Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 38 for “"transdifferentiation"”.
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Transdifferentiation of fibroblasts to neural stem cells
The developmental process is carefully controlled by transcriptional and epigenetic changes that occur as a zygote transforms into an adult organism. This process can be reversed by the overexpression of transcription factors Oct4, Sox2, Klf4, and c-Myc, which reprogram a differentiated cell!s …
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Investigating neuroectodermal transdifferentiation: How do CNS progenitors form Schwann cells?
Myelin sheaths provide trophic support to axons, allow rapid nerve impulse conduction and are critical for neuronal function. Under homeostatic conditions, axons of the central nervous system (CNS) are myelinated by oligodendrocytes. However, in response to demyelination, Schwann cells (SCs), the …
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ENHANCING CARDIAC TRANSDIFFERENTIATION OF FIBROBLASTS USING SINGLE-CELL TRANSCRIPTOMICS ANALYSIS AND HIGH-THROUGHPUT CHEMICAL LIBRARY SCREENING APPOACHES
Cardiac transdifferentiation could potentially be used to treat acute myocardial infarction, but it is hampered by the lack of effective experimental protocols. By performing high-throughput chemical library screening, we serendipitously discovered that dimethyl sulfoxide (DMSO) itself improves the …
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Small Molecules Modulate Chromatin Accessibility to Promote NEUROG2-Mediated Fibroblast-to-Neuron Reprogramming
… factors is sufficient to induce the transdifferentiation of lineage-restricted fibroblasts into functional neurons; however, a mechanistic model of the immediate-early events that catalyze this conversion has not been well defined. We utilized a high-efficiency reprogramming system of …
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Elucidating the regulatory mechanisms of pterygium
… and Western blot were utilized to detect cell transdifferentiation. Human angiogenesis protein array was used for investigating angiogenic activities in pterygial fibroblasts. Gene microarray was employed to provide a global profile of gene expression in unstimulated and treated (1ng/ml TGFβ2 …
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Molecular Control of Lens Development in Xenopus Laevis
… lens-forming process termed cornea-lens transdifferentiation. Considering that both lens-forming processes result in the same ultimate outcome, it begs the question: How related are the molecular events involved in the embryonic and regenerative processes? Chapter 2 will examine gene …
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Fibroblasts: Key Cells in Inflammation and Fibrosis
… that promotes fibroblast to myofibroblast transdifferentiation. We hypothesized that TGFb induces myofibroblast transdifferentiation via activation of fibroblast-RelA, an event that may precede the development of vascular inflammation and AAA formation. Using primary human fibroblasts, I …
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Defining metabolic zonation as a determinant of hepatocyte plasticity
… that mature hepatocytes have the capacity for transdifferentiation in order to contribute to biliary repair; however, it remains unknown whether all hepatocytes possess this propensity or whether lineage plasticity is zonated within the lobule. This study aimed to investigate whether the …
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Identification and Classification of Novel Gene Products Involved in Development and Regeneration in Xenopus Laevis
A cornea-lens transdifferentiation-specific cDNA library has been used to characterize new molecular events in lens regeneration. Most of these genes are also expressed during lens development, illustrating the similarity between mechanisms of development and regeneration. A subset of genes; whose …
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Epithelial cell plasticity in chronic liver disease (MASLD/NAFLD)
… 2020). A potential mechanism of interest is the transdifferentiation of cholangiocytes into hepatocytes via either direct differentiation or via a progenitor cell population (Michalopoulos & Khan, 2015). However, the reality of this process in a disease context especially in human remains to be …
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Role of Macrophages in Ocular Surface Fibrosis
… and whether macrophages could cause the transdifferentiation of ocular surface fibroblasts into myofibroblasts, thus contributing to oGVHD-associated fibrosis.</p> <p>Bone marrow cells were cultured in M-CSF to obtain M0 macrophages, which were subsequently polarized into M1 and M2 …
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Molecular Profiling: Gene Expression Reveals Discrete Phases of Lens Induction and Development in Xenopus Laevis
… characterize their roles in development and transdifferentiation. The matrix metalloproteinase Xmmp-9 was found to function during migratory and morphogenetic processes during development, and it was found to be upregulated during wound healing. Psf2 codes for a factor which functions during …
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Fate plasticity and reprogramming in genetically distinct populations of Danio leucophores
… pigment cells—leucophores—one of which arises by transdifferentiation of adult melanophores and another of which develops from a yellow–orange xanthophore or xanthophore-like progenitor. Single-cell transcriptomic, mutational, chemical, and ultrastructural analyses of zebrafish leucophores …
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The role of nerve growth factor and p75 neurotrophin receptor in recovery from liver fibrosis
… in vivo without preventing hepatic stellate cell transdifferentiation. Moreover, in recovery from experimental liver fibrosis the fall in pro-nerve growth factor mirrors loss of hepatic myofibroblasts by apoptosis. Thus, nerve growth factor species have a differential effect on hepatic …
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Cochlear hair cell regeneration from neonatal mouse supporting cells
… cells are generated primarily through direct transdifferentiation of supporting cells, although a small number show evidence of proliferation. Inner pillar cells show the greatest transdifferentation capability, giving rise to immature outer hair cells, and transdifferentiating in response to …
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Targeting Endothelial Pathological Calcium Signaling Accelerates Resolution of SARS-CoV-2-Induced ARDS
… by accelerating alveolar type II to type I cell transdifferentiation, contributing to restoration of the alveolar-capillary barrier. Collectively, these findings identify EB3 as a critical upstream regulator of endothelial dysfunction in ARDS and establish its inhibition as a novel therapeutic …
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Identification of Unique Hematopoietic Stem Cell Properties
… generate high levels of cardiomyocytes through transdifferentiation. In contrast, low frequency HSC-derived cardiomyocytes were observed outside the infarcted myocardium, but were exclusively derived through cell fusion. Although such heterotypic cell fusion with hematopoietic cells and …
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Consequences of Plk4 overexpression in mouse pancreatic organoids
… RT-qPCR analyses revealed ductal-to-endocrine transdifferentiation, with insulin secretion and expression of β-cell markers in Plk4OE +dox organoids. I then used whole-transcriptome sequencing to investigate the consequences of Plk4 overexpression on gene expression. Results revealed …
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Tracing clonal cell plasticity in the microenvironment of pancreatic ductal adenocarcinoma
… strategy for investigating the clonality of transdifferentiation in the microenvironment of pancreatic cancer.
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