage transfection. However, gene transfection in macrophages is still low. Since …
-
Mechanisms of Cytotoxicity and Intracellular Trafficking for Gene Delivery Polymers
… end groups of trehalose-containing polymers on transfection efficiency, and the effect of different linker lengths between galactose and a hepatocyte-targeted polymer on transfection efficiency were studied. Furthermore, it was demonstrated that polymers with terbium chelated in their repeat …
-
Gene-supplemented collagen scaffolds for non-viral gene delivery for brain tissue engineering
… long-term release and overexpression of GDNF via transfection of cells seeded into the scaffold or endogenous cells. The first part of the thesis aimed to investigate the in vitro transfection of marrow stromal stem cells (also referred to as mesenchymal stem cells, MSCs) in monolayer with plasmid …
-
Characterization of Bioreducible Poly(B-amino Ester) Nanoparticles for siRNA Delivery
… siRNA release system to increase safety and transfection efficacy. We hypothesized that the addition of disulfides to the main backbone of PBAE NPs would reduce cytotoxicity and increase siRNA transfection efficacy. Glutathione, a reducing agent present in the cytosol, can reduce a disulfide …
Page 1 of 25