Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"transcription units"”.
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Organization of RNA Transcription Units in Amphibian Oocytes
Finally, the mechanism of hnRNP-G recruitment to the lampbrush loops is investigated. Interestingly, the RNA binding domain of the protein is not required for recruitment specificity to the loops. Instead, a poorly characterized portion of the C-terminus of the protein is required for this process.
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Promoters and Heterogeneous 5' Termini of the Messenger RNAs of Adenovirus 2
… RNAs are transcribed from nine separate transcription units which function during early, intermediate and late stages of infection. I have sequenced capped 5' termini of messenger RNAs from each of these transcription units, and located the templates for each capped terminus in the …
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Isolation, characterization, and transcriptional autonomy of theout at first gene of Drosophila melanogaster
… promoter, and not with the promoters of adjacent transcription units, are poorly understood. To test if, and how, a transcription unit 3$\sp\prime$ of the enhancer rich decapentaplegic (dpp) disk region might remain transcriptionally autonomous of disk region influence, I isolated and …
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Acute Manipulation of Erna Level For Dissecting Its Roles In Transcriptional Regulation
… controlling cell-type and state specific transcription programs to dictate cell fates during development. Mechanistic understanding of enhancer action is important for both biology and disease research. In the human genome, more than 60k human enhancers were found to produce non-coding …
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Effects of Drought on Gene Expression in Maize Reproductive and Leaf Meristem Tissues as Revealed by Deep Sequencing
… previously known gene models and potential novel transcription units. An attempt was also made to exploit the data to understand the drought mediated transcriptional events (e.g. alternative splicing). Gene Ontology (GO) enrichment analysis revealed massive down-regulation of cell division and …
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Regulation of Gene Expression in Trypanosoma brucei
… II (RNA pol II) are arranged in polycistronic transcription units (PTUs). mRNAs are separated post-transcriptionally by coupled splicing and polyadenylation reactions. During the splicing reaction a 39 nt 'sliced-leader' is added to every mRNA, a process termed trans splicing. The arrangement …
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Engineering a Reverse C4 Photosynthetic Pathway in C3 species Arabidopsis thaliana
… large multigene constructs containing 9 and 10 transcription units. Homozygous lines carrying each construct were obtained and showed overexpression of most transgenes. Although plants carrying bundle sheath genes of the pathway showed no visible phenotypes and those carrying mesophyll genes …
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Protein factors and 5' flanking sequences involved in the expression of the mouse myelin basic protein gene.
… This gene complex consists of two overlapping transcription units, golli and MBP, which are regulated by two distinct promoters. The golli unit is expressed in cells of the oligodendrocyte lineage (Central Nervous System), neurons, B and T lymphocytes, testis and thymus. However, the MBP unit …
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Co-transcriptional recruitment of the U1 snRNP
… of most pre-mRNAs occurs, at least in part, co-transcriptionally. In order to validate this principle in yeast and establish an experimental system for monitoring spliceosome assembly in vivo, I have employed the chromatin immunoprecipitation (ChIP) assay to study co-transcriptional splicing …
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A molecular investigation of the novel gene underlying autosomal dominant retinitis pigmentosa in a South African family
… tool for the purpose of isolating genes or transcription units within the RP17 critical interval. In this regard, purified YAC DNA was isolated using pulsed-field gel electrophoresis and the cDNA selection technique was employed to generate a transcription map. This approach was applied to …
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Discrete elements of the U2 snRNP regulate its intranuclear trafficking and recruitment to nascent transcripts
In this study, I characterize the elements of the U2 snRNP that control its intranuclear trafficking and association with nascent transcripts. Through a mutational analysis, I demonstrate that stem loops 3 and 4 (SL3 and SL4) are both required for U2 snRNP association with nascent transcripts and …