Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"transcript stability"”.
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Identification of substrate-selective histone deacetylases and their inhibitors that mediate HER2 transcript stability
… can induce the rapid decay of oncogenic transcripts such as the HER2 mRNA, though the mechanism underlying this process remains undefined. Earlier observations in our lab led to speculation that protein(s) binding the 3’-untranslated region (UTR) of HER2 mRNA become acetylated following …
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Intracellular Traits of Chimeric tRNAlys3-ribozymes and their Inhibition of HIV
… primer recruited by HIV-1 for initiating reverse transcription. When ribozymes targeted upstream of the primer binding site of the HIV-1 genome are tethered to the 3' end of the tRNALYs3, it is expected that the tRNA will carry the ribozymes into the virions. We made constructs that express …
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Regulation and Function of the Dscam1 Extended 3' UTR mRNA
… tissues are highly enriched for usage of transcripts with extended alternative 3' untranslated regions, which are products of APA. Specific function of these longer 3' UTR transcripts are largely unknown; however, because the 3' UTR can regulate transcript stability, translational …
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Therapeutic Targeting Of Bmp2 In Nf1-Deficient Malignant Peripheral Nerve Sheath Tumors (mpnsts)
… inhibition, and use the regulation of BMP2 transcriptional control to define mechanism-based rational targets of BMP2 signaling, to treat NF1-related tumors. </p> <p>Biological aggressiveness of MPNSTs is characterized by its highly motile and invasive nature. I presented a unique approach …
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Expression Of Hepatitis C Viral Non-structural 3 Antigen In Transgenic Chloroplasts
… in the light and 3' untranslated region for transcript stability and trnI & trnA homologous flanking sequences for site specific integration into the chloroplast genome. Chloroplast integration of the NS3 gene was first confirmed by PCR. Southern blot analysis further confirmed site-specific …
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Investigating the Role of Muscleblind-Like 1 in the Suppression of Breast Cancer Progression
… determinants of breast cancer progression. Post-transcriptional control of gene expression plays a central role in modulating transcriptional output. The interactions between messenger RNA cis-regulatory elements and trans-factors control coordinated gene expression states. Posttranscriptional …
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Rox1 function in dosage compensation: structural / functional analysis of a non-coding rna
… occurs by a global two-fold increase of transcription from the single male X chromosome. This essential process compensates for X chromosome monosomy. The male-specific lethal (MSL) complex, containing five proteins, localizes to the male X chromosome and alters chromatin to modify gene …
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RNA Deregulation in Metastatic Breast Cancer
… it is becoming increasingly apparent that post-transcriptional modification of gene expression at the RNA level contributes substantially towards gain of metastatic potential. In eukaryotic cells RNA is subject to multiple layers of regulation that together generate extensive phenotypic and …
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Role of the Hu family of RNA-binding proteins in regulating acetylcholinesterase expression in excitatory cells
Post-transcriptional mechanisms regulating acetylcholinesterase (AChE) expression in muscle and neurons are relatively ill-defined, even though several studies have implicated mRNA stability as a means of controlling expression. We have, therefore, undertaken studies examining the role of …
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The Influence of ampC Expression and Penicillin Binding Protein Binding on the Variation of ß-lactam Induction Potential in Three Genera of Enterobacteriaceae
… The 5'UTR of the S. marcescens ampC transcript forms a stem-loop structure and is involved in regulating the stability of the transcript while nothing is known about the role of the 5'UTR of the ampC transcripts of C. freundii and E. cloacae plays in transcript stability. Preliminary …
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Multigene Metabolic Engineering Via The Chloroplast Genome
… integration into the chloroplast genome and transcription of the phaA gene coding for β-ketothiolase was confirmed by Southern and northern blots. Coomassie-stained gel and western blots confirmed hyperexpression of β-ketothiolase in leaves and anthers, with high enzyme activity. The …
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Investigations of cellular stress-responsive proteins and PGF gene expression in human trophoblast
… stress-mediate angiogenic responses include post-transcriptional regulation of mRNA stability, and regulation of PML sequestering protein. We investigate whether these mechanisms occur in hypoxic stressed trophoblast and preeclamptic placenta. Methods: To determine transcript stability, PGF mRNA …
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Sindbis virus usurps the cellular HUR protein to stabilize its transcripts and promote infections of mammalian and mosquito cells
… of cellular mRNAs as the decay of Sindbis virus transcripts is predominantly deadenylation-independent. As cellular mRNAs are often regulated by elements present in their 3’ untranslated regions (UTR), we hypothesized that these viral 3’UTR elements were functioning similarly to cellular mRNA …
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New Insights into the Regulatory Network Controlling Neuroblast Migration in C. elegans
… processing, including regulating translation, transcript stability and alternative splicing. Therefore we were interested in identifying targets of ETR-1 in C. elegans. We isolated muscle cells using fluorescence-activated-cell-sorting (FACS) and performed RNA-sequencing on isolated RNA. Using …