Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"toluene dioxygenase"”.
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The production and synthetic utility of the dioxygenase-derived metabolites of substituted aromatics
The substrate scope and selectivity of toluene dioxygenase overexpressed in E.coli JM109 (pDTG601A) was investigated with series of ortho-halobenzoates and para-substituted arenes. Palladium-catalyzed carbonylation methodology was developed to convert halogenated cis-dihydrodiol metabolites to the …
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Applications of dihydroarenediols to chemoenzymatic synthesis : approaches to total synthesis of morphine alkaloids
… recombinant organism over-expressing the enzyme toluene dioxygenase. Included in this study were bromothioanisoles, dibromobenzenes, and cyclopropylbenzene derivatives. The products of oxidation were characterized by chemical conversion to known intermediates. The synthetic utility of one of …
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Two enantiodivergent syntheses of balanol and the chemoenzymatic synthesis of oseltamivir
… the steric and functional limitations of the toluene dioxygenase-mediated oxidation of benzoate esters. The metabolite derived from ethyl benzoate was employed in a formal synthesis of oseltamivir. Finally, several synthetic approaches to oseltamivir and its analogs are presented, each …
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Chemoenzymatic Formal Total Syntheses of Tetrodotoxin and an Approach to Daphenylline
… and Sato were prepared. Key steps included toluene dioxygenase-mediated dihydroxylation of either iodobenzene or benzyl acetate and a [4+2] hetero-Diels-Alder cycloaddition/Kornblum–DeLaMare rearrangement sequence to construct a common enone intermediate. The resulting key enone was …
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Development of oxygenase biocatalysts for the production of synthetically useful cis-diol compounds
… Rieske non-heme iron oxygenases, such as toluene dioxygenase (TDO), are versatile synthons for chiral, asymmetric synthesis. The cis-dihydroxylation of ortho- and meta-substituted phenols by TDO produce cyclohexenone cis-diols. The keto group of cyclohexenone cis¬-diol tautomers increases …
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ACCELERATING WHOLE-CELL BIOCATALYSIS BY ENHANCING OUTER MEMBRANE PERMEABILITY
… the reaction rate. For reaction rates of toluene dioxygenase (TDO)-catalyzed reactions, a dramatic increase of up to six fold was observed with the lipoprotein mutant for each of the three small, hydrophobic substrates tested. Mutations in either the LPS or in the Braun's lipoprotein are …