Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"transcription activity"”.
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The Role of DNA Binding in Transcription Activation by Estrogen Receptor
… exhibited 2-3 fold increased hormone-dependent transcription. Using a minimal promoter containing two copies of the estrogen response element (ERE) linked to a TATA box, it exhibited a 10 fold increase in hormone-independent constitutive transcription activity compared to that of the wild-type …
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An investigation into the role of Bcl-3 in toll-like receptor signalling
… regulates NF-κB dependent inflammatory gene transcription following TLR activation. Firstly, we investigated the nature of the interaction between Bcl-3 and p50 and using peptide array technology. Key amino acids required for the formation of the p50:Bcl-3 immunosuppressor complex were …
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Transcriptional Regulation Mediated by Human Papillomavirus E2 Protein
… proteins in concert with host cellular transcription factors. Using reconstituted cell-free transcription systems, cellular enhancer-binding factors and general cofactors, such as TAFIIs, TFIIA, Mediator, and PC4, were found not to be required for E2-mediated repression. HPV E2 is able …
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Identification and Characterization of Transcriptional Regulatory Elements for Liver-Specific Expression of Rabbit CYP2C Genes
… element is a binding motif of a liver-enriched transcriptional factor hepatocyte nuclear factor 4 (HNF-4), since it was recognized by a nuclear protein with the same tissue distribution, DNA binding specificity and immunochemical reactivity as those of HNF-4. The CYP2C2 promoter-luciferase …
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Estrogenic and androgenic endocrine disrupting activity and risk assessment of phytoestrogens in Kenyan soya-based weaning flours
… respectively) were used to characterise the transcription activity of ten PEs (apigenin, daidzein, daidzin, genistein, genistin, enterodiol, equol, formononetin, glycitein and matairesinol) and in the Kenyan based gruel/flour extracts. A modified Hygiene Based Margin of Safety (HBMOS) method …
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STUDIES ON THE EFFECTS OF SOME FLAVONOIDS ON DNA TRANSCRIPTION AND CLONING OF LIPOAMIDE DEHYDROGENASE GENE
An in-vitro run-off transcription system was utilised to study the effects of flavonoids on DNA transcriptional processes. The template DNA consists of a gene coding for the chicken lysozyme protein inserted in a vector plasmid. Following conventional molecular biology methodology, large quantities …
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Curbing environmental pollution of water hyacinth by using it in drug discovery
… (ARE) promoter and tumour suppressor (p53 transcription activity) mechanisms, during extract treatment; and (4) Evaluate the effect of extracts and bioactive compounds on male fertility parameters and the effect of the most potent extract on spermatogonia stem cell viability.<br/><br/>We …
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Identification of genes involved in macrophage activation and effector functions against intracellular pathogens
… L. monocytogenes due to impaired listericidal activity. Comparison of the gene expression profiles of WT and C/EBPβ-/- macrophages infected with L. monocytogenes was postulated to increase the probability of identifying these effector genes, which would be differentially expressed between the …
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Structural and Mechanistic Roles of Novel Chemical Ligands on Quorum Sensing Transcription Regulator SdiA in Enterohemorrhagic Escherichia coli
… Gram-negative bacteria use the LuxR family of transcription factors to regulate gene expression to coordinate population behavior by sensing endogenously produced chemical signaling molecules, acyl-homoserine lactones (AHLs) [1]. However, some bacteria such as Escherichia coli (E. coli) do not …
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Characterization of Bacterial Transcriptional Regulatory Networks through Genome-Wide in vitro Run-Off Transcription RNA-Seq (ROSE)
The current methods for characterizing transcriptional regulatory networks are limited to in vivo conditions, providing a snapshot of multiple regulatory interactions simultaneously. To complement these approaches, a new method called ROSE (Run-Off transcription/RNA-sequencing) has been developed …
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Essential features of a PrfA-dependent : promoter of Listeria monocytogenes
… by PrfA that belongs to the Crp-Fnr family of transcriptional activators. A PrfA-mediated transcription initiating at a virulence gene promoter, inlC promoter (PinlC) that regulates the expression of the small, secreted internalin C, was in-depth characterized by an in vitro transcription …
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Cellular decision making: from phage lambda to stem cells
… are typically maintained and stabilized by the activity of specific genes, but cells can also be induced to switch to alternative states given the appropriate stimulus. Underlying decision making processes that result in different cell states are temporally regulated gene expression cascades. …
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REGULATION OF CANCER METASTASIS BY PROTEIN KINASE D1: A GLOBAL REGULATORY CASCADE
… overexpression leads to inhibition of β-catenin transcription activity in colon cancer cells. Further evaluation in in vivo mouse model showed that PKD1 is responsible for inhibition of colon cancer tumor growth in xenograft mouse model. This paved way for us to look for the effect of PKD1 on …
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Genistein suppresses colon cancer metastasis through epigenetic regulations
… relate to metastasis. Importantly, the lower transcription of Wnt5a in SW620 than SW480 corresponded to multiple histone modifications, including lower levels of acetylated histone H3 and H4 (H3Ac and H4Ac), dimethyl H3 lysine 4 (H3K4me2), and higher levels of trimethyl H3 lysine 27 (H3K27me3) …
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Impatto di rifaximina sul microbiota intestinale: selezione di bifidobatteri antibiotico resistenti
… absorption while retaining good antibacterial activity. Both experimental and clinical pharmacology clearly show that this compound is a non systemic antibiotic with a broad spectrum of antibacterial action, covering Gram-positive and Gram-negative organisms, both aerobes and anaerobes. Being …