Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"tradis"”.
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Barcoded Transposon Directed Insertion-site Sequencing (TraDIS); a tool to improve our understanding of the functional genomics of Streptococcus equi subsp. equi
… transposon directed insertion-site sequencing (TraDIS) technologies provides an opportunity to simultaneously determine the importance of every gene in S. equi under disease relevant conditions, significantly enhancing the capacity to identify new vaccine targets. In this project, a novel …
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Using high-density mutagenesis to identify the genetic requirements for the growth of Escherichia coli
… transposon-directed insertion site sequencing (TraDIS) to describe genetic requirements for fitness of E. coli K-12 MG1655 during growth in the presence of bile and under anaerobic conditions in the presence of nitrate, representative of bile exposure in the duodenum and conditions of …
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Towards Understanding the Genetic Basis of Virulence in Pseudomonas aeruginosa
… the high throughput sequencing based method, TraDIS (Transposon Directed Insertion-site Sequencing) to simultaneously screen pooled P. aeruginosa transposon mutants for genes that are essential for metabolism and biosynthesis of essential nutrients. Pooled mutants were cultured in the presence …
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High-throughput experimental and computational studies of bacterial evolution
The work in this thesis is concerned with the study of bacterial adaptation on short and long timescales. In the first section, consisting of three chapters, I describe a recently developed high-throughput technology for probing gene function, transposon-insertion sequencing, and its application to …