Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"tnseq"”.
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Whole-genome transposon mutagenesis to elucidate the genetic requirements for vitamin B12 biosynthesis and assimilation in mycobacteria
… (Tn) mutagenesis and next generation sequencing (TnSeq) was applied in M. smegmatis ΔmetE, a gene-deletion mutant in which the cobalamin-independent methionine synthase is inactivated, rendering the cobalamin-dependent isoform, MetH, essential for viability. Following growth of the metE mutant in …
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Population Sequencing for Studying Natural and Artifcial Variation in C. elegans
… our method, C. elegans transposon sequencing (CeTnSeq). We have</p><p>constructed a new Mos1 transposon mutator strain that is more convenient to use</p><p>than the existing strain and allows for extra-chromosomal insertions to be degraded</p><p>by restriction digest. My preliminary results show …
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Identification and Analysis of Germination-Active Proteins in Bacillus Spores
… in a GerA-dependent manner were identified via TnSeq. Chemical cross-linking of dormant spores was implemented to characterize protein interactions leading to stabilization and activation of an important enzyme that contributes to cortex degradation in stage II of germination. The presented …
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Identificação e caracterização de genes de Herbaspirillum necessários para interação patogênica e não-patogênica com gramíneas e identificação de genes de Sorghum bicolor envolvidos na resistência a doença da estria vermelha
… viridis e H. seropedicae através da técnica de TnSeq, bem como o comportamento desta bactéria em diferentes condições de cultivo. Diversos genes candidatos foram identificados e mostram possíveis vias pelas quais planta e bactéria interagem. Sendo estas duas espécies de Herbaspirillum …
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Survival of Salmonella in the macrophage phagosome
… Typhimurium. To this end I utilized differential TnSeq, a novel genome wide approach, to identify genetic interactions with sodCI. This involved generating over 40,000 random transposon mutants in a wild type and sodC background, passaging those mutants through an intraperitoneal mouse infection …
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Barcoded Transposon Directed Insertion-site Sequencing (TraDIS); a tool to improve our understanding of the functional genomics of Streptococcus equi subsp. equi
Strangles, caused by Streptococcus equi (S. equi) remains the most frequently diagnosed infectious disease of horses and is a cause of significant welfare and economic cost. Vaccine research has been limited by the time taken to make mutations in individual genes to determine their role in the …
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Inquiries into the Mycobacterial Caseinolytic Protease System
… Previous transposon insertion sequencing (TnSeq) screens, in conjunction with our laboratory's work on mycobacterial genetic vulnerability, identified the caseinolytic protease (Clp) system, a central component of the proteostasis network, as essential and highly vulnerable in Mtb. As an …