Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 31 for “"traction forces"”.
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Computing traction forces, intracellular prestress, and intracellular modulus distribution from fluorescence microscopy image stacks
… The dot deformation provides a measure of traction forces acting on the cell. From traction forces thus inferred, we use FEM to compute the stress distribution within a cell. We consider two approaches. The first is based on the assumption that the cell has homogeneous elastic properties. …
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Investigating Mechanotransduction and Mechanosensitivity in Mammalian Cells
… that cells are also influenced by the mechanical forces and mechanical properties of the local microenvironment. Extracellular forces causing cellular deformation can originate from many sources, such as fluid shear stresses arising from interstitial or blood flow, mechanical stretching during …
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Dendritic Cell Migration and Traction Force Generation in Engineered Microenvironments
… detectors to develop a novel method for probing traction forces during chemotaxis. We find DC migration is driven by short-lived traction stresses at the leading edge or filopodia. We illustrate that spatiotemporal pattern of traction stresses can be used to predict changes in the direction of DC …
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Cellular decision making at the nanoscale
The well-established dependence of cell traction forces on the compliance of supporting matrices has been attributed to levels of force exerted on components in focal contacts. Here, use of novel, force-limited nanoscale tension gauges revealed that both force and substrate deformations govern cell …
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A Statistical Approach to Modeling Wheel-Rail Contact Dynamics
… exist in practice to longitudinal and lateral tractions and to develop parametric models that can be used for predicting traction in conditions beyond those tested on the rig. The experiment-based models are intended to be an alternative to the classical traction-creepage models that have been …
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Experimental and Computational Analysis of Cell Mechanics during Spreading and Migration
… Cells use their ability to generate mechanical forces to probe the mechanical resistance of their environment and move forward. Biomechanical explanations for how mechanical factors in their environment affect these forces and how cells use their forces to migrate are still unclear. Here, using …
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Engineering Substrates for the Study of Cell Mechanical Interactions
… a novel approach to measure and map protrusive forces in cells and the application of fabrication techniques like wet etching and microcontact printing to answer fundamental biological problems. In order to isolate the effects of curvature from other factors, we have developed a technique to …
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Accurate Wheel-rail Dynamic Measurement using a Scaled Roller Rig
… the wear of the wheel and/or roller) on creep or traction forces. The wheel-rail contact forces, also referred to as traction forces, are critical for all aspects of rail dynamics. These forces are quite complex and they have been the subject of several decades of research, both in experiments and …
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CELL GEOMETRIC CONSTRAINTS REGULATE NUCLEAR AND CHROMATIN PLASTICITY VIA ACTOMYOSIN CONTRACTILITY
… which nuclear and chromatin dynamics respond to traction forces at cell periphery in unperturbed cells. In the second project, using nuclear envelope fluctuations and heterochromatin dynamics as readout, we characterized how cytoskeletal forces alter depending on the EMS provided by …
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Biomimetic cytoskeleton assemblies and living cells on micropillar force sensor arrays
… and heart muscle cells on their tops. Cellular traction forces are determined by measuring the bending of the pillar tops during cell movement. A model system for the actin cortex is produced by crosslinking actin filaments on the pillar heads, with the actin binding protein filamin. …
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Development of a novel cell traction force transducer based on cholesteryl ester liquid crystals. Characterisation, quantification and evaluation of a cholesteryl ester liquid crystal based single cell force transducer system.
… resolution or ability to detect localised cell traction forces (CTF) due to their non-linear viscous behaviour under shear rates. A newly developed cell traction force transducer system based on cholesteryl ester lyotropic liquid crystals (LCTFT) was developed to sense localised traction forces …
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PROBING CELLULAR MECHANO-SENSITIVITY USING BIOMEMBRANE-MIMICKING CELL SUBSTRATES OF ADJUSTABLE STIFFNESS
… morphology, cytoskeletal organization, cellular traction forces, and migration speed. Furthermore, we illustrate the dynamic assembly of bilayer-bound N-cadherin linkers underneath cellular adherens junctions. In addition, properties of individual and clustered N-cadherins are examined in the …
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THE NON-ERYTHROID MEMBRANE SKELETON AND ITS ROLE IN MECHANOTRANSDUCTION
… of the spectrin membrane skeleton in transducing forces to and from non-erythroid mammalian cells. This spectrin based membrane skeleton has been the focus of much study in the context of red blood cells, as it determines their mechanical properties due to the lack of an extended actin based …
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Homeostatic mechanics framework for modelling interactions of cells with the microenvironment
… substrates, concomitant with increased cellular traction forces. The variability in morphometrics (such as cell shape, area, cytoskeletal protein arrangements and traction forces) also reflects in other critical cell functionality. In particular, the fluctuating response of stem cells on …
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Harnessing the Regenerative Characteristics of Meniscus Superficial Zone Cells
… assays, mechanosensation analysis, and traction force microscopy. After, the matrix deposition potential of superficial cells was assessed on electrospun nanofibers. Here, we show at the transcriptional level that superficial cells are most similar to those of the meniscus body, while …
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The role of tissue viscoelasticity in axon guidance during Xenopus brain development
… to simulate axon velocity, retrograde flow, and traction forces using elastic and viscoelastic frameworks. I found that the viscoelastic motor clutch model better described differences in axon lengths from the in vitro explant experiments. Furthermore, I found that the viscoelastic motor clutch …
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INVESTIGATING THE ROLE OF LIPID DROPLETS AS INTRACELLULAR MECHANICAL STRESSORS IN NON-ALCOHOLIC FATTY LIVER DISEASE
… cytoskeletal network organization, reducing cell traction forces and preventing proper stiffness sensing. Mathematical modelling of lipid droplets as inclusions that have only mechanical interactions with other cellular components generated results consistent with our experiments, further …
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Development of a Model System for Culturing Neonatal Rat Ventricular Myocytes in vitro to Monitor Integrin Activation in Response to Altered Contractile Force
… the HCM disease state. The interplay between the forces generated by the contractile apparatus and sensed by integrins is central to understanding the pathogenesis of HCM. Integrins are likely the mechanotransducers in cardiomyocytes, converting mechanical stress into chemical signals that …
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Cell Mechanics: From Cytoskeletal Dynamics to Tissue-Scale Mechanical Phenomena
… theoretical description of the dependence of traction forces on cell size, actomyosin activity, matrix depth and stiffness, strength of focal adhesions and makes experimentally testable predictions. In part (c) we combine experiment and theory to reveal how intercellular adhesions modulate …
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Evaluation of rolling contact fatigue resistance for coated components
… by a 'static' Hertzian analysis. Insufficient traction forces on the contact surface between the rolling elements of a test coated bearing makes gross skidding occur, leading to rapid wear, over-heating and final failure of the test coated bearing. The LSO fatigue life of the test coated …
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