Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 58 for “"transcriptomic profiling"”.
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Transcriptomic profiling of macrophage polarization
… most popularly used platforms for single-cell transcriptomics. I then focus on my work characterizing macrophage polarization and repolarization in vitro, where I performed deep transcriptomic profiling at high temporal resolution as macrophages were polarized with cytokines that drive them …
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Transcriptomic Profiling of Hypothalamic Kiss1 Neurons
… and AVPV Kiss1 (Kiss1AVPV) neurons, unbiased transcriptomic profiling was done using RNA-sequencing on pools of Kiss1 neurons obtained by enzymatic dissociation of brain tissue. Principal component analysis revealed a clear clustering of Kiss1AVPV neurons from the Kiss1ARC neurons, while …
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Transcriptomic profiling of human cells destined for therapeutic applications
… chapter describes my characterization of the transcriptomic profiles of BMAC from OA patients and healthy donors. The results suggest overexpression of immune related pathways in BMAC samples from OA patients compared to samples from non-OA donors. Since pro-inflammatory cytokines play a role …
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Characterization and transcriptomic profiling of Arabidopsis thaliana under cellulose biosynthesis inhibitor treatment
… a protein with seven-membrane spanning helices. Transcriptomic profiling of Arabidopsis thaliana in response to CBI treatment revealed differential expression of genes related to membrane dynamics, stress responses, cellular transporters, and general metabolism. Processes containing CESA …
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Development of advanced methods for large-scale transcriptomic profiling and application to screening of metabolism disrupting compounds
High-throughput transcriptomic profiling has become a ubiquitous tool to assay an organism transcriptome and to characterize gene expression patterns in different cellular states or disease conditions, as well as in response to molecular and pharmacologic perturbations. Refinements to data …
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Understanding regional diversity in the human biliary tree through transcriptomic profiling of primary tissues and in vitro derived organoids
The biliary tree is a series of ductular tissues responsible for the drainage of bile produced by the liver and pancreatic secretions from the pancreas. The biliary tree is affected by a diversity of life- threatening diseases collectively called cholangiopathies. Cholangiopathies show …
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Analysis of cellular responses to microwave irradiation in E. coli and change of oxygen level and culture medium in human cancer cell lines using RNA-seq based transcriptomic profiling
… gene expression (DGE) analysis at the transcriptomic level as its primary objective. Among the NGS technologies, the Illumina platforms are the current standard for RNA-seq analysis for their best cost efficiency and sequencing accuracy. In this study, we employed Illumina-based RNA-seq …
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Characterization of genetically distinct neuronal subtypes in the lateral habenula using Cre mouse lines
… and serotonergic pathways. Recent single-cell transcriptomic profiling of the LHb has revealed that it consists of four neuronal subtypes that are localized into four subregions. These have been designated as the HbX, lateral, oval/medial, and marginal subregions of the LHb. Each subregion is …
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Integrative Analysis of Omics Data In Adult Glioma and Other Tcga Cancers to Guide Precision Medicine
<p>Transcriptomic profiling and gene expression signatures have been widely applied as effective approaches for enhancing the molecular classification, diagnosis, prognosis or prediction of therapeutic response towards personalized therapy for cancer patients. Thanks to modern genome-wide profiling …
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Identification of therapeutic targets and dependencies in ZNF703-amplified breast cancer
… on homologous recombination. Interactomic and transcriptomic profiling suggests ZNF703 binds to transcriptional repressors and interferon-related transcription factors, resulting in downregulation of interferon response. Notably, underexpression of interferon response genes is associated with …
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SKIN COMMENSAL STAPHYLOCOCCUS PROMOTES SYSTEMIC V-GAMMA-6 GAMMA-DELTA T CELL RECONFIGURATION
… signaling and the endogenous microbiota. Transcriptomic profiling reveals a transitional state in dermal Vγ6⁺ γδT cells from skin colonized mice, marked by changes in tissue-residency and migration-associated genes. Lymphatic trafficking, proliferation and long-term recirculation sustain …
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Profiling, prototyping, and perturbing human immune responses
… one which enables cell-type specific transcriptomic profiling of human blood using an integrated fluidic circuit, and another which enables combinatorial chemical perturbation of human immune cells using droplet microfluidics. In addition, we apply this framework to identify immune …
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Genesis of the human germline
… these questions, I performed highly-resolved transcriptomic profiling and careful epigenomic characterisation of cis-regulatory regions at key steps during the in vitro model. To determine the regulation of the transient gain and loss of competence for germ cell fate, I re-engineered a CRISPR …
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Harnessing the Regenerative Characteristics of Meniscus Superficial Zone Cells
… superficial layer cells on multiple scales using transcriptomic profiling, migration, and proliferation assays, mechanosensation analysis, and traction force microscopy. After, the matrix deposition potential of superficial cells was assessed on electrospun nanofibers. Here, we show at the …
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Functional analysis of amyotrophic lateral sclerosis mutations using Drosophila cell culture models
… biology, high-throughput genetic screening, and transcriptomics analysis to identify potential therapeutic targets of ALS. Transient Drosophila S2R+ cell models expressing wild-type or mutant forms of the ALS-associated genes FUS, SOD1, TDP43 were generated to evaluate protein expression, …
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Oncogene-driven post-transcriptional regulation in lung cancer
… KRAS and RIT1 cells. Quantitative proteomic and transcriptomic profiling revealed that both mutant KRAS and mutant RIT1 promoted canonical Ras signaling, and overexpression of wild-type RIT1 resulted in phenotypes similar to those of oncogenic RIT1 and KRAS, including induction of …
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Qki-Mediated Cholesterol Biosynthesis In Eye Lens and Myelin of The Central Nervous System
… activator of cholesterol biosynthesis. Using transcriptomic profiling, we found cholesterol biosynthesis was the most downregulated pathway in Qki-depleted lens and brain. Indeed, mRNA and protein levels of cholesterol biosynthesis genes were reduced in Qki-depleted lens cells and …
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Qki-Mediated Cholesterol Biosynthesis In Eye Lens and Myelin of The Central Nervous System
… activator of cholesterol biosynthesis. Using transcriptomic profiling, we found cholesterol biosynthesis was the most downregulated pathway in Qki-depleted lens and brain. Indeed, mRNA and protein levels of cholesterol biosynthesis genes were reduced in Qki-depleted lens cells and …
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Organ-Level Communication During Heart Regeneration In Zebrafish.
… a transcription factor gene, cebpd, through transcriptomic profiling of the uninjured brain and kidney during zebrafish heart regeneration. The expression of cebpd is induced both locally in the epicardial tissue of regenerating hearts and distantly in the brain ependymal layer and renal …
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The impact of a single nucleotide polymorphism in fusA1 on biofilm formation and virulence in Pseudomonas aeruginosa
… comprising an acute infection. Proteomic and transcriptomic profiling of the mutant strain provided an insight into the genetic basis behind these phenotypes, identifying the up-regulation of multidrug efflux systems and modulations to the chemotactic systems. This study also found links …
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