Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 1771 for “"transcription factor"”.
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Recording Transcription Factor Binding During Development
… a novel method for mapping the binding sites of transcription factors using transposons to insert into the genome near to where the factors are bound. The method, named "Calling Cards", offers an alternative to chromatin immunoprecipitation (ChIP) methods and furthermore has the ability to record …
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Transcription Factor Engineering: Tools and Applications
Transcription factors play a vital role in the biology of every organism. By controlling gene expression they regulate growth, development, metabolism, reproduction, signaling, and response to the environment. They have also provided the basis for many useful tools in molecular biology. The …
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Synthetic Transcription Factor Allostery Mapping and Analysis
… a deeper understanding towards position linked factors integral in LacI allostery. Ultimately, this research seeks to establish a foundation for improved engineering strategies for synthetic transcription factors and to enhance the development of associated machine learning models by further …
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Identification of transcription factor genes in plants
… this control is to regulate the initiation of transcription. This step requires the assembly of several components, i.e., a basal/general machinery common to all expressed genes, and a specific/regulatory machinery, which differs among genes and is the responsible for proper gene expression in …
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Dissecting genetic determinants of transcription factor activity
… of the central goals of biology for a long time. Transcription factors (TFs) play a mediating role connecting genotype with gene expression, which provides high-dimensional information about end phenotype. In particular, gene expression levels depend on their cis-regulatory sequence bound by TFs …
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Inferring the properties of transcription factor regulation
The regulatory targets of transcription factors are costly to directly detect using existing technologies. Many computational models have thus been developed to infer the genes targeted by TFs using gene expression profiles, position weight matrices modeling TF protein binding, histone …
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Structure and Function of Bordetella Transcription Factor BpsR
… is under repressive regulation by a MarR-family transcription factor Bordetella polysaccharide regulator, BpsR. BpsR binding inhibits expression of the bps locus, and leads to a decrease in polysaccharide available for biofilm formation. The goal of this thesis was to evaluate BpsR regulation at …
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On the Diversity of Eukaryotic Transcription Factor Specificities
Transcription factor (TF) DNA-binding specificities (motifs) are often conserved between species, and many TFs have conserved functions over long evolutionary distances. As a consequence, changes in gene expression are largely attributed to evolution of cis-regulatory DNA sequences rather than …
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Modulation of Transcription Factor Activity by Mono-Ubiquitin
… and non-proteolytic roles in the regulation of transcription. We recently reported that the ATPases of the 26S proteasome can destabilize activator-DNA complexes in a non-proteolytic manner that requires direct interactions between the Rpt4 and 6 subunits with the activation domain of the …
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Preinitiation Complex Assembly and General Transcription Factor IIA
<p>The process of transcription is investigated biochemically from several perspectives. First, a novel transcriptional activator, GaI4-IE, is shown to activate transcription in vitro from GaI4-DNA binding sites located upstream of two core promoters. The mechanism of activation is investigated by …
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Transcription factor regulation of T helper subset function
… subsets of T helper cells is governed by transcription factors. Previous work demonstrated a requirement for the transcription factor PU.1 in the development of IL-9-secreting Th9 cells. Work in this dissertation demonstrates that the Th9 subset is not stable in vitro, and that PU.1 …
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Cell fate reprogramming using transcription factor feedback overexpression
… capture system dynamics and on inefficient transcription factor overexpression. We study a gene regulatory network that determines the cell fate in the hematopoietic lineage and demonstrate that a deterministic model cannot capture the experimentally observed system dynamics. We also propose …
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A Molecular Characterization of Human General Transcription Factor IID
<p>The general transcription initiation factor IID plays a central role in transcriptional control as a direct target for a diverse array of gene-specific regulatory factors and as the only template-bound class II initiation factor. The work described in this thesis concerns itself with the …
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Nuclear proteomics and transcription factor profiling in Chlamydomonas reinhardtii
The transcriptional regulation of the cellular mechanisms involves many different components and different levels of control which together contribute to fine tune the response of cells to different environmental stimuli. In some responses, diverse signaling pathways can be controlled …
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Differences of DNA-Transcription Factor Interactions Following Chromium Exposure
… observed to change the binding affinity of the transcription factor for the êB DNA sequences at each modified guanine site. The protocol used a p50 binding domain protein (residues 23 to 366 from the p50 protein) and a p50 mutant protein with a single amino acid mutation of the wild type …
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Differences of DNA-Transcription Factor Interactions Following Chromium Exposure
… observed to change the binding affinity of the transcription factor for the êB DNA sequences at each modified guanine site. The protocol used a p50 binding domain protein (residues 23 to 366 from the p50 protein) and a p50 mutant protein with a single amino acid mutation of the wild type …
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Functional analysis of transcription factor mScrt2 in cortical neurogenesis
Der Neokortex ist eine für das Säugerhirn spezifische Struktur, die schichtartig das Vorderhirn umschließt. Er zeichnet sich durch eine enorme Zellvielfalt aus, die aus der räumlich-zeitlich koordinierten Generierung mehrerer neuronaler und glialer Zelltypen hervorgeht. Die Mehrheit aller …
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Transcription factor Pax6 protects mouse cortical cell fate specification
… is regulated by differential expression of transcription factors (TFs). Pax6 is one of such TFs. Importantly, it is expressed strongly in the PC dorsal Emx1 lineage but much less so or not at all in interneuron ventral lineages. Here I investigated the postnatal characteristics of a Cre-loxP …
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