Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 47 for “"transcriptional networks"”.
-
Communication between layers in biological transcriptional networks
… cell. Much of the current work in modeling yeast transcriptional networks focuses on the regulatory effect of a class of proteins known as transcription factors (TF). However, other sets of factors also influence transcription, including histone modifications and states (HS), histone modifiers …
-
Expression-based reverse engineering of plant transcriptional networks
… in order to reverse-engineer the cellular transcriptional networks using gene expression data as a compass. This kind of studies has been considerably assisted in the recent years by the development of high-throughput transcript measurement platforms, specifically gene microarrays and …
-
TRANSCRIPTIONAL NETWORKS VARIATIONS DURING CELL CYCLE PROGRESSION IN HUMAN EMBRYONIC STEM CELLS
… cycle regulator Cyclin D appears to control the transcriptional activity of Activin/Nodal signalling during progression of the cell cycle in human Embryonic Stem Cells (hESCs). As a consequence, hESCs can only differentiate into endoderm in the Early G1 phase when Cyclin Ds are expressed at low …
-
Quantitative dynamic modeling of transcriptional networks of embryonic stem cells using integrated framework of Pareto optimality and energy balance
… and mass balance constraints. Analysis of transcriptional regulatory networks and metabolic networks in ESCs thus requires both a nonequilibrium thermodynamic and mass balance framework for designing and understanding complex ESC network approach as well as an optimality approach which can …
-
Practical Approaches to Biological Network Discovery
… biology, which is to identify the structure of a transcriptional network from high-throughput experimental data. Understanding of the connectivity of a transcriptional network is an important piece of the puzzle, which relates the genotype of an organism to its phenotypes. An overwhelming number …
-
Exploring flowering gene networks in soybean and arabidopsis through transcriptome analysis
… of this thesis was to explore the flowering gene networks in soybean through an RNASeq based transcriptome analysis. Chapter One discusses the effect of three photoperiod treatments, short day (SD), long day (LD) and shift from 3 weeks LD to 1 week SD on six domesticated varieties of Glycine max; …
-
A KLF6-driven transcriptional network links lipid homeostasis and tumour growth in clear cell renal cell carcinoma
… ccRCC. Thus, the identification of cellular networks on which ccRCC cells are highly dependent would facilitate the development of better diagnostic and/or therapeutic approaches for ccRCC. Super enhancers have been reported to drive the expression of critical transcription regulators in …
-
Uncovering a novel role for FXR-SHP axis in liver physiology, diseases and beyond
… is nuclear receptor signaling, which in turn can transcriptionally regulate gene networks. My doctoral thesis focuses on studying the role of two nuclear receptors, FXR and SHP in maintaining liver function. Farnesoid X Receptor (FXR) and Small Heterodimer Partner (SHP) are well-known regulators …
-
KINETIC ANALYSIS OF AUXIN-INDUCED TRANSCRIPTION FACTOR NETWORKS CONTROLLING LATERAL ROOT DEVELOPMENT IN ARABIDOPSIS THALIANA
… lateral root development through complex transcriptional networks. An 8-timepoint microarray of roots treated with indole-3-acetic acid (IAA), the major form of auxin, was utilized to identify the transcriptome wide kinetics of auxin response. This microarray allowed identification of …
-
Origins and Development of the Embryonic Vascular System in Xenopus
… more precisely the cell-signaling pathways and transcriptional networks that guide their fate. This work leads us to conclude the following: (1) blood island precursor cells in the Xenopus embryo can give rise to either blood or endothelial cells, and it is BMP-mediated activation of the …
-
FUNCTIONAL ROLE OF NON-CODING REGULATORY VARIANTS IN NEUROBLASTOMA
… regions and ultimately affecting gene expression networks. However, their interpretation is challenging. In our recent work, whole-genome sequencing (WGS) data from 151 neuroblastoma primary tumours identified two clusters of non-coding Single Nucleotide Variants (SNVs) in two distinct …
-
Genetic and Computational Tools for Engineering Complex Prokaryotic Systems
… of their gene regulatory and metabolic networks presents significant challenges for engineering efficient biosynthetic pathways. While CRISPR-Cas technologies have revolutionized genome editing in eukaryotes, the development of programmable, multi-layer transcriptional control in …
-
Scalable platforms for computation and memory in living cells
… (DRIVE - Chapter 6), tunable and multifunctional transcriptional factors for gene regulation in eukaryotes (crisprTF - Chapter 7), and an unbiased, high-throughput and combinatorial strategy for perturbing transcriptional networks for genetic screening (PRISM - Chapter 8). I envision the platforms …
-
MOLECULAR PROFILING OF UROTHELIAL CARCINOMA
… characterize urothelial carcinoma (UC) at the transcriptional level using gene expression microarrays to improve the classification and pathogenetic understanding of this disease. In the first two studies (Articles I and II), gene expression profiling was used to study dysregulated …
-
Principles for the design and construction of synthetic circuits utilizing protein-protein interactions in Saccharomyces cerevisiae
… biology, significant progress in creating networks using transcriptional and translational control has been made, but a network comprised solely of protein-protein interactions has not yet been built. To realize this goal, new design rules for assembling and connecting protein devices into …
-
Transposable elements and the regulatory logic of hematopoietic differentiation
… that TEs in the human genome contribute to the transcriptional networks regulating lymphoid cells. De-repression of TEs in hematopoietic stem cells, enacted via modulation of TE chromatin silencing machinery, facilitates the development of natural killer (NK) cells during lymphoid …
-
H-NS Counter-silencing as a Primary Regulatory Mechanism of Curli Gene Expression in Salmonella Typhimurium
Incorporation of horizontally acquired genes into transcriptional networks is essential for regulated expression of virulence in the bacterial pathogen Salmonella enterica serovar Typhimurium. The nucleoid-associated protein H-NS is able to recognize and bind AT-rich horizontally acquired genes, …
-
Impact of Stochastic Transcriptional Delay on Gene Networks
… for these reactions in models of gene regulatory networks is to incorporate dynamical delay. The stochastic nature of the reactions necessary to produce protein leads to a waiting time that is randomly distributed, complicating simulation and analysis. We examine this problem using different …
-
Identification and characterisation of HNF1B as a pro-tumorigenic lineage factor in clear cell renal cell carcinoma
… be fundamentally defined by tissue-specific core transcriptional networks. Thus, the aim of my PhD thesis was to identify novel TF dependencies in ccRCC, and characterise the mechanisms by which these essential TFs support these tumours. I performed a CRISPR/Cas9-based genetic depletion screen in …
-
The regulation of the oncogenic STAT transcription factors by the non receptor tyrosine kinase and Cdc42 effector ACK
… receptor in breast cancer, ACK regulates transcriptional networks in disease. In this work, new roles for ACK in transcription, mediated by its relationship with the signal transducer and activators of transcription (STAT) family, are uncovered. The STAT family are a 7-member family of …
Page 1 of 3