Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"toxin-antitoxin"”.
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Bacterial defense islands: CRISPR-Cas and toxin-antitoxin systems /
… of type I CRISPR-Cas and CRISPR-Cas associated toxin-antitoxin systems. The first part of this thesis focuses on fundamental aspects of multiprotein class 1 type I-E and I-F CRISPR-Cas effector complexes aiming to understand molecular mechanisms of target recognition and degradation using a …
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Toxin-antitoxin systems in bacteria : targets, mechanisms, and specificity
Toxin-antitoxin (TA) systems are genetic modules widely present on bacterial chromosomes. These systems comprise a toxin and cognate antitoxin that are encoded together in an operon; normally, the toxin and antitoxin are synthesized and form a non-toxic complex. Under times of stress, however, the …
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Endoribonuclease toxin-antitoxin systems in bacteria : targets and growth inhibition
Toxin-antitoxin (TA) systems are widely-distributed genetic modules that can reversibly inhibit the host bacteria's growth. Both toxin and antitoxin are encoded together on an operon and the antitoxin directly binds the toxin, preventing its activity. Under stressful conditions, the antitoxin may …
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Suitability of RelBE and MazEF toxin-antitoxin systems as antibacterial targets
Toxin–antitoxin (TA) systems are unique modules that effect plasmid stabilization via post-segregational killing of the bacterial host. The genes encoding TA systems also exist on bacterial chromosomes, and it has been speculated that these are involved in a variety of cellular processes. Interest …
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Predicting Toxin-Antitoxin Systems Responsible for Host-Induced Persistence of Burkholderia pseudomallei
… the effects of antibiotics and host defenses. Toxin-antitoxin systems (TAS) have been identified as important modulators of persistence and targeting them provides an avenue for new drug development to reduce chronic infection. When investigating TAS, the predominant issue is the presence of a …
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Investigation of the YefM-YoeBSa toxin-antitoxin systems as novel antibacterial targets
… of new antibacterial compounds. Bacterial toxin-antitoxin (TA) systems consist of a toxin capable of arresting cell growth and an antitoxin that binds to and inhibits the toxin under normal circumstances. Cellular stress causes a shift in the toxin/antitoxin ratio to favor the free toxin, …
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The prevalence of toxin-antitoxin systems and their tractability as novel antimicrobial targets
… bacterial population. One example is the proteic toxin-antitoxin (TA) system, consisting of a toxin and an antitoxin encoded in a single operon. When expressed, the antitoxin binds to and neutralizes the toxin. However, if during cell division a plasmid-free daughter cell arises, the labile …
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The role of a toxin-antitoxin system in the arms race between bacteria and phage
… race between phages and their bacterial hosts. Toxin-antitoxin (TA) systems are widespread phage defense systems in bacteria comprising a bacteriostatic or bactericidal toxin and an antitoxin that normally keeps the toxin inactive. Following phage infection, the toxin is activated, or liberated …
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Phage host range and definition of genes implicated in Type III toxin-antitoxin-mediated abortive infection
… bacterial population benefits. The Type III toxin-antitoxin systems have previously been shown to be involved in Abi, however the mechanisms through which these systems are activated are still poorly understood. A common approach to reveal the phage product involved in triggering these …
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Differential Expression Analysis of Type II Toxin-Antitoxin Genes of Pseudomonas aeruginosa PAO1 under Different Environmental Conditions
… activities and physiological functions. Cognate toxin and antitoxin (TA) pairs, which are transcribed simultaneously from the same or different operons within the bacterial chromosomes or plasmids, play an important role for bacterial survival during stressful growth environments. Pseudomonas …
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Targeting infectious species by Structure-guided Fragment-based Drug Discovery and in silico approach
… persister phenotypes seem rather unclear but Toxin-antitoxin (TA) complexes have been reported to play a significant role in triggering persister phenotype. In this thesis, novel and validated targets are exploited using fragment-based drug discovery (FBDD) and in silico approaches to design …
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Acinetobacter baumannii toksino-antitoksino sistemos CheTA antitoksino veikimo mechanizmo paieška /
… of Activity of Acinetobacter baumannii Toxin-antitoxin System Antitoxin Acinetobacter baumannii is a gram negative opportunistic pathogen which causes variety of nosocomial infections. Due to the ability to persist in clinical environment and rapidly acquire antibiotic resistant …
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Structural and functional characterization of a Xanthomonas Type III effector
… similar to the Zeta-epsilon family of toxin: antitoxin systems from the human bacterial pathogen Streptococcus pyogenes. AvrRxo1 and Arc1 have toxin: antitoxin-like activity in bacteria, and the toxin activity of AvrRxo1 is required for its virulence function in planta. These findings …
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Evolution and engineering of protein-protein interactions
… to inform the design of protein binders to toxin-antitoxin systems. These binders are selective in their interactions with toxin homologs, and inhibit toxin-antitoxin-mediated bacterial anti-phage defense activity, suggesting their potential use in clinical phage therapy applications. Taken …
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Construction of inducible expression systems for controlled transfer of Agrobacterium tumefaciens T-DNA in plant transient expression systems /
… virulence factor virE2 and expression of toxin pemK and antitoxin pemI from bacterial toxin-antitoxin (TA) module pemIK in Agrobacterium that provides for strictly regulated T-DNA transfer from agrobacteria to model plants Nicotiana benthamiana. The study provided detailed information …
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High-Throughput Screening Development Using Photonic-Crystal Optical Biosensor
… that are DNA sequence-dependent (the bacterial toxin-antitoxin system MazEF), and those that are DNA sequence-independent (the human apoptosis inducing factor (AIF)). The PC technology was further utilized in a screen for inhibitors of the AIF-DNA interaction, and through this screen aurin …
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Anti-phage defense as a driver of molecular innovation
… of the anti-phage defense system CmdTAC, a toxin-antitoxin-chaperone system that recognizes a viral structural protein to activate a novel mRNA ADP-ribosyltransferase, thereby halting infection. Next, I examined the diversity and distribution of anti-phage mechanisms encoded by E. coli …
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The genetic landscape of protein-protein interaction specificity
… of protein interfaces. Using bacterial toxin-antitoxin systems as a model, we use a variant of deep mutational scanning to dissect the positive and negative contributions of antitoxin residues that dictate toxin specificity. By screening a combinatorially complete library of antitoxin …
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Phenotypic variation in Photorhabdus temperata K122
… csrB. Other genes, including hipB (part of a toxin/antitoxin system) and sdiA a LuxR type regulator have also been identified as having potential roles in phenotypic variation. A broad range of other genes that influence phenotypic variation either directly or indirectly have also been …
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