Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 92 for “"transcriptional repression"”.
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MECHANISM OF TRANSCRIPTIONAL REPRESSION OF MMP-9 BY INTERFERON-β
… 13-acetate (PMA)-induced MMP-9 expression at the transcriptional level, the molecular mechanism of this repression is poorly understood. We describe here a novel mechanism for repression of MMP-9 transcription by IFN-beta in HT1080 fibrosarcoma cells. Using reporter assays with promoter deletion …
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AN INVESTIGATION OF THE MECHANISMS OF GROUCHO MEDIATED TRANSCRIPTIONAL REPRESSION
… the mechanism underpinning Gro-mediated transcriptional repression. Recent studies that used Gro ChIP-seq data from Drosophila cells and embryos indicated that Gro could mediate transcriptional repression by promoting Pol II promoter-proximal pausing. In this work, I took three different …
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Characterizing the Role of SUMO in Transcriptional Repression in S. cerevisiae
… Additionally, SUMO has been widely implicated in transcriptional regulation and chromatin organization, although the nature of its role remains unclear. This thesis aims to: 1) complete a comprehensive characterization of the SSR, and 2) elucidate the true role of SUMO in transcriptional …
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Novel Mechanisms of Transcriptional Repression by the Paired-like Homeodomain Transcription Factor Goosecoid
… level, Gsc has been described to function as a transcriptional repressor by directly binding to paired homedomain binding sites on target promoters. Here, I describe a novel mechanism of transcriptional repression by Gsc. Using a molecular and embryological approach, I demonstrate that the …
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Caracterización funcional de Snail2 como represor de cadherina- E y como regulador del proceso de carcinogenésis química
… cells of epithelial origin (carcinomas). The transcriptional repression is one of the main mechanisms of negative regulation of E-cadherin expression in both embryonic development and during tumor progression. In recent years there has been identified different transcriptional repressors of …
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EPIGENETIC MECHANISMS OF POLYCOMB REPRESSIVE COMPLEX 1 IN INTESTINAL LINEAGE COMMITMENT
Polycomb group proteins are key transcriptional repressive complexes involved in cell fate decisions during development and cell identity maintenance during homeostasis (1). Polycomb group proteins primarily assemble into two different complexes: Polycomb repressive complex 2 (PRC2) with the …
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The Role of DNA Methylation and Methyl Binding Domain Protein 2 in the Regulation of Human Embryonic and Fetal Beta Type Globin Genes
… and methyl binding domain protein 2 in the transcriptional regulation of the human embryonic and fetal beta type globin genes. Adult mice containing the entire human β-globin locus as a yeast artificial chromosome (βYAC) express very low levels of the fetal γ-globin gene. However, treatment …
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The synthetic multivulva genes and their suppressors regulate opposing cell fates through chromatin remodeling
… The class B and C genes encode presumptive transcriptional repressors and chromatin-remodeling factors. The synMuv genes likely repress the transcription of the Ras pathway ligand lin-3 EGF. Some class B synMuv proteins are homologs of a Nucleosome Remodeling and Deacetylase (NuRD)-like …
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The association of the secondary DNA-binding site of linker histone H5 in a nucleosome
… 30-run chromatin fibre as well as their role in transcriptional repression, it is essential to know their location on the nucleosome. In this study, we have modelled the location of the globular domain of chicken linker histone HS (GHS) on the nuc1eosome. The primary DNA binding site of GH5 was …
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Mechanisms of molecular switching in the Wnt signal transduction pathway
… it access to target genes and relieving their transcriptional repression by Gro/TLE. Many of the components of these complexes have now been identified, but details of their regulation, and in particular the mechanisms by which they are switched on and off, remain poorly understood. The …
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C. Elegans OMA-1 and OMA-2 are Transcriptional and Translational Repressors of Germline Fate
… any molecular functions. My thesis demonstrates transcriptional repression function of OMA proteins in newly fertilized embryos and translational repression functions during oogenesis. I showed that OMA-1/2 are redundantly required for global transcriptional repression before the onset of zygotic …
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Identification of Factors Involved In Dna Methylation of Cpg-Island-Promoters
<p>Repression of many tumor suppressor genes (TSGs) in cancer is mediated by aberrantly increased DNA methylation levels at promoter CpG islands (CGI). About one-fourth of empirically defined human promoters are surrounded by or contain clustered repetitive elements. It was previously observed that …
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CHD Chromatin Remodelers Promote Epigenetic Control of Development in Arabidopsis thaliana
… process that facilitates activation and repression of gene expression as well as compaction of large eukaryotic genomes into compact nuclei. The CHD3 family of chromatin remodelers play key roles as epigenetic modifiers and transcriptional regulators in both plants and animals. PKL is a …
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Repression Of Autophagy During Erythroid Development By Gfi1b
… The laboratory recently discovered that the transcriptional repressor GFI1B (growth factor independence 1b) is a critical regulator of autophagy. The role of GFI1B in inhibiting autophagy was initially indicated by chromatin immunoprecipitation screens which uncovered several mediators of …
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MeCP2 and the Epigenetic Regulation of Excitatory Synaptic Transmission
… transcription. Mutations in one such gene, the transcriptional repressor methyl-CpG-binding protein 2 (MeCP2), lead to a form of mental retardation called Rett Syndrome (RTT). Though the MeCP2 protein is expressed ubiquitously, symptoms of RTT patients are primarily neurological, which include …
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Defining the essential regulators of naive human pluripotent stem cell reprogramming
… during reprogramming and these genes become transcriptionally repressed as cells successfully reprogramme, suggesting a key gene silencing role for PRC1.3. Deletion of PRC1.3 reduces the global abundance of the PRC1-deposited H2AK119ub1 modification, and levels of PRC2-deposited H3K27me3 …
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Induction and characterization of de novo methylation by benzo[A]pyrene in the cancer cell lines MCF-7 and MDA-MB-231
… that de novo methylation is a consequence of transcriptional inactivity. Benzo[a]pyrene can contribute to transcriptional repression by sequestering the transcription factor, Spl. To test this hypothesis in our system, MCF-7 cells were transiently transfected with a reporter construct …
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De novo designs of programmable toehold-mediated transcriptional attenuators
… are frequently utilized as translational and transcriptional regulatory elements to construct a diverse array of biological devices. RNA-only genetic circuitry provides several advantages over other types of genetic circuitry. While numerous transcriptional activation systems exist, there is a …
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Genetic characterization of a family of mouse 5-methylcytosine binding protein genes
Cytosine-5-methylation has been associated with repression of transcription across eukaryotic phyla. In mammals, defects in methylation are associated with disease, developmental defects, and lethality. Furthermore, methylcytosine-specific binding proteins have been described which seem to effect …
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RNA-based therapeutic approaches for FTDP-17
… RNA (siRNA) approach to modulate post-transcriptional gene silencing of specific isoform associated to FTDP-17. CHAPTER 4 employs long non coding RNA (lncRNA) to mediate post transcriptional repression of tau protein associated to FTDP-17 and deciphers its auxiliary role in splicing of …
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