Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"trans-splicing"”.
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Genomics and Transcriptomics Analysis of the Asian Malaria Mosquito Anopheles stephensi
… Here we conducted a series of genomic and transcriptomic studies to improve the understanding of the biology of Anopheles stephensi and mosquito in general. First we reported the genome sequence and annotation of the Indian strain of the type form of An. stephensi. The 221 Mb genome …
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Regulation of Gene Expression in Trypanosoma brucei
… Atypical for a eukaryote, in T. brucei genes transcribed by RNA polymerase II (RNA pol II) are arranged in polycistronic transcription units (PTUs). mRNAs are separated post-transcriptionally by coupled splicing and polyadenylation reactions. During the splicing reaction a 39 nt …
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The Effects of Polysomal mRNA Association and Cap Methylation on Gene Expression in <i>Trypanosoma brucei</i>
… and therefore changes in protein expression. Transcriptional regulation is unusual in T. brucei because the arrangement of genes is polycistronic; however, genes which are transcribed together are subsequently cleaved into separate mRNAs by trans-splicing and are individually regulated. During …
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Development of a Lentivirus-mediated Gene Therapy Targeting HIV-1 RNA to Eliminate Infected Cells
… the currently unexploited HIV-1 alternative RNA splicing process to functionalize a defective cell suicide enzyme (*HSVtk*<sub>ΔAUG1</sub>) through targeted RNA *trans*-splicing with the HIV-1 D4 splice site. In-frame *HSVtk* translation is initiated from the start codon of the HIV-1 *tat1* donor …
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The mRNA Cap and Discontinuous Transcription in Trypansoma brucei
… all, mRNA. Indirect evidence suggests that trans-splicing, rather than RNA priming of transcription, is the mechanism by which the mini-exon is joined to RNA containing protein-coding sequences. In vivo <sup>32</sup>P-labeled RNA was purified by hybrid selection with mini-exon genomic DNA, …
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A Selection for Intein Splicing inPhage Assisted Continuous Evolution
… remains a longstanding goal in chemical biology. Trans-splicing inteins fulfill a crucial niche in this area by enabling post translational splicing of separate polypeptides, introduction of post translational modifications, and other chemistry on the amide backbone. This has motivated engineering …
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Engineering RNA logic with synthetic splicing ribozymes
… In this thesis, I show the utility of splicing ribozymes for the biological engineer. Ribozymes allow the engineer to manipulate existing biological systems and to program self-modifying RNA systems. In addition, splicing ribozymes are easy to engineer, malleable, modular, and scalable. …
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New Biological Pathways
… viral capsid proteins to perform non-destructive transcriptomic measurements. We demonstrate that this approach allows for live-cell transcriptomics, and we longitudinally measure the transcriptional responses of the same living human cells after stimulation with TNFa. For information storage, I …
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Massively parallel analysis of the functional effects of mutations
… effects on protein expression due to changes in splicing patterns. In Chapter 4, I discuss initial work developing a new method for linking genotypes found in the same cell by using trans-splicing ribozymes, a technology that could enable massively parallel all-by-all screening. In Chapter 5 I …
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Structural and Biophysical Characterisation of Denatured States and Reversible Unfolding of Sensory Rhodopsin II
… pSRII retains the equivalent of six out of seven transmembrane helices, while the retinal binding pocket is disrupted, with transmembrane residues becoming more solvent-exposed. Folding of pSRII from an SDS-denatured state harbouring a covalently-bound retinal chromophore shows deviations from an …
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The Role and Fate of Branch Site-U2 snRNA Interaction During Pre-mRNA Splicing
… of the conformational dynamics underlying transitions during premRNA splicing, particularly during the catalytic phase, remain unclear despite our deep knowledge of spliceosome composition. The complex assembly phase that precedes catalysis, and the involvement of several motifs in multiple …
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THE DIVALENT CATION TRANSPORTER NRAMP IN PARASITE PERKINSUS MARINUS: GENOMIC, MOLECULAR, STRUCTURAL, FUNCTIONAL AND EVOLUTIONARY ASPECTS
… Macrophage Protein (Nramp), an iron transporter, was considered to be a potential virulence factor in intracellular pathogens. One Nramp homolog (PmNramp1) was reported in P. marinus previously. Two other PmNramp isotypes (PmNramp2 and PmNramp3) were identified through genome mining …
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ADVANCED AAV-MEDIATED LIVER-DIRECTED GENE THERAPIES FOR HAEMOPHILIA A AND MUCOPOLYSACCHARIDOSIS TYPE VI
… significant promise for providing long-term transgene expression following a single systhemic administration. However, both the limited cargo capacity (~4.7 kb) and the non-integrative nature of AAV vectors prevent their application to conditions caused by either mutations in large genes …
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Split Inteins: From Mechanistic Studies to Novel Protein Engineering Technologies
… protein domains that carry out a post-translational process known as protein splicing. This process is characterized by excision of the intein (intervening protein) domain from within a larger polypeptide sequence with concomitant ligation of the flanking extein ( external protein) …
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Untersuchungen zum mRNA Trans-Spleißen bei den humanparasitischen Cestoden Echinococcus multilocularis und Echinococcus granulosus
SL-Trans-Spleißen ist ein Mechanismus zur Transkriptprozessierung, welcher bisher bei kinetoplastiden Protozoen, Trematoden und Nematoden beschrieben wurde. Im Rahmen der vorliegenden Arbeit wurde erstmals das Gen für einen Spliced Leader (SL) aus den Cestoden E. multilocularis und E. granulosus …