Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"toxin-antitoxin systems"”.
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Bacterial defense islands: CRISPR-Cas and toxin-antitoxin systems /
… developed a set of DNA and RNA-targeting systems to combat their invaders – bacteriophages. Fundamental research on these resistance mechanisms led to the discovery of molecular tools for genetic engineering, such as restriction enzymes, bacterial argonautes and CRISPR-Cas systems. …
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Toxin-antitoxin systems in bacteria : targets, mechanisms, and specificity
Toxin-antitoxin (TA) systems are genetic modules widely present on bacterial chromosomes. These systems comprise a toxin and cognate antitoxin that are encoded together in an operon; normally, the toxin and antitoxin are synthesized and form a non-toxic complex. Under times of stress, however, the …
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Endoribonuclease toxin-antitoxin systems in bacteria : targets and growth inhibition
Toxin-antitoxin (TA) systems are widely-distributed genetic modules that can reversibly inhibit the host bacteria's growth. Both toxin and antitoxin are encoded together on an operon and the antitoxin directly binds the toxin, preventing its activity. Under stressful conditions, the antitoxin may …
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Suitability of RelBE and MazEF toxin-antitoxin systems as antibacterial targets
Toxin–antitoxin (TA) systems are unique modules that effect plasmid stabilization via post-segregational killing of the bacterial host. The genes encoding TA systems also exist on bacterial chromosomes, and it has been speculated that these are involved in a variety of cellular processes. Interest …
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Predicting Toxin-Antitoxin Systems Responsible for Host-Induced Persistence of Burkholderia pseudomallei
… the effects of antibiotics and host defenses. Toxin-antitoxin systems (TAS) have been identified as important modulators of persistence and targeting them provides an avenue for new drug development to reduce chronic infection. When investigating TAS, the predominant issue is the presence of a …
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Investigation of the YefM-YoeBSa toxin-antitoxin systems as novel antibacterial targets
… of new antibacterial compounds. Bacterial toxin-antitoxin (TA) systems consist of a toxin capable of arresting cell growth and an antitoxin that binds to and inhibits the toxin under normal circumstances. Cellular stress causes a shift in the toxin/antitoxin ratio to favor the free toxin, …
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The prevalence of toxin-antitoxin systems and their tractability as novel antimicrobial targets
… genes, often possess plasmid maintenance systems to ensure their persistence and inheritance in a bacterial population. One example is the proteic toxin-antitoxin (TA) system, consisting of a toxin and an antitoxin encoded in a single operon. When expressed, the antitoxin binds to and …
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The genetic landscape of protein-protein interaction specificity
… of protein interfaces. Using bacterial toxin-antitoxin systems as a model, we use a variant of deep mutational scanning to dissect the positive and negative contributions of antitoxin residues that dictate toxin specificity. By screening a combinatorially complete library of antitoxin …
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The Biology and Biochemistry of PhoH2 proteins
… comprise the toxic component (VapC) of VapBC toxin antitoxin systems, in which the PIN domain is coexpressed as part of an operon with an inhibitor (VapB). In the remaining cases where PIN domain proteins can be found such as PIN PhoH domain fusions, these cases remain unexamined. This thesis …
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Regulatory RNAs at the Heart of Sugar Metabolism: New Mechanisms and Novel Discoveries
… RNA candidates, including various long 5`-UTR, toxin-antitoxin systems, prophage-encoded RNAs, and several developmentally regulated small RNAs. Their functions are still under investigation. Collectively, our studies provide important insights into the different aspects of bacterial physiology, …
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Phage host range and definition of genes implicated in Type III toxin-antitoxin-mediated abortive infection
… (phages) and have evolved a range of anti-phage systems to defend themselves. One of these systems is termed abortive infection (Abi) where, upon phage infection, an Abi system may be activated which initiate a bacteriostatic or bactericidal response. While the infected bacteria do not obviously …
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Evolution and engineering of protein-protein interactions
… has restricted the evolvability of two-component systems. I also characterized the effects of environmental context constraints, specifically temperature, on the sequence space relevant to two-component system function. This revealed generally conserved sequence-function landscapes across …
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Time to Diagnosis and Persistence: The Two Major Determinants of Effective Tuberculosis Control
… endemic Rangipo strain. The role of VapBC toxin-antitoxin systems in M. tuberculosis has been the subject of great interest recently, due to their expanded number in the genome and links with virulence and the regulation of cell growth in response to environmental stress. Their ability to …
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Structural and functional characterization of a Xanthomonas Type III effector
… similar to the Zeta-epsilon family of toxin: antitoxin systems from the human bacterial pathogen Streptococcus pyogenes. AvrRxo1 and Arc1 have toxin: antitoxin-like activity in bacteria, and the toxin activity of AvrRxo1 is required for its virulence function in planta. These findings …