Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"transcriptional reporter"”.
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Tuning DNA binding and gene expression using zinc finger proteins and engineered promoters
… project, we worked to develop a new system for transcriptional control based on tunable synthetic TFs, which are designed to yield programmable linear responses in gene expression. To accomplish this, we used zinc finger proteins (ZFPs) as regulators of engineered promoters assayed by green …
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Characterization of lin-42/period transcriptional regulation by the Ikaros/hunchback-family transcription factor ZTF-16 in Caenorhabditis elegans
… ZTF-16 through phenotypic assays and analysis of transcriptional reporter strains. Our data show that ZTF-16 regulates the cyclic expression of lin-42A and lin-42B during larval development. However, ztf-16 is not expressed during the adult stage and does not regulate lin-42C, which is expressed …
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Functional analysis of the Argonaute family proteins during early embryonic and germ cell development in the mouse
… soon after birth. I generated a Miwi2tdTomato transcriptional reporter allele and proved that Miwi2 expression marks a subpopulation of spermatogonial cells that possesses surface markers, cell cycle and gene expression profile reminiscent of stem cells in juvenile testis. Comparison of …
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Regulation of C-type lectin-like receptors dectin-1 and CLEC-2 by tetraspanins
… a nuclear factor of activated T-cells (NFAT) transcriptional reporter assay in the Jurkat T-cell line, transient over-expression of CD37 was found to powerfully inhibit dectin-1 signalling following stimulation with its ligand, β-glucan. Over-expression of other tetraspanins also inhibited …
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A transthyretin-related gene and a neuroligin-like gene prevent 6-hydroxydopamine-induced dopaminergic neurodegeneration in <i>C. elegans</i>
… dopamine signalling. A P<i>glit-1</i>::GFP transcriptional reporter is expressed in the pharynx, intestine and possibly dopaminergic neurons. The second isolated mutant <i>ttr-33</i> in contrast does not display dopamine-related behavioural defects. TTR-33 is likely secreted from the …
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Transcriptional Regulation of the MGA Virulence Regulon in Streptococcus Pyogenes
… (flesh-eating disease). The GAS possesses a transcriptional regulator called Mga, or the multiple gene regulator of the GAS, that responds to growth phase and environmental signals to activate virulence genes necessary for colonization, internalization, and immune evasion of the bacterium. In …
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The Influence of The Pep-Pts and Other Metabolic Systems On The Master Virulence Regulator Atxa and to xin Gene Expression In Bacillus Anthracis
… and activity of the protein. PCVRs are transcriptional regulators, named for the regulatory domains subject to phosphorylation by the phosphoenolpyruvate phosphotransferase system (PEP-PTS), termed PTS Regulatory Domains (PRD), and for their impact on virulence gene expression. …
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Identification of novel protein kinase regulators of TGFβ signalling
… screen using an endogenous TGFβ-dependent transcriptional reporter cell line in order to identify potential novel regulatory components of the TGFβ signalling pathway. From this screen and subsequent validation experiments, I observed that three structurally unrelated small-molecule …