Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"transcription activator-like effector (TALE)"”.
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Targeted Epigenetic Editing to Increase Adult Pancreatic Β-Cell Proliferation
… active enhancers. Further, I developed transcription activator-like effector (TALE) fusion proteins conjugated to DNA methyltransferases (DNMTs) and demonstrated that targeting TALE-DNMTs to the promoter of the CDKN2A locus, encoding the cell cycle inhibitor p16, increases proliferation …
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A versatile protein platform for sequence specific nucleic acid detection
… nucleic acid imaging and detection based on Transcription Activator-Like Effector (TALE) proteins with versatile functional domains, characterization of the platform, and its application in live cells, fixed cellular specimens, and cell-free context. In fixed and live cell specimens, I used …
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Cellular reprogramming in eukaryotes
… aim is the same – to be able to program cells like we do to robots today. Two general approaches will be discussed in this dissertation. In the first half, we will look into reprogramming via the introduction of external network components such as an artificial transcription factor (gene …
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Engineering of transcription activator-like effector nucleases (TALENs) for targeted genome editing
… This thesis describes my efforts on engineering transcription activator-like effector (TALE) nucleases (TALENs) as an efficient tool for targeted genome editing. Targeted genome engineering relies on the introduction of a site-specific double-strand break (DSB) in a pre-determined genomic locus …
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Mitochondrial genome engineering in the murine germline using designer nuclease technology
… either a dimeric zinc finger protein (ZFP) or a transcription activator-like effector (TALE) domain, is conjugated to a dimeric nuclease domain, producing zinc finger nucleases (ZFN) or TALE-nucleases (TALEN), respectively. By targeting these pairs of engineered nucleases adjacent to a site of …
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Study and engineering of nucleic acid-binding repeat proteins
From DNA replication and repair to transcription and translation, all aspects of molecular biology are governed by interactions between proteins and nucleic acids. Some of the nucleic acid-binding proteins have evolved repetitive structures and a modular sequence recognition mode. In particular, …