Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 38 for “"trans-Golgi Network"”.
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Role of trans-Golgi network vesicles in mitochondrial fusion
… fusion and fission are balanced, creating a network of mitochondria in the cell of intermediate length and moderate interconnectivity. However, when cells are stressed, for example by nutrient surplus or deficit, the population of mitochondria fragments or elongates, respectively. While …
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PI(4)P-Dependent Recruitment of Clathrin Adaptors to the Trans-Golgi Network
The Trans Golgi Network (TGN) is the cell's central sorting station, and the complex trafficking patterns are organized by many types of trafficking adaptors. These include the heterotetrameric adaptor protein complexes (APs) and the monomeric Golgi-localized, gamma-ear containing, Arf-binding …
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Identifikation des Transportproteins PACS-1 als Nephrocystin-interagierendes Protein
… In der vorliegenden Arbeit konnten wir das Transportprotein PACS-1 als Nephrocystin-interagierendes neues Protein identifizieren. Nephrocystin wird kodiert durch das NPHP1-Gen, welches bei Familiärer Juveniler Nephronophthise (FJN, NPH1) mutiert ist. Nephrocystin ist ein Multiadapterprotein …
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Yeast Dynamin Functions with Clathrin at the Golgi
… endocytosis, but also traffic from the Golgi to endosomes. In this study, the interdependency between Vps1 and clathrin was investigated. Subcellular localization of clathrin was at the Golgi, early and late endosomes, implicating clathrin in multiple trafficking pathways within the …
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The Role of Vps54 in Drosophila melanogaster Neuronal Development and Age Progressive Neurodegeneration
<p>Vps54 is a subunit of the Golgi-associated retrograde protein (GARP) complex, which is involved in tethering endosome-derived vesicles to the trans-Golgi network (TGN). The “wobbler” mouse is the phenotypic result of a destabilizing point mutation in Vps54. This mutation causes neurodegeneration …
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Double-stranded RNA uptake in Caenorhabditis elegans
… In the nematode Caenorhabditis elegans, a transmembrane protein named systemic RNAi deficient-2 (sid-2) is required for dsRNA uptake from the environment through the intestine. Furthermore, the tyrosine-protein kinase sid-3 promotes import dsRNA. However, the molecular mechanism underlying …
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A novel principle for immunomodulator delivery with hematopoietic cells as vehicles Focus on soluble TNF-receptor gene expression, targeting to secretory lysosomes and regulated secretion
… hematopoietic cells as vehicles for targeting transgenic immunomodulators to the inflammatory sites. The work presented was focused on the anti-inflammatory soluble TNF receptor. A transmembrane soluble TNF receptor (sTNFR1) construct was expressed in hematopoietic cell lines. ER export was …
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Biological studies of organellar (Na⁺,K⁺)/H⁺ exchanger NHE7
… of NHE7 from the recycling endosomes to the trans-Golgi network (TGN). In agreement with this finding, I found that NHE7 can also be targeted to the plasma membrane and then internalized. Caveolins, structural proteins for caveolae, were identified as NHE7-binding proteins and it was …
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Exploring the Impact of Endothelial Bone Morphogenetic Protein Receptor 2 Loss on Phosphoinositide and Cytoskeletal Assembly Dynamics and the Pathobiology of Pulmonary Arterial Hypertension
… of intracellular trafficking between the trans-Golgi network (TGN) and plasma membrane (PM) is vital for protein secretion and growth factor signaling, which involves recycling and PM localization of cell surface receptors. These processes depend on the cytoskeleton and the presence of …
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Somatostatin Receptor 2 Regulation By C-Terminal Motifs and Pdz Domain Proteins
… endosomes to late endosomes and then on to the trans-golgi network (TGN). Trafficking from late endosomes to the TGN is dependent upon an intact C-terminal tail and the retromer complex of endosomal coat proteins. Additionally, removing either 3 or 10 C-terminal amino acids from sst2 alters the …
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Microarray Analysis Identifies Novel Cholesterol Regulated Genes, Including Pcsk9 Which Regulates LDL Receptor Function and LDL Cholesterol Levels
… important in these processes are regulated at a transcriptional level by the sterol regulatory element binding proteins (SREBPs) and liver X receptors (LXRs). This thesis describes the identification by microarray technology of novel genes in cholesterol metabolism and the characterization of two …
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The Functional Synergy Between Yeast Dynamin and Key Membrane Recycling Factors
… which cells recycle endocytic materials from the trans-Golgi network or endosome back to the cell membrane, as opposed to being targeted to the vacuole for degradation. Ypt31p and Ypt32p GTPases are known to regulate the function of F-box protein Rcy1p, which mediates recycling of the plasma …
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Presentation of Recombinant Proteins in Modified Vaccinia Virus Ankara Extracellular Enveloped Virions
… vaccinia virus IHD-J is more efficient in trans-Golgi-network-wrapping and in releasing enveloped virions into the extracellular medium, while the WR strain is less efficient than MVA. Two different strategies were followed to target the recombinant protein to the surface of extracellular …
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Sorting of Cargo Proteins Within the Regulated Secretory Pathway: the Peripheral Membrane Protein HID-1 as a Sorting and Vesicle Biogenesis Factor
<p>Large dense core vesicles (LDCVs) form at the trans-Golgi network (TGN) and mediate the regulated release of neuropeptides and peptide hormones. Despite their central role to physiology, the mechanisms controlling biogenesis and sorting to LDCVs is not well understood. Optimizing the retention …
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Packaging and Secretion of Airway Mucins
… machinery become associated with granules in the trans-Golgi network? Does the structure of Golgi bodies in airway secretory cells change with mucous metaplasia?</p>
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Regulatory domains of the Arf GEF Sec7 scaffold autoinhibited and active conformations
The Golgi apparatus is a highly modular organelle that mediates the secretory pathway in eukaryotic cells. The final compartment, the trans-Golgi Network (TGN), connects the Golgi to the endomembrane system and balances the flow of membranes and cargo throughout the cell. Virtually all sorting and …
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An investigation of the function of adaptor protein complex 4 (AP-4)
… are selectively sorted into membrane bound transport intermediates by vesicle adaptors, which include those of the highly conserved adaptor protein (AP) complex family. Each AP complex has a distinct subcellular localisation and functions in the sorting of a specific subset of transmembrane …
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The interrelationship between the endosomal sorting regulators RAB21, Retromer and WASH complex
… to different subcellular locations (such as the trans-Golgi network or recycled back to the plasma membrane) or the cargo is directed to be degraded in the lysosome. Issues in endocytosis have been implicated in the development of neurodegenerative diseases, and cardiovascular and inflammatory …
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Membrane trafficking of ATP-sensitive potassium channels
… numbers is due to an increase in the rate of translation of pre-existing mRNA and may be mediated by the activation of AMP-activated protein kinase. Despite the - 2.5 fold increase in channel numbers only a small, but non significant, difference in cell surface density was observed as …
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Functional Characterization of the HIV-1 NEF Acidic Cluster
… Nef's redirection of cell surface MHC-I to the trans-Golgi network by binding to the cytosolic adapter phosphofurin acidic cluster sorting-1 (PACS-1) (19, 147). Because Nef is potentially significant to the course of disease progression, correlations between the amino acid composition of the Nef …
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