Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"timelapse"”.
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2D timelapse and 3D fluorescence microscopy with applications to vascular tissue engineering
… and endothelial cells (EC). Two dimensional timelapse fluorescence microscopy revealed rapid alkalinization occurring in cultured human aortic SMC exposed to well defined fluid flow profiles. The response was reversible and persisted for at least 20 min after flow initiation. The magnitude of …
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Resistance Responses in Pseudomonas aeruginosa PAO1 in Response to Surface Predation by Myxococcus xanthus DK1622
… an increase in dead prey at the cellular level. Timelapse videos showed that ripple formation is inhibited at 48 hours. “Fold” deficient <em>P. aeruginosa</em> PAO1 strains clarified that <em>M. xanthus</em> is responsible for the appearance of the fold by showing complete blockage of <em>M. …
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Mechanisms of Gradient Tracking During Yeast Mating
… the cortex, directing growth toward that site. Timelapse microscopy of fluorescently-labeled polarity proteins revealed that the patch wandered around the cortex during gradient tracking. Mathematical modeling and genetic analysis suggested that fusion of vesicles near the polarization site …
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Cell Polarity Establishment in the Budding Yeast Saccharomyces Cerevisiae
… drugs Latrunculin A and B as well as high-speed timelapse microscopy of fluorescently labeled polarity proteins to characterize the assembly of the incipient bud site. </p><p>Often, ensuring that only a single axis of polarity is established is as important as generating asymmetry in the cell. …
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Harnessing the Anaphase-Promoting Complex for Targeted Protein Degradation
… using cell-based degradation assays conducted by timelapse quantitative fluorescence imaging. The study of degron combinations provides lessons on how the sequence, spacing, orientation, and number of degrons affect the rate, timing, and extent of degradation. One of the effective degron …
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Developmental Patterning and Cell Fate Specification in the Mouse Colon
… existing techniques to allow for ex-vivo timelapse imaging of the developing organ, I proposed and tested an epithelial-driven model for the formation of the stem cell niche before birth. Furthermore, by performing lineage tracing experiments, I was able to identify the mechanisms through …
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High-Throughput Single-Cell Functional and Molecular Profiling of Immune Cells in Cancer Immunotherapy
… first part of this dissertation, we implemented Timelapse Imaging Microscopy in Nanowell Grids (TIMING) to demonstrate that while CD4+CAR+ (CAR4) cells killed at slower rate, most likely due to lower granzyme B content, they benefited from apoptosis resistance compared to CD8+CAR+ (CAR8) cells. …
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Single-cell Functional Profiling of Lymphocytes for Cancer Immunotherapy
… developed and implemented a methodology based on Timelapse Imaging Microscopy in Nanowell Grids (TIMING) platform that integrates phenotypic profiling and dynamic cytokine secretion with single-cell resolution. Analysis of hundreds of human peripheral nature killer cells (NK cells) suggested that …
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A Genome-Wide RNAi Screen Identifies CDC-42 and GDI-1 as In Vivo Regulators of Invadopodia
… genome-wide RNAi screen, classical genetics, and timelapse imaging of invadopodia at the AC-BM interface, Chapter 3 presents two in vivo invadopodia regulators that function by distinct mechanisms. This is the first in vivo evidence that the RhoGTPase CDC-42 regulates invadopodia formation through …
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Investigating Transluminal Endothelial Bridging in a Microvessel-on-a-Chip
… wounds. Next, I undertook multipoint 3D timelapse microscopy of the microphysiological system which revealed the evolution of circumferentially orientated endothelial cell protrusions into transluminal bridges forming by partial delamination. I found that enhancing actomyosin tension by …
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Investigating the role of endoplasmic reticulum stress in neutrophils during tumour initiation
… PNC-associated neutrophils and proceeded with timelapse confocal live imaging from 24 to 36 hpi to monitor ER stress in these cells. Surprisingly, my results indicated subtle intensity changes indicative of ER stress in PNC-associated neutrophils, as confirmed by FACS analysis. I also generated …
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Beyond Suspension: Light and Growth Dynamics in Immobilised Algal Cultures
… model of algal biofilm dynamics, the use of timelapse microscopy and single-cell tracking within microcolonies uncovered how external matrices and exogenous nutrient supply influence cell cycle and morphology. The findings revealed spatial heterogeneity among cells within colonies, providing …
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In Vitro Models of Cellular Dedifferentiation for Regenerative Medicine
… of murine C2C12 myotubes into myoblasts. Timelapse photography, immunofluorescence, and western blot analysis support successful dedifferentiation into myoblasts. However, the inability to separate the myotubes and myoblasts prior to siRNA treatment confounded the results. This system has …
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Remote sensing of river kinematics and their expression in the modern and ancient stratigraphic record
… via analysis of modern river kinematics using timelapse satellite imagery and characterization of uniquely exposed ancient river deposits using photogrammetric and numerical modeling approaches. The research described herein focuses on three central themes: (1) the geomorphic and stratigraphic …
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Dynamic Assessment of Nk Cell Interactions With Pediatric Tumor Cells to Predict Response to Immunotherapy
… formation between the NK cells and tumor cells. Timelapse imaging of neurobastoma cells with increased sensitivity revealed that IFNγ treatment also decreased the time for NK cell encounter of tumor cells (t<sub>seek</sub>). Blocking of ICAM-1 weakened the increased sensitivity observed after …
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A Novel High Throughput Platform for Assessing Bacterial Growth and Morphology Enables Rapid Antibiotic Susceptibility Testing
… 96 agarose pads and relies on single-cell timelapse microscopy to image bacteria as they develop into microcolonies. The platform can be manufactured with a laser cutter at a low cost based on off-the-shelf parts, making it easy to adopt for other labs. A robust open-source segmentation …
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Understanding emplacement of rapidly-emplaced volume-limited lava flows from Mount Etna’s 2011-2012 eruption : a multidisciplinary study
… (1) employing long-range ground-based visible timelapse data and thermo-rheological modelling to understand and analyze the importance of different factors which influence lava flow emplacement, and (2) developing a workflow for improving the application of long-range ground-based thermal …
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Hierarchical Bayesian approaches to seismic imaging and other geophysical inverse problems
… we shift our focus and consider the problem of timelapse seismic processing, where the objective is to detect changes in the subsurface over a period of time using repeated seismic surveys. We focus on the realistic case where the surveys are taken with differing acquisition geometries. In such …
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Chemomechanics of self-oscillating gels
… state of covalently bound Ru(bpy) 3 . Using timelapse microscopy, we recorded the BZ oscillations and tracked the far from equilibrium chemical behavior exhibited by the gels. At constant system temperature, the BZ reaction induced cyclic changes in the osmotic pressure of the gel, resulting …
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At the Conflux
<p>1. At the Conflux </p><p>At the Conflux is a three part musically driven time-lapse film that tours Japan by road and rail. It is an exploration of its sprawling nocturnal cityscapes crisscrossed by thruways, its urban grid illuminated by fiery-hued highways pulsing through the city like …
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