Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 153 for “"transcriptional activation"”.
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Eukaryotic Transcriptional Activation Mechanism: Protein-Protein Interactions
… I analyzed the function of distinct kinds of activation domains in yeast and human. The results showed that the proline-rich activation domain of CTF1 can, like acidic activation domains, activate transcription in yeast and human. Based on this, I compared the activation pathways by acidic and …
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Ctla-4 transcriptional activation: regulation of induced expression
… in effector T cells. The mechanisms driving transcriptional regulation of CTLA-4 are poorly understood. Our previous work identified a bona fide NFAT1 binding site in the proximal promoter for effector T cells. In addition, we found histone acetylation occurred after stimulation.</p> <p>As a …
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Regulation of SRC-3 localization and dynamics by phosphorylation during ERα-dependent transcriptional activation
Transcription is controlled by promoter-selective transcriptional factors (TFs), which bind to cis-regulatory enhancers elements, termed hormone response elements (HREs), in a specific subset of genes. Regulation by these factors involves either the recruitment of coactivators or corepressors and …
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Phenobarbital Induced Transcriptional Activation of CYP2B Genes by Chromatin Modification at the PBRU Region
… of CYP2B genes in the liver. PB-mediated CYP2B activation is regulated by the PB-dependent enhancer (PBRU) which contains binding sites for NF-1 and two nuclear receptor binding sites (NR-1,2). A third NR site is located at the 5' side of the PBRU. PB induces the nuclear translocation of a …
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Changes in large-scale chromatin structure and dynamics of BAC transgenes during transcriptional activation
Item marked as restricted to the 'UIUC Users [automated]' Group (id=2) by William Ingram (wingram2@illinois.edu) on 2010-05-14T20:52:51Z Item is restricted until 2012-05-14T20:52:43Z
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Amino Acid Residues in LuxR Critical for its Mechanism of Transcriptional Activation during Quorum Sensing
… understand the mechanism of LuxR-dependent transcriptional activation of the <I>lux</I> operon, PCR-based site-directed mutagenesis procedures have been used to generate alanine-substitution mutants in the C-terminal forty-one amino acid residues of LuxR, a region that has been hypothesized …
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LDB-1 AND SWI/SNF CHROMATIN REMODELING COMPLEX PARTICIPATE IN THE TRANSCRIPTIONAL ACTIVATION BY C. ELEGANS BLIMP1/PRDM1
… the human zinc finger and SET domain-containing transcriptional repressor BLIMP1/PRDM1. BLIMP1/PRDM1 and its orthologs are well studied as transcriptional repressors in many species including humans and mice. Interestingly, we found that in C. elegans, BLMP-1 acts as a direct transcriptional …
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Characterizing interactions between cAMP responsive element binding protein 1 and methyl-CpG-binding protein 2 as a potential transcriptional activation complex
Transcriptional activity is controlled by many types of DNA binding proteins. In addition to transcription factors that activate transcription by recruitment of RNA polymerase II, there are proteins like methyl-CpG-binding protein 2 (MeCP2). MeCP2 regulates transcription by binding to methylated …
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Role of region 4 of the sigma 70 subunit of RNA polymerase in transcriptional activation of the lux operon during quorum sensing
… Vibrio fischeri. The cell-density dependent activation of the luminescence (lux) genes of V. fischeri relies on the formation of a complex between the autoinducer molecule, N-(3-oxohexanoyl) homoserine lactone, and the autoinducer-dependent transcriptional activator LuxR. LuxR, a 250 amino …
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Role of the C-terminal domain of the <font face = "symbol">a</font> subunit of RNA polymerase in transcriptional activation of the <i>lux</i> operon during quorum sensing
… = "symbol">a</font>CTD of RNAP in LuxR-dependent transcriptional activation of the <i>lux</i> operon promoter has been investigated. The effect of seventy alanine substitution variants of the <font face = "symbol">a</font> subunit was determined <i>in vivo</i> by measuring the rate of …
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Characterization of a Putative Activation Domain in the Hulk Gene Family
… FLC, FLM and MAF2. To investigate the putative transcriptional activation domain in the carboxyl-terminus of the HULKs, full-length HULKs and deletion constructs were 3-AT titrated in yeast-one hybrids. It was found that the transcriptional activity varied between both the full-length and …
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Targeting a Transcriptional Activator Induces Changes in Large-Scale Chromatin Structure and Nuclear Positioning
This study addresses the relationship between transcriptional activation and large-scale chromatin structure and nuclear positioning. We have used an artificial system to target large amounts of the very strong viral transcriptional activator, VP16, to specific chromosome sites. The rationale of …
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Regulatory Mechanisms Controlling Expression of the Toll-Like Receptor 2 Subfamily
… subfamily members---one acting at the level of transcriptional activation, another at the post-transcriptional level, and finally one that regulates the post-translational localization of the receptor. In chapter five, I will summarize these findings and discuss the potential implications.
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Analysis of the interaction between the co-chaperone p23 and the aryl hydrocarbon receptor
… hydrocarbon receptor (AhR) carboxyl terminal transcriptional activation domain was cloned, purified in denatured conditions from bacteria, refolded via limited dialysis, and analyzed for proper refolding via co-immunoprecipitation with the known binding partner SRC-1. This AhR NΔ515 …
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Ire1 regulates cyp11a1 transcription to mediate steroidogenesis in the ovary
CYP11A1 transcriptional activation in response to gonadotropin hormones is critical to maintain fertility and support pregnancy. Therefore, we are interested in identifying factors that regulate CYP11A1 transcription. Our laboratory has identified several signaling proteins that activate CYP11A1 …
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Identifying Oncogenic Drivers of Poor Outcome in Triple Negative Breast Cancer
… effects of TGFβ2-pSMAD2/3 signalling towards transcriptional activation of EMT and metastasis driving genes. Furthermore, we identified this dysregulation occurs at the protein level with GLI2 requiring to interact with YWHAZ to partner with pSMAD2/3, to translocate to the nucleus where it act …
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The Role of TNRC6 in RNA Interference
Small RNAs can influence translation, splicing, transcriptional activation, and transcriptional repression, through the RNA interference (RNAi) pathway. TNRC6, also known as GW182, and Argonaute (AGO) are the core proteins of RNAi. TNRC6 is a scaffolding protein that associates with AGO and bridges …
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Functional Domains in the Multigene Regulator of the Group A Streptococcus
… mutant, previously identified to be deficient in transcriptional activation, as a guide to map functional residues within Mga. Electrophoretic mobility shift assays using purified proteins determined that the defect was not a result of the inability to bind DNA, so gain-of-function mutants were …
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Role of Synapsin In Long-Term Synaptic Facilitation In Aplysia
… was identified, raising the possibility that the transcriptional activator cAMP response element-binding protein-1 (CREB1) mediates the 5-HT-induced regulation of synapsin. Results from Chromatin Immunoprecipitation (ChIP) assays indicated that 5-HT treatment enhanced association of CREB1 …
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