Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"transcriptional noise"”.
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Comparison of dispersion metrics for estimating transcriptional noise in single-cell RNA-seq data and applications to cardiomyocyte biology
… The quantification and analysis of transcriptional variability poses an opportunity to study transcription and gene regulation from a new angle. Transcriptional variability has already been implicated in a number of biological processes, including in immune system development and in …
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Computational Analysis of Transcriptional Regulation
… organisation of eukaryotic genomes and the post-transcriptional control of RNA fates. Eukaryotic nuclear DNA is tightly packaged as chromatin that is further folded into higher order structures. The 3D folding of eukaryotic genome lends itself to the formation of interactions between otherwise …
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Quantifying expression variability in single-cell RNA sequencing data
Transcriptional noise is an intrinsic feature of cell populations and plays a driving role in mammalian development, tissue homoeostasis and immune function. While expression heterogeneity, a phenotypic readout of transcriptional noise, has been broadly studied in prokaryotic model systems or by …
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The physics of cell-fate choice
… choice. Next, I present two models concerning noise in cell-fate choice--- a mechanical model of DNA supercoiling-mediated transcriptional noise and a coarse-grained model of noise in partitioning during cell division that can create and maintain a phenotypically heterogeneous population. …
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Statistical mechanical modeling of eukaryotic gene regulation
… by gene regulatory networks. Central to transcriptional regulation of gene expression is the regulation of the quantities of transcription factors (TFs) bound to genomic regulatory sequences. This thesis work is built on statistical mechanics to study the stochastic interactions of TFs …
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Single cell -omic landscapes in normal and perturbed haematopoiesis
… early lineage choice. Flowcytometric sorting and transcriptional or functional profiling of more mature oligopotent or unipotent bone marrow constituents has elucidated the later processes in the maturation of multiple haematopoietic lineages. The primary aim of this work was to generate an …
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Annotating and characterizing orphan gene in Zea mays via diverse RNA-Seq data
… transcribed but unannotated transcripts as transcriptional "noise", which I refer to "dark transcriptome". Some functional genes have been identified from this dark transcriptome. Most genes in the dark transcriptome are orphan genes. Orphan genes are the recently emerged young genes, which …
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Biophysical Analysis of S100A12 and the Nucleosome Core Particle
… E76K likely leads to enhancer hyperpriming and transcriptional noise, while R92T likely enables excessive chromatin mobility and epigenetic instability. Our analysis redefines key thermodynamic transitions in the NCP dissociation pathway and provides a unified model for how histone mutations …
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Date with Destiny: Genetic and Epigenetic Factors in Cell Fate Decisions in Populations of Multipotent Stem Cells
… results in a decline in the gene expression noise strength contributed by the promoter activation operation, which suggests that when ESCs become lineage-primed their transcriptional noise is constrained. In the bipotent model, the NMPs are identified as cells coexpressing Sox2 and …
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Assessing the transition state model for cellular differentiation in vivo: a case study of zebrafish neuromesodermal progenitors
… an increase in the critical transition index and transcriptional noise in the NMp population relative to its derivatives. Next, to follow the expression of neural and mesodermal markers within the tailbud at 18ss, 24ss and 28ss, I co-developed an image registration pipeline with my colleague in …
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A Structural Perspective on Bacterial Transcription
… analogous ATPases acting on PTCs to suppress transcriptional noise and R-loop formation may be widespread. These results hold significance for the bacterial transcription cycle and highlight a role for RapA in maintaining genome stability. Overall, these studies come together to provide a …