Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"traction force microscopy"”.
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Applications of Traction Force Microscopy in Measuring Adhesion Molecule Dependent Cell Contractility
… elastic modulus substrates for single cell traction force microscopy studies. The first section describes the use of EDC/NHS chemistry to convalently link microbeads to the hydrogel matrix for the purpose of performing long-term traction force studies (7 days). The final study uses the C2C12 …
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Harnessing the Regenerative Characteristics of Meniscus Superficial Zone Cells
… assays, mechanosensation analysis, and traction force microscopy. After, the matrix deposition potential of superficial cells was assessed on electrospun nanofibers. Here, we show at the transcriptional level that superficial cells are most similar to those of the meniscus body, while …
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E-cadherin mechanotransduction beyond cell-cell junctions
… modified Magnetic Twisting Cytometry (MTC) and Traction Force Microscopy (TFM) coupled with fluorescence imaging. A major focus of Chapter 2 is the role of a cadherin-associated protein α-catenin in force transduction. MTC and TFM studies using α-catenin knockdown cells and knockdown cells …
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Triggerable ligand presentation using caged-RGD
… in situ using confocal-based live cell microscopy to investigate real-time vinculin recruitment and cell traction force generation. These studies represented the first demonstration of triggerable adhesive ligand presentation in vivo and demonstrated the utility of caged-compounds for …
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Upstream Migration Under Shear Flow: Force Generation and Molecular Regulation
… Despite the centrality of mechanical force to this process, the forces upstream-migrating cells generate at the cell-substrate interface have not been directly measured during sustained upstream migration, and the molecular machinery responsible for producing them remains …
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Patterned substrates for cell culture with electrohydrodynamic jet printing
… substrates were shown to be compatible with traction force microscopy (TFM). These represented preliminary results for a larger study to evaluate how microenvironmental stiffness and geometry influence cytoskeletal contractility and ultimately bone formation. Finally, a novel method was …
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Aging Changes Cell Mechanics and Dynamics with a Backbone of Cytoplasmic Crowding
… particle fluctuation, optical tweezers, and force spectrum microscopy, we demonstrate that aging halves motion inside the cytoplasm due to doubled stiffness, while active forces remain statistically similar. In addition, we take tomograms of cell refractive index that indicate a denser …
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Microfabrication Approaches for Understanding the Role of Vascular Mechanics in Progressive Diseases
… functional contractility using a single-cell traction force microscopy technique and developed a thin-walled arterial model for growth and remodeling response to mechanical perturbations. We found that amyloid beta induces a reduction in vascular smooth muscle cell mechanical output. We …
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Dissecting combinatorial microenvironmental regulation of cell fate and function using a multi-modal arraying platform
… hybridization and, crucially, cell-generated forces using traction force microscopy (TFM); and the deconvolution of signaling via cell–cell (ligand–receptor) interactions by combining cell-extrinsic ligand presentation with cell-intrinsic ligand knockdown. I delineate the use of this platform …
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Cancer metastasis and elasticity of microenvironment
… of a finite-element-method-based cell traction force microscopy (TFM) technique to estimate the traction forces produced by multiple isolated living cells as well as cell clusters (Chapter 9).
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The role of tissue viscoelasticity in axon guidance during Xenopus brain development
… project, I developed a method based on atomic force microscopy (AFM) to measure the viscoelasticity of biological tissues (in vivo Xenopus brain and ex vivo rodent spinal cord tissue). To investigate the influence of viscoelasticity on axon guidance, I first investigated the mechanical …
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Investigating Mechanotransduction and Mechanosensitivity in Mammalian Cells
… that cells are also influenced by the mechanical forces and mechanical properties of the local microenvironment. Extracellular forces causing cellular deformation can originate from many sources, such as fluid shear stresses arising from interstitial or blood flow, mechanical stretching during …
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Investigating the role of geometry, mechanics, and microenvironmental cues on liver progenitor cell differentiation
… Here I characterize how peak cell to substrate traction stresses at the island periphery, which is also predicted by mechanical models, coincident with peak biliary fate. Building from this work, I next describe the development of a hydrogel-based microwell platform, which I used to produce …
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Cytotoxic T lymphocyte biology under the microscope
… biology. Throughout these different projects, microscopy has played an important part. Seeing really is believing, and the versatility of microscopic approaches is hopefully on full display throughout this thesis. High resolution live microscopy of individual CTL:target conjugates is perhaps …