Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"transcription activator"”.
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Structure and Function Analysis of a Plant Transcription Activator
… in leaf. TGA1a can function as an as-1-specific transcription activator in a HeLa cell in vitro system as well as a wheat germ in vitro system. In both systems, TGA1a stimulates transcription by increasing the number of preinitiation complexes. When microinjected into cotyledon cells of …
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Single molecule investigation of transcription activator-like effector search dynamics
… and accessibility of gene engineering. Transcription activator-like effectors (TALEs) are one such class that form a powerful gene-editing platform when fused to nuclease domains, thereby yielding TALEN systems. Despite pervasive use of TALENs for editing crops, small animals, eukaryotic …
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Interaction of Bacteriophage Mu Middle Transcription Activator Protein Mor with Promoter DNA
… of bacteriophage Mu is regulated by a</p> <p>transcriptional cascade in three phases: early, middle and late. Transcription from the middle promoter Pm requires the 129-amino acid transcriptional activator Mor, a product of early transcription, and the Escherichia coli RNA polymerase. The Pm …
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Engineering of transcription activator-like effector nucleases (TALENs) for targeted genome editing
… This thesis describes my efforts on engineering transcription activator-like effector (TALE) nucleases (TALENs) as an efficient tool for targeted genome editing. Targeted genome engineering relies on the introduction of a site-specific double-strand break (DSB) in a pre-determined genomic locus …
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Innate Immune Transcription Activator Interferon Regulatory Factor-3 (IRF3) Contributes to Maladaptive Remodeling Post-myocardial Infarction
… and overall survival. While the well-known NF-κB transcriptional factor that is downstream to TLR4 signaling has been linked to remodeling, there has been no evidence thus far describing a role of the parallel interferon regulatory factor-3 (IRF3) signaling cascade in any facet of this process. …
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Structural and Functional Studies of the Unconventional Proteobacterial Transcription Activator Crl in Complex with the Transcription Machinery
… with the core RNA polymerase to initiate the transcription of genes needed to survive different environmental changes. Crl is a small protein that activates the transcription of σ<sup>S</sup>-dependent genes. In contrast to most transcription activators, Crl does not bind DNA to help recruit …
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Využitie transgénnych rastlín pri štúdiu funkcie rastlinných génov
… meganucleases, zinc finger nucleases, TALEN (transcription activator-like effector nucleases) and CRISPR (clustered regularly interspaced short paindromic repeats). In contrast to inactivation approaches, there are approaches such as activating mutagenesis and ectopic expression, through which …
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Serine/threonine phosphorylation in mycobacterium tuberculosis : identification of protein kinase B (PknB) substrates
… as the negative regulator of DevR transcription activator activity based on LC-MS/MS analysis. Finally, we have identified a previously unknown phosphoprotein glyceraldehyde 3-phosphate dehydrogenase encoded by the ORF Rv1436, which demonstrates autophosphorylation activity and …
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Modeling 3D Retinogenesis in Mouse Embryonic Stem Cells Following CRISPR-mediated Crx Knockdown
… like Zinc Finger Nucleases (ZFNs) or Transcription Activator-like Effector Nucleases (TALENs).</p> <p>In order to overcome current limitations on disease-specific model systems, we developed a protocol for differentiating mouse embryonic stem cells into 3D retinas <em>in vitro</em>. In …
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Regulation of the GCV3 gene in Saccharomyces cerevisiae
… used by the nitrogen regulation system. Gcr1p, a transcription activator for glycolytic genes, is involved in regulating GCV3 in the presence of glucose. Evidences are also presented for an as yet unidentified regulator that represses expression in SD. Additional results presented in this thesis …
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Characterization of NHLRC2 gene-edited mice: a model for bovine developmental duplications
… in the NHL repeat-containing 2 (NHLRC2) gene. Transcription activator-like effector nucleases (TALENs) targeting exon 5 of NHLRC2 were used for gene-editing of the orthologous locus in mice to further investigate the role of NHLRC2 in development. Three mouse lines were generated with mutations …
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DNA sequences required for efficient transcription of the estrogen regulated Xenopus laevis vitellogenin promoter
… regulated by estrogen. Estrogen activates gene transcription by binding as estradiol-estrogen receptor complex to estrogen response elements (EREs) in the 5$\sp\prime$-flanking region of target genes. In order to identify the DNA sequences essential for estrogen-regulated and liver-specific …
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Targeted Epigenetic Editing to Increase Adult Pancreatic Β-Cell Proliferation
… active enhancers. Further, I developed transcription activator-like effector (TALE) fusion proteins conjugated to DNA methyltransferases (DNMTs) and demonstrated that targeting TALE-DNMTs to the promoter of the CDKN2A locus, encoding the cell cycle inhibitor p16, increases proliferation …
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Mechanism Of Transcription Regulation And Stability Of The Quorum Sensing Protein Trar
TraR is a transcription activator of the quorum sensing system in the plant pathogen Agrobacterium tumefaciens. This protein is composed of two domains: amino-terminal domain (NTD) which is responsible for binding the autoinducer OOHL and for dimerization and the carboxyl-terminal domain (CTD) …
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LncRNA discovery in the Listeria monocytogenes infection model
… lncRNAs play important functional roles in the transcriptional regulation of a wide array of cellular processes. In the last year alone, a handful of studies have identified lncRNAs linc-Cox2, Lethe, PACER and THRIL as central players in host cell innate immune response against microbial …
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Exploiting rice diversity to uncover durable and broad-spectrum resistance
… screened with an Xoo strain containing Tal7b, a transcription activator-like (TAL) virulence effector that is common to many Xoo strains. We mapped disease resistance QTL unique to this specific virulence factor and hypothesize that the mechanism of resistance conferred by one QTL is through a …
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A versatile protein platform for sequence specific nucleic acid detection
… nucleic acid imaging and detection based on Transcription Activator-Like Effector (TALE) proteins with versatile functional domains, characterization of the platform, and its application in live cells, fixed cellular specimens, and cell-free context. In fixed and live cell specimens, I used …
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Genetic and Biochemical Characterization of the Mechanisms Concerning the TraR/AAI/TraM Mediated Quorum Sensing That Regulates Ti Plasmid Conjugation
… system consisting of four elements; the transcription activator TraR, the intercellular signal N-(3-oxo-octanoyl)-L-homoserine lactone, the cis-acting recognition site tra box located in the promoter region of the target gene and the antiactivator TraM that negatively modulates TraR …
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Post-transcriptional regulation of vibrio cholerae virulence activator toxt
… on. ToxT protein is the primary virulence gene transcription activator. Once ToxT is produced, it amplifies its own expression through an auto-regulatory loop and directly binds and activates expression of various virulence factors including the toxin-coregulated pilus (TCP) and cholera toxin …
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Cellular reprogramming in eukaryotes
… network components such as an artificial transcription factor (gene switch) or a pathway. In one example, we report the development of a gene switch that is able to regulate the endogenous VEGF-A expression in mammalian cell. The gene switch is specifically and reversibly controlled by …
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