Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 42 for “"tissue-specific expression"”.
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Rat angiotensin-converting enzyme : tissue specific expression during pharmacological inhibition
… to enable examination of ACE mRNA and protein expression during enzyme inhibition with enalapril. Rat lung ACE mRNA increased by 156% (p<0.05) and ACE protein doubled within 3 hours of administering a single dose of enalapril. Testicular ACE mRNA also increased by 300% (p<0.05) within 2 hours …
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Tissue-Specific Expression and Steroid Hormone Regulation of Human Gonadotropin -Releasing Hormone (hGnRH) Gene in Placental Cells (JEG -3 cells)
… model, I investigated the mechanisms behind the tissue-specific expression and steroid hormone regulation of the hGnRH gene in the human placenta. The hGnRH upstream promoter was found to be functionally active in JEG-3 cells. The DNA sequence responsible for functioning of the upstream promoter …
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Characterization of an aryl hydrocarbon receptor from a cetacean : an approach for assessing contaminant susceptibility in protected species
… halogenated aromatic hydrocarbons (HAH) in their tissues, but little is known about the effects of such burdens on cetacean health. 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and related HAH cause toxicity via activation of the aryl hydrocarbon receptor (AHR), a member of the bHLH-PAS family of …
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Isolation, Culture and Cryopreservaion of Neural Stem Cell
… the second intron is sufficient to direct tissue specific expression of the nestin gene in developing embryos. In this study, we investigated whether the second intron of the rat nestin gene is sufficient to confer tissue-specific expression of the gene in neural stem cells in vitro and in …
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A C. elegans histone methyltransferase promotes spermatocyte gene expression, spermatid production and fertility
To better understand the tissue-specific regulation of chromatin state in cell-fate determination and development, we defined the tissue-specific expression of all 36 lysine methyltransferase (KMT) genes by endogenous mRNA detection in C. elegans. We found that most KMTs are expressed in only one …
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Computational prediction of RNA-based gene regulatory mechanisms in human and Tetrahymena
… in ciliates and developmental timing and tissue differentiation in plants and animals. Here we present a computational study into the function of two distinct classes of sRNAs. In the first section, we examine an uncharacterized class of sRNAs isolated from the ciliate Tetrahymena …
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A Census of Human RNA-Binding Proteins and Characterization of the DEDDh RNA Exonuclease NEF-sp in the 3' End Maturation of 28S Ribosomal RNA
… their evolutionary patterns, abundance, and tissue-specific expression. Coregulated gene expression during developmental processes often gives novel insights into regulatory pathways and components. Furthermore, I showed that by classifying RBPs into their main regulatory RNA pathways we can …
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An Investigation Into Splicing Variation and Expression Patterns of Insulin-Like Peptide 4 (ILP4) Within the Drosophila Genus
… identified is significant variation in sex specific ILP4 expression patterns across the Drosophila genus. Future research is called for to explore tissue-specific expression and the observed functional consequences of sequence variations. By investigating this splicing variation and …
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Molekulare Markierungsstrategien für die optische und magnetresonanztomographische zelluläre Visualisierung
… based strategy was implemented and modified. Overexpression of human heavy chain ferritin in combination with firefly luciferase in glioma cells led to a cell specific internal MR contrast in combination with bioluminescence detection. For efficient transduction a lentiviral system was used. …
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3D Chromatin Structure and Gene Regulation: Integrated Genome Structure, Transcriptomics and Epigenetics
… of the genome is essential for regulating gene expression, replication, and stability. Chromatin folding mediates enhancer–promoter communication, but bulk Hi-C data obscure single-cell variability, while sparse single-cell Hi-C limits structural resolution. This dissertation develops …
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Characterization of the expression profile of polyamine biosynthetic genes (Spermidine synthase) and polyamine metabolic regulation in arabidopsis
… the regulation of polyamine biosynthetic gene expression and polyamine metabolism. The organ/tissue specific expression patterns of two genes encoding the polyamine biosynthetic enzyme spermidine synthase ( AtSPDS1 and AtSPDS2) were studied in Arabidopsis at different developmental stages using …
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Roles of Neurogenin and Geminin in Vertebrate Neurogensis
… consensus binding sites and drove neural tissue-specific expression in transgenic Xenopus embryos. We used the Neurogenin binding consensus and a computational approach to predict additional target genes involved in neural development at a genome-wide level. Taken together, these data …
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Characterization of lin-42/period transcriptional regulation by the Ikaros/hunchback-family transcription factor ZTF-16 in Caenorhabditis elegans
… poorly understood. The structure, function and expression of lin-42 are all similar to period. Therefore, we are studying lin-42 regulation and function during C. elegans larval development as a model for understanding period control of mammalian stem/progenitor cell development. Previous work …
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Studies on maize beta glucosidase gene-enzyme system
… by the end product, glucose. The sporophytic specificity of Glul expression might be controlled by tightly linked cis- and trans- acting regulatory elements. One of the several null mutations, with an apparent allelism to Glul locus, (l) complements in trans when combined with normal Glul …
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Multi-omics Investigation into Alzheimer's Disease: Functional Mechanism and Early Detection
… from genome-wide association study (GWAS) and expression quantitative trait loci (eQTL) analysis. Results of this study confirmed the potential of integrative GWAS and eQTL analysis in estimating the transcriptomic changes when lack of tissue-specific expression data, and provided important …
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Small RNA studies in Drosophila melanogaster, Stylophora pistillata and Symbiodinium sp.
… RNAs (piRNAs) play a big role in regulating gene expression in cells. In my work, I focus primarily on miRNAs, which represses the expression of the mRNA targets post-transcriptionally. For Drosophila melanogaster, I predicted the tissue-specific expression of several miRNAs based on the …
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Improvement of expression of recombinant human protein C in the milk of transgenic animals using a novel transgene construct
Past studies of mammary tissue specific expression of transgenes using the murine whey acidic protein (WAP) promoter have shown widely variable, position-dependent and copy number-dependent expression. This study evaluates a series of three WAP transgenes containing the cDNA of human protein C …
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Analysis of the Role of bHLH/PAS Proteins in Aryl Hydrocarbon Receptor Signaling
… nuclear translocator (ARNT) and associates with specific cis xenobiotic response elements to activate transcription of genes involved with xenobiotic metabolism. AHR-mediated signal transduction has been evaluated thoroughly in the C57BL/6J mouse model system. This model system, however, may not …
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Modulation of Cerebral Artery Smooth Muscle BK Channel Function by Neurosteroids
… of the channel; this modulation is subunit-specific. Moreover, these accessory subunits have tissue-specific expression, such that the β1 subunit is highly expressed within cerebrovascular smooth muscle and greatly increases the open probability of BK channels. Increased BK channel open …
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Transcription regulation of the class II alcohol dehydrogenase 7 (ADH7)
… in the upper gastrointestinal tract with no expression in the liver unlike the other ADHs, and is implicated in various diseases including alcoholism, cancer and fetal alcohol syndrome. Genome wide studies have identified significant associations between ADH7 variants and alcoholism and …
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