Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 4 of 4 for “"tracrRNA"”.
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Modulation of the CRISPR-Cas9 Immune Response
… in the genome of infecting phages, and the tracrRNA to bind the promoter of cas genes and repress their transcription. We previously isolated a Cas9 mutant carrying the I473F substitution that enhanced spacer acquisition by 2-3 orders of magnitude, leading to a fitness cost due to higher …
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A Cloning-Free Recyclable System for CRISPR-Cas9 Mediated Mutant and Reversion Construction in Candida albicans Clinical Isolates
… with universal transactivating CRISPR RNA (tracrRNA) to form single guide RNA (sgRNA) that direct Cas9 to the target gene as part of the ribonucleoprotein (RNP) complex. To supplement the SAT1-flipper plasmid, we constructed an isogenic plasmid containing a C. albicans-optimized Hygromycin B …
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Utilizing the IN4MER CRISPR/CAS12A Multiplex Knockout Platform To Investigate Synthetic Lethality in the Human Genome
… direct repeats (20 nucleotides), instead of tracrRNA (76 nucleotides) in Cas9 systems, thus freeing up space to include more guides in each array and supports multiplexing. In the second part of this dissertation, I describe our multiplex Cas12a IN4MER platform and screening results, …
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Discovery of novel systems for genome editing
… S15 and the trans-activating CRISPR RNA (tracrRNA) or a single guide RNA (sgRNA) reduces, but does not abrogate, off-target integration for type V CASTs. These results provide a possible mechanism for how CASTs horizontally transfer to new hosts. More broadly, this work will guide further …