Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"tmRNA"”.
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A SYNTHETIC TMRNA PLATFORM FOR ELUCIDATION OF BACTERIAL PROTEOME REMODELING UNDER STRESS
… ribosome rescue system, transfer-messenger RNA (tmRNA), that rapidly resolves stalled translational complexes and tags the incomplete polypeptides for degradation. Characterization of these tmRNA-tagged polypeptides could reveal previously unknown aspects of the bacterial stress response. To …
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The application of tmRNA as a marker molecule in bacterial diagnostics using microarray and biosensor technology
… üksteisest eristamisel. Pikemalt tutvustatakse tmRNA molekule, mida kasutatakse markerjärjestusena käesoleva doktoritöö raames välja töötatud diagnostiliste meetodite puhul. tmRNA on kõikides bakterites leiduv, keskmiselt 300-400 nukleotiidi pikkune spetsiifiline RNA molekul, mis abistab rakus …
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Transfer rna dynamics and transfer-messenger rna accommodation in bacterial ribosomes at the single-molecule level
… RNA (tRNA) dynamics and transfer-messenger RNA (tmRNA) accommodation inside ribosomes. In the first project of this thesis work, structural characterization of the tRNA-like domain of tmRNA (TLD) in complex with SmpB protein was carried out, and the results reveal no change in the global …
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Structural studies of trans-translation
… bacteria. Two factors, transfer-messenger RNA (tmRNA) and small protein B (SmpB), form a complex that rescues the ribosome by terminating translation and releasing the ribosome from the mRNA message. In vitro biochemistry in conjunction with cryo-electron microscopy (cryo-EM) was used to …
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GENOME-WIDE IDENTIFICATION OF RNA SENSORS IN PROKARYOTES AND EUKARYOTES
… new structural motifs. We identified a precursor tmRNA that acts as an RNA sensor for FMN to facilitate tmRNA maturation as well as an entirely new class of eukaryotic RNA sensors that recognize vitamin B2 (FMN) to control gene expression. This collection of RNA sensors expands our understanding …
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Configurable and Up-Scalable Microfluidic Life Science Platform for Cell Based Assays by Gravity Driven Sequential Perfusion and Diffusion
… first demonstrating an integrated microfluidic tmRNA purification and real time nucleic acid sequence based amplification (NASBA) device. The device is manufactured using soft lithography and a unique silica bead immobilization method for the nucleic acid micro purification column. The …
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Rickettsia prowazekii and its ‘Junk DNA’: The Identification and Characterization of Small RNAs
… kingdom-wide sRNAs (6.5S, 4.5S, RNaseP_bact_a, α-tmRNA). Three novel sRNAs and the conserved sRNAs were confirmed using either Northern blot analysis or RT-PCR. Transcriptional start sites were determined using RLM-RACE and secondary structure predicted for five novel sRNAs and 6S RNA. The third …