Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"transepithelial electrical resistance"”.
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Investigation of the absorption pathways of some poorly absorbed drugs using human intestinal (CAC0-2) cell monolayers
… they were used to elucidate and characterise the transepithelial transport of two protein kinase C inhibitors, N-(3-chlorophenyl)-4-[2-(3-hydroxypropylamino)-4-pyridyl]-2-pyrimidinamin (CHPP) and N-benzoyl-staurosporine (NBS), and the polypeptide, human calcitonin. Lanthanum ions are proposed as a …
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Exposure of 3D organotypic vaginal tissues to semen induces expression of the chemokine MCP-1, but does not alter tissue integrity
… and tissue integrity by histology and transepithelial electrical resistance (TEER) assessments. We used three multilayered differentiated EpiVaginal™ models: VEC-FT, which contained both epithelium and fibroblast layers; VEC-PT, which is comprised of differentiated epithelium only, and …
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A microphysiological system for studying barrier health of live tissues in real time
… electronics for acquiring and calculating transepithelial electrical resistance (TEER); and a scalable system architecture to allow multiple chambers running in parallel for increased throughput. The experimental results demonstrate that the system can maintain tissue viability for up to 72 …
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Role of matriptase in the regulation of epithelial barrier permeability studied using MDCK cells
… of! epithelial! function! with!measurements!of!transepithelial!electrical!resistance!(TEER).! Inhibitors!of!matriptase!proteolytic!activity!delayed!the!restoration!of!TEER!after! calciumNswitch! in!MDCKNI,! which! develop! high! TEER! and! lack! the! “leaky”! tight! …
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Differentiation of Normal and Cystic Fibrosis Human Lung Epithelial Cells in a Decellularized and Reconstituted Mouse Lung
… air liquid interface for 35 days as confirmed by transepithelial electrical resistance measurement, histology, Ussing chamber analysis, and immunofluorescence staining of differentiation factors. I also found that this conditional reprogramming method permits cloning of human bronchial epithelial …
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Effects of GABA on inflammation and intestinal barrier disruption
… increasing concentrations of GABA (0 – 10 mM). Transepithelial electrical resistance (TER) measurements and the flux of (FITC)-labeled dextran were quantified (0-48 hrs). Our results showed dose- and time-dependent effects of GABA exposure on monolayer integrity. Exposure of cells to 0.5 – 1 mM, …
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Investigating the impact of e-cigarettes on lung cells: a combined in vitro and computational study
… flavour. Biological responses were assessed via transepithelial electrical resistance (TEER), lactate dehydrogenase (LDH) release, IL-8 secretion, and gene expression. Nicotine salt and flavoured aerosols significantly reduced TEER, increased LDH and IL-8 levels, and downregulated OCLN, TJP1, …
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IN VITRO LUNG EPITHELIAL CELL TRANSPORT AND ANTI-INFLAMMATORY ACTIVITY FOR LIPOSOMAL CIPROFLOXACIN
… the highly restricted Calu-3 cell monolayers was transepithelial electrical resistance-independent, which suggested predominant transcellular transport. Compared to CPFX, Lipo-CPFX showed 6-18 times slower transport, while the flux was increased with increasing concentration proportionally without …
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Mechanism(s) involved in the transport of Fenretinide across Caco-2 monolayers
… it as a Povidone K 25 solid dispersion. The Transepithelial Electrical Resistance (TEER) and antipyrine permeability (transcellular marker) were not affected after treatment with 0.5% PVP K 25 on the apical side. The apparent permeability coefficient (P<sub>app</sub>) of fenreti nide was …
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Effects of Quaternary Ammonium Disinfectants on Mouse Reproductive Function
… a two-compartment culture system that measures transepithelial electrical resistance (TER). Sertoli cell cytotoxicity correlated with decreased TER at ADBAC+DDAC concentrations above 0.001%. In-vitro fertilization capacity of epididymal sperm was reduced in males given a 10-day rest period …
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Effect of Diabetes Mellitus on Ocular Surface Tight Junctions and Glycocalyx
… for 24 and 72 hours. Trans-epithelial electrical resistance and scratch assay were performed to quantify barrier functions and cell migration. Gene and protein expression of tight junction proteins: claudin-1, claudin-2, claudin-3, ZO-1, ZO-2, ZO-3, and occludin was quantified using …
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Neuroprotective effects of PACAP, VIP and NAP against hyperglycaemic retinal damage
… blood retinal barrier with permeability and transepithelial electrical resistance assay. The underlying signal transduction pathways activated by each peptide and the impact of hyperglicaemia on the expression and distribution at retinal levels of the inflammatory IL-1 mediators were also …
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Dietary carbohydrates influence the structure and function of the intestinal alpha-glucosidases
… of Caco-2 cell monolayers, which affect transepithelial transport of solutes permeating the cell monolayer barrier. A transepithelial electrical resistance experiment was conducted to measure the integrity of the Caco-2 monolayer in response to maltooligosaccharides. Maltose promoted …
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Markers and mechanisms of pre-eclampsia in pregnancies complicated by diabetes
… to 16 days, then exposed to N-LDL vs. HOG-LDL. Transepithelial electrical resistance (TEER) was measured after 6, 12 and 24 hours of treatment and fluorescein-isothiocyanate (FITC)-dextran leakage was measured after 24 hours of treatment. At termination of experiment, tight junction (zonula …
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The effects of green tea, green rooibos and their major flavonoids (EGCG and aspalathin) on testicular cell health in vitro
… lactate secretion and SCB integrity (by transepithelial electrical resistance). Within the tested concentration range of the teas and flavonoids used in this study, no cytotoxic effects were observed in Leydig or Sertoli cells. The experimental data further suggest that both green tea and …
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The effects of ethanol and aspalathus linearis on immortalized mouse brain endothelial cells (bEnd5)
… against housekeeping genes, GAPDH and HPRT. Transepithelial electrical resistance (TEER) was measured using the Ohm Millicell-electrical resistance system, while bEnd5 monolayer morphology was analysed using the Zeiss scanning electron microscope. Both concentrations of EtOH led to an overall …
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Development of in vitro infant intestinal models to assess responses to infant formulae
… High NaB concentrations (50–125 mM) reduced transepithelial electrical resistance (TEER) and increased paracellular permeability of Caco-2 monolayers in a dose-dependent manner under hyperosmotic conditions. NaB uptake occurred via monocarboxylate transporters (MCT-1, SMCT-1). However, NaB …
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Synergism between immune activators and progestogens: differential effects on gene expression and HIV-1 replication and the role of the glucocorticoid receptor
… was assessed by confocal microscopy and transepithelial electrical resistance measurements. For infection assays, TZM-bl cells were exposed to HIV-1Bal-Rellina infectious molecular clones (IMCs) before treatment progestogen alone or in combination with immune activators. In addition, …
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Study of apoptotic deletion mediated by Bifidobacterium longum with construction of recombinant strains for Serpin encoding gene and phenotypes comparison in a pig cell model
… by MTT cell proliferation assay were tested too. Transepithelial electrical resistance (TER) of polarised PSI C1 was measured during 48 hours co-culture. TER, used to observe epithelium permeability, decrease during pathogenesis and tissue becomes permeable to ion passive flow lowering epithelial …
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Further elucidating the effects of ruminal and hindgut acidosis on the ruminant animal
… from a mesenteric catheter under anesthesia. Transepithelial electrical resistance (TEER) was determined in ileum, cecum, and colon in Ussing chambers. There was a treatment × time effect (P = 0.05) for cecal pH with HGA ewes having lesser cecal pH after d 1. A treatment × time interaction was …