Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"tissue slices"”.
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The Effect of Trypsin on the Respiration of Tissue Slices
… the proteolytic enzyme derived from pancreatic tissue, has long been a common agent used in tissue culture work. It is used to separate cells, either: 1. from intact animal tissue for primary explants or 2. from the bottom of culture flasks for further investigation. In fact, many workers use …
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Electrophysiological characterization of insulin secreting beta-cells in pancreatic tissue slices
In der vorliegenden Studie wurde eine Präparation pankreatischer Gewebeschnitte etabliert, um unter Bedingungen nahe in vivo, die Charakterisierung Insulin sezernierender β-Zellen, zu ermöglichen. Das Arbeitsverfahren zur Herstellung von Gewebeschnitten des Pankreas ist schnell und leicht …
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A Framework for 3D Mouse Brain Reconstruction: RNA-based Stitching of Adjacent Tissue Slices and Co-Registration of Multimodal Imaging Data
… neurons. However, SRT methods are limited to slices of tissue; therefore, computational alignment is essential to reconstruct a cohesive 3D volume while correcting for both batch effects and inherent sample variability. This thesis presents a novel framework that addresses these challenges …
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Effects of swelling on brain tissue mechanical properties by indentation
… on identifying the mechanisms underlying brain tissue swelling. Most commonly, magnetic resonance imaging techniques have been used, particularly with the development of magnetic resonance elastography, to study swelling in vivo. However, localized testing on in vitro tissue slices have become …
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Manometric Studies of Earle's Strain L Sarcoma Cells
… generalizations concerning malignant tumor tissue metabolism, and summarized from his studies that "interference with the respiration in growing cells is, from the standpoint of the physiology of metabolism, the cause of tumors. If the respiration of a growing cell is disturbed, as a rule …
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Model-based quantitative combinatorial optical microscopy for extracting clinical imaging biomarkers
… Applications include identification of tissue constituents in fixed tissue slices and classification of cell death mechanisms in a living engineered tissue sample.
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Calcium Regulation of a Slow AHP in Hippocampal Pyramidal Cells
… or hipppocalcin. In recordings in hippocampal tissue slices in vitro the KCa3.1-mediated IsAHP steadily increased over 10 min equilibration to 0 µM calcium electrolyte, but decreased upon infusing 1 µM calcium, while the KCNQ-mediated IsAHP responded in an opposite manner. KCa3.1 channels …
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The application of deep learning to nucleus images for early cancer diagnostics
… usually involves a pathologist looking at tissue slices of patients for specific features associated with cancer prognosis such as nuclear morphometric measures. However, early diagnosis remains a major challenge. Recent studies have shown that changes in fibroblast nuclei play a critical …
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Mid infrared spectroscopic imaging and tomography
… detection in prostate, breast and skin cancer tissues. These studies utilize FT-IR imaging data of thin tissue slices from several hundred patients. The segmentation algorithms and instrumentation used are described in detail. We perform a rigorous analysis of instrumentation and data so as to …
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Studies of proteins in the posterior lobe of the pituitary gland
… used to demonstrate neurophysin-like antigen in tissue slices. Initially tissue was frozen unfixed and sectioned in a cryostat prior to fixation in 95% (v/v) aqueous ethanol. subsequently an improved method was developed in which tissues were fixed in saline-formalin and embedded in paraffin and …
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The Relationship Between Resistance to the Fungal Toxin Cercosporin and Grey Leaf Spot Disease Resistance in Corn (ZEA MAYS L.)
… in the conductivity of water containing leaf tissue slices following exposure to cercosporin. Ion leakage is an indicator of cellular damage, so resistant tissue would be expected ro have a lower level of conductivity increase than susceptible tissue. Resistance to Grey Leaf Spot disease had …
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Characterization of neurite growth on a novel neurotube platform
… years of exploring electrical dimensions of tissue slices and cultures, there is still a wealth of information yet to be deciphered. In particular the role of dendrites in network formation and communication was largely overlooked while the axon was considered the principal player. Dendrites …
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Ion flow and membrane tension studies with optical tweezers and nanopipettes
… achieved. Recordings were attempted in brain tissue slices and also performed simultaneously with micro-electrode array recordings for comparison with network activity. Electrophysiological recordings from unmodified escherichia coli bacteria are also demonstrated. The outcome of this work is …
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Visualizing Cell Migration in Situ
… from the somite into the forelimb using embryo slices. New technologies were developed to stabilize slices within the microscopic field and to apply surface support along cut margins of the slice to prevent exuberant outgrowth from the slice. Muscle precursors, in somites, initiate migration …
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Regulatory Roles of Essential Amino Acids, Energy, and Insulin in Mammary Cell Protein Synthesis
… synthesis rates were examined with mammary tissue slices. A mathematical representation of the insulin and EAA effects was developed. The effects of supplementing BCAA on milk protein synthesis were investigated using nine Holstein cows, assigned to 7 d continuous jugular infusions of …
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Essential Amino Acid Regulation of Cell Signaling and Casein Synthesis in Mammary Tissue
… and to stimulate protein synthesis in mammary tissue. The<br />objectives of this research were to determine the response to Ile, Leu, Met, and Thr in<br />cellular signaling and "-S1 casein fractional synthesis rates (CFSR). An experiment was<br />developed as a composite design. The …
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Advancing three-dimensional electrophysiology: Development and evaluation of versatile platform for tailored 3D microelectrode array fabrication
… in vitro neuronal networks and acute brain slices. Planar or 2D MEAs have been the conventional standard for decades, enabling the extracellular recording and stimulation of cultured neuronal cells and tissue slices. However, the effectiveness of planar MEAs diminishes when stimulating or …
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The hormonal control of lipid metabolism in sheep
… was investigated by incubation of ovine adipose tissue slices in the presence of hormones the in vitro. The effects of insulin, catecholamines, growth hormone (GH) and insulin-like growth factors (IGFs) on lipogenesis and lipid mobilization were studied to assess their likely individual …
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Studying the glial cell response to biomaterials and surface topography for improving the neural electrode interface
… due to the development of glial scar tissue (Polikov et al., 2006; Zhong and Bellamkonda, 2008). The long-term effect of chronically implanted electrodes is the formation of a glial scar made up of reactive astrocytes and the matrix proteins they generate (Polikov et al., 2005; Seil …
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Turn-on fluorescent probes for detecting nitric oxide in biology
… of NO production using fluorescence in tissue slices of the mouse main olfactory bulb is reported. This discovery was possible through the use of a novel, celltrappable probe for intracellular nitric oxide detection based on a symmetric scaffold with two NO-reactive sites. Ester moieties …