Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 38 for “"transcription termination"”.
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Factors and mechanisms of archaeal transcription termination and DNA repair
… interactions with RNAP at all stages of the transcription cycle- initiation, elongation, and termination. While studies of transcription initiation and elongation have identified multiple regulatory transcription factors and defined mechanisms, only a handful of protein factors able to …
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Mechanism of transcription termination: Role of chromatin and R-loops
La transcripción llevada a cabo por la ARN polimerasa II (RNAPII) es un proceso fuertemente regulado que se compone de tres fases: iniciación, elongación y terminación. De todas ellas, la terminación es la etapa menos estudiada y comprendida, sin embargo, de ella dependen procesos esenciales como …
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An in vitro transcription termination system to analyze spinach chloroplast promoters
… RNA polymerase would accurately terminate transcription in vitro, the 3$\sp\prime$ end of the rbcL gene as well as various factor-independent transcription terminators from E. coli were fused to the spinach chloroplast rbcL promoter. Transcription of the rbcL minigene did not result in …
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Transcription termination by bacteriophage T7 RNA polymerase at rho-independent terminators
To investigate the mechanism of transcription termination by bacteriophage RNA polymerases, templates encoding variants of the transcription-termination structure in the regulatory region of the threonine (thr) operon of Escherichia coli were used. The thr terminator comprises two distinct …
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The molecular mechanisms of transcription termination and pausing in the Escherichia coli threonine operon regulatory region
To study the mechanism of transcription termination at a rho-independent terminator, homoduplex and heteroduplex templates that contained mutations in the G + C-rich region of dyad symmetry in the Escherichia coli thr operon attenuator were constructed. Assays of these templates in an in vitro …
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An analysis of the effect of mutations in the leader region of the threonine operon of Escherichia coli on attenuation or transcription termination
The expression of the threonine operon of Escherichia coli is controlled by attenuation. An open translational reading frame in the leader region preceding the structural genes contains 21 codons with eight threonine and four isoleucine codons. The model for attenuation proposes that regulation …
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Transcriptional Regulation of the Bacillus Subtilis pyrG Gene Encoding Cytidine Triphosphate Synthetase
… the formation of the terminator and permits transcription readthrough. When intracellular CTP levels are high, I propose the regulatory protein dissociates from the pyrG leader RNA thereby allowing terminator formation and transcription termination.
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INVESTIGATING THE BASES OF THE SPECIFICITY OF THE RESTRICTOR COMPLEX FOR THE TERMINATION OF NONCODING TRANSCRIPTION
Rampant transcription initiation occurring at the tens of thousands of cis-regulatory regions in the genome can be detrimental to the normal functioning of the cell. Several transcription termination mechanisms act to keep the initiation of non-productive transcription in check. The Restrictor …
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Regulatory RNAs at the Heart of Sugar Metabolism: New Mechanisms and Novel Discoveries
Bacteria are adept at using a variety of posttranscriptional strategies to regulate gene expression. Specifically, various RNA-mediated genetic control elements have been discovered in the past decade through a combination of genetics, bioinformatics, and transcriptomic approaches. Together, these …
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Investigation of the Substrate Recognition Characteristics and Kinetics of Mammalian Mitochondrial DNA Topoisomerase I
… (pZT-Hori and pZT-Lori, respectively), a major transcription termination region (pZT-Term) and a portion of cytochrome b gene (pZT-Cytb) were prepared. Two other recombinant plasmids, containing non-mitochondrial DNA inserts (pZT-800 and pZT-400) served as control substrates. Southern …
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Prion biology in the context of bacteria
… Moreover, we provide evidence that a bacterial transcription termination factor exhibits prion-like behavior in E. coli and yeast. Our work thus suggests that bacterial prions exist and may function as previously unrecognized reservoirs of phenotypic diversity among the most abundant organisms …
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PolyA signals located near 5’ of genes are silenced by a general mechanism that prevents premature 3’ end processing
… silent when they are positioned close to transcription start sites in either Drosophila or human cells. This suggests that the transcriptional stage when the polyA signal emerges from the polymerase II (Pol II) transcription complex could determine whether a putative polyA signal is …
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Structure-Function Relationships of Elements of the Bacteriophage f1 Genome
… were investigated by: 1) relocation of an intact transcription unit on the phage genome; 2) construction of a hybrid E. coli-f1 transcription unit which could be studied in a phage or plasmid context; 3) identification and characterization of a rho-dependent transcription termination signal in f1 …
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GATA1 Occupancy Site Regulates Erythroid Cell Fitness through Pleiotropic Functions
… grammar, complex chromatin environments, and the transcriptional regulatory networks that link genotype to phenotype. In this study, we combined base editor-mediated perturbations of regulatory elements, CRISPR-mediated gene disruptions, epigenetic profiling, and chromatin organization data with …
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Understanding the physiological role of the small RNA RydC
… ArrS and CpxQ, also directly regulate cfa post-transcriptionally. RydC and ArrS act through masking an RNase E cleavage site in the cfa mRNA 5' untranslated region (UTR), and both sRNAs post-transcriptionally activate cfa. In contrast, CpxQ binds to a different site in the cfa mRNA 5' UTR and …
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Multiscale dissection of bacterial proteome optimization
… in pathway operons. Genome-wide mapping of transcription termination sites also led to the discovery of a phylogenetically widespread mode of bacterial gene expression, 'runaway transcription', whereby RNA polymerases are functionally uncoupled from pioneering ribosomes on mRNAs. To …
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Design, Construction, and Screening of an shRNA Library Targeting Human Circular RNAs
… expression are prone to artifacts as bypassing transcription termination results in RNA concatamers. To better characterize the function of circRNAs, we developed a novel pooled library of ~ 15,000 shRNAs targeting ~ 5,000 circRNAs. We performed a loss-of-function screen with the circRNA shRNA …
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Changes in the Rpb3 Interactome Caused by the Deletion of RPB9 in Saccharomyces cerevisiae
… II (Pol II) is the primary actor in the transcription of mRNA from genes. Pol II is a complex composed of twelve protein subunits. This study focused on the changes in the interactome of Rbp3 in S. cerevisiae when the Pol II subunit Rpb9 is removed. Rpb3 is one of the core subunits of Pol …
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