Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"tissue tropism"”.
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Generation and Characterization of Flavivirus Receptor-Binding Domain Mutants and Chimeras
… the role of EIII in virus growth and tissue tropism, chimeric viruses were generated using the West Nile virus (WNV) infectious clone, into which EIIIs from a range of flaviviruses of increasing genetic diversity from WNV were substituted. Four versions of each chimera were generated …
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Investigation of the interplay of yellow fever virus structural protein epitopes and genetic diversity
… of the wild type (WT) strain Asibi in chicken tissue. The mechanism of attenuation of 17D is incompletely understood. This dissertation investigates the contribution of the envelope (E) protein in YFV attenuation through its role in genetic diversity, tissue tropism and recognition by WT and …
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Factors affecting replication and cross-species transmission of feline immunodeficiency virus
… of accessory protein Vif. Viral entry and tissue tropism are determined by an interaction of the viral Env glycoprotein with a cell surface receptor and a seven transmembrane domain co-receptor. The feline immunodeficiency virus (FIV) infects diverse felid species including the African …
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Murine Cytomegalovirus Encodes Proteins that Regulate Viral Late Transcription
… structure, genome organization, gene expression, tissue tropism, and clinical manifestations. In my graduate studies, I discovered the function of two novel MCMV proteins during in vitro infection. I demonstrated that ORF M79 encoded protein pM79, and ORF M92 encoded protein pM92. Both pM79 and …
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Establishment and Improvement of Preclinical Murine Models of Severe Scrub Typhus to Define Immune Signatures and Pathogenic Mechanisms
… model of hematogenous transmission that mimics tissue tropism and pathologic lesions of scrub typhus patients. Interestingly, we find this outbred model exhibits the same pro-inflammatory/Th1 skewed immune response as seen in our previously established inbred model. We further developed our …
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Factors affecting transduction efficiency of pseudotyped viral vectors incorporating alphaviral glycoproteins
… of another virus that has the desired range of tissue tropism. Such hybrid viruses are called pseudotyped viruses. Previously, we have pseudotyped Moloney Murine Leukemia Virus (MoMuLV), a retrovirus with the envelope of Ross River Virus (RRV), an alphavirus. Here, we substituted amino-acid …
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Leveraging <i>Borrelia burgdorferi</i> peptidoglycan chemistry to understand Lyme disease symptom progression
… that B. burgdorferi PG persists in host tissue and in the synovial fluid of LA patients, and the unique structure of B. burgdorferi PG may play a key role in that. As B. burgdorferi lacks the machinery to recycle shed PG fragments, the bacterium sheds nearly 50% of its cell wall every …
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DEVELOPMENT OF AVIAN PARAMYXOVIRUS VECTORED VACCINES
… in vitro, and for immunogenicity, safety, and tissue tropism in day-old specific pathogen-free (SPF) chicks. APMV-3 strain Netherlands (APMV-3 Neth.) showed the highest growth rate and GFP expression in chicken fibroblast DF-1 cells, followed by APMV-1 LaSota and APMV-3 Wisconsin. In day-old …
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Identification and Characterization of Novel Caenorhabditis Nematode Viruses
… infection localized primarily to the intestinal tissue. Strikingly, only 1 to 6 cells of the total 20 nonregenerating intestinal cells were positive by IFA for viral protein, suggesting that infection is confined to a subset of the intestine. A transcriptional profile analysis was carried out to …
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Cha-Cha-Cha: Variable Adhesive Activity of the <italic>Haemophilus</Italic> Cryptic Genospecies Trimeric Autotransporter Cha
… of Cha offers insight into the apparent tissue tropism associated with the <italic>Haemophilus</italic> cryptic genospecies. We speculate that the unique regulation of Cha adhesive activity enhances the adaptive capability of this pathogenic organism in the human host.</p>
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Host-Parasite Interrelations: Bacterial Adherence to and Invasion of Epithelial Cells
… of opacity. The role of P.II as a determinant of tissue tropism is discussed.</p>