Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"time-lapse microscopy"”.
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Quantitative analysis of axon elongation : a novel application of stochastic modeling techniques to long-term, high-resolution time-lapse microscopy of cortical axons
… for in vitro imaging of axon growth cones with time-lapse fluorescence microscopy. Image tiles are automatically captured and assembled into a mosaic image of a square millimeter region. GFP-expressing mouse cortical neurons can be imaged once every few minutes for up to weeks if phototoxicity …
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Investigation of Bacillus subtilis Sigma Factor Dynamics Using Improved Single Cell Tools
… factors, quantitative single cell snapshots, time-lapse microscopy, and microfluidics.\\ By developing an improved microfluidics setup for single cell time-lapse microscopy, as well as improved single cell analysis code, we are able to observe new sigma factor dynamics. First, we observe …
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Single-Cell Image Analysis Enables High-Throughput Phenotypic Drug Screen and Elucidates Cell-Fate Decision Principles
… cells under non-lethal dose of chemotherapy via time-lapse microscopy before, during and days after treatment. Surprisingly, while high p21 is associated with senescence at late times, we find the opposite at early times during drug treatment: most senescence-fated cells have low p21 levels, …
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Single-Cell Image Analysis Enables High-Throughput Phenotypic Drug Screen and Elucidates Cell-Fate Decision Principles
… cells under non-lethal dose of chemotherapy via time-lapse microscopy before, during and days after treatment. Surprisingly, while high p21 is associated with senescence at late times, we find the opposite at early times during drug treatment: most senescence-fated cells have low p21 levels, …
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Generation and circuit development of zebrafish retinal horizontal cells
… the nervous system. Upon completion and sometimes during neuronal migration, neurons must elaborate complex dendritic and axonal arbors and then form precise synaptic connections in the background of a plethora of possible synaptic partners. Glial cells must also establish specialized …
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Von der Form zur Richtung
… known to induce growth cone turning. By using time-lapse microscopy and release of cAMP from biological inactive caged compounds the directional response of neuronal growth cones was investigated. Intracellular gradients were induced by asymmetric release of cAMP on one side of the growth cone. …
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Cell Migration and Morphogenesis during Formation and Repair of Zebrafish Skeletal Muscle
… different compartments in vivo via 3D confocal time-lapse microscopy. I demonstrated that the epaxial and hypaxial somite regions show distinct morphogenesis that lead to the chevron-shape of the somite. Evidence is presented that shows that the somite volume does not significantly change during …
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Regulation of the Anaphase-Promoting Complex Examined at the Single Cell Level
… activity in single cells by quantitative time-lapse microscopy. I found that APC-Cdh1 is inactivated with very reliable timing, in contrast with other cell cycle events that occur with considerable variability in timing. The activity of APCCdh1 is restrained by multisite phosphorylation by …
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On the coupling of noisy processes in biology to produce functional phenotypic variability
… expression. Combining microfluidics and time-lapse microscopy we reveal multi-generation RpoS activity pulses are responsible for this heterogeneity. We next show that RpoS and growth have stochastic dynamics and are anti-correlated. With a stochastic simulation of chemical reactions …
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Dynamics of gene expression and signal transduction in single cells
… components[34]. In this work, we combined time-lapse microscopy, quantitative image analysis and fluorescent protein reporters, which allowed us to directly observe multiple cellular components over time in individual cells. In conjunction with mathematical models, we have investigated …
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Effect of Surface Chemistry and Young's Modulus on the Surface Motility of the Bacterium Pseudomonas Aeruginosa
… PDMS. These results were obtained using time-lapse microscopy of bacteria exposed to continuously flowing tryptic soy broth growth medium at 37 ⁰C. Not only are the mean speeds different, the distributions of speeds are also different: on the hydrophobic substrate, a smaller proportion of …
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Factors regulating transitions among life cycle phases in the marine pennate diatom <i>Pseudo-nitzschia multistriata</i>
… of an investigation using con focal and time lapse microscopy aimed at describing the different stages of the life cycle of this species. Results are also presented of various experiments aimed at defining the endogenous and exogenous factors that induce sexual, reproduction. Parental …
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Investigating the effect of amyotrophic lateral sclerosis-associated mutant vesicle-associated membrane protein B on axonal transport
… neurons and primarybmouse motor neurons. Using time-lapse microscopy, I showed that expression of VAPBP56S but not wild-type VAPB in neurons selectively disrupts anterograde axonal transport of mitochondria. Anterograde axonal transport of mitochondria is mediated by the microtubule-based …
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Quantitative Characterisation of Morphological and Phenotypic Changes During Microbial Cell Differentiation and Multicellular Behaviour
… by the cells of matrix mutant strains by time-lapse microscopy, and employing still-image quantitation, reveals some physical properties of the matrix components and goes some way to explain the common macro-scale morphologies of these structures described previously.
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Quantitative characterisation of single-cell circadian rhythms in cyanobacteria Synechococcus elongatus
… cell growth and division. Using quantitative time-lapse microscopy, we measured the robustness of cyanobacterial circadian rhythms with previously inaccessible precision. We observed extremely robust timekeeping under various deterministic light environments. Using clock mutants, we revealed …
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Propagation of engineered cellular properties to colony scales in bacterial populations
… in natural multicellular systems. A range of microscopy techniques and image processing tools were established to measure cellular properties and cellular arrangements, as well as the dynamics of colony growth. The experimental work was closely integrated with biophysical computational …
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Mitotic Exit Control in Budding Yeast: Regulators and Dynamics
… mitotic cyclin Clb2. Using quantitative time-lapse microscopy, we demonstrate an intrinsic oscillatory module controlling Cdc14 localization. This autonomous Cdc14 release oscillator functions at constant cyclin-Cdk levels by titrated introduction of undegradable Clb2, and at …
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Detection and tracking of cells and focal adhesions in microscopy images
… of cell function and structure. Modern microscopy and imaging techniques have enabled cell biologists to observe and record cell processes in detail, thus generating high volumes of imaging data. Meaningful conclusions can only be drawn from such big datasets using automated quantitative …
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Riboswitch regulation of methionine metabolism and vitamin B12 uptake in mycobacteria – implications for drug susceptibility and pathogenesis
… of genetics, gene expression analysis, live-cell time-lapse microscopy and targeted metabolite and protein analysis via mass spectrometry (MS) was then employed to investigate cobamide riboswitch-dependent regulation of methionine biosynthesis in M. smegmatis. Results indicated that, in wild-type …
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