Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"time-lapse imaging"”.
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Többfalu karbon nanocsövek cito-és genotoxikológiai vizsgálata humán limbális sejteken fluoreszcens mikroszkóppal és time-lapse imaging videomikroszkópiás módszerekkel
A szakdolgozatomban az MWCNT egyik fajtájával, a sMWCNT-vel foglalkoztam.Sejt szinten végbemenő változások vizsgálata volt az elsődleges célom. Azt vizsgáltam, hogy milyen változásokat okoz sejtmaganyag szinten a szén nanocső, illetve ez hogyan befolyásolja a kromatin kondenzálódási …
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In Vitro Long Term Time-Lapse Imaging Studies Of The Mcf-10 Mammary Cancer Cell Progression Model Using A Custom Incubator Microscope
Time-lapse imaging of cells has been historically limited to a few hours because of the difficulty associated with maintaining the appropriate physiological pH and temperature of the cells during prolonged imaging. Carbon dioxide produced by cellular respiration changes the pH of the medium, and …
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The dynamics of anti-tumour responses generated by chimeric antigen receptor-modified immune effector cells
… one to study the fate of individual cells over time. Therefore, the aim of this thesis was to apply flow cytometry, time-lapse imaging and singlecell tracking to characterise the dynamics of CART and tumour cell interactions in vitro. The utility of time-lapse imaging and single-cell tracking …
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Characterization of Stem Cell Turnover In A Living Epithelial Bilayer
… population numbers in a bilayered epithelium. Time-lapse imaging and electron microscopy experiments reveal that dying cells are rapidly cleared as nearby basal cells engulf apoptotic debris. Remaining basal cells subsequently divide to replace lost cells, allowing for conservation of tissue …
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Deformation mapping of fibronectin fibrils using digital image correlation
… of the earliest ECM proteins to be assembled. Time lapse imaging techniques show that the deposition of fibronectin by osteoblasts is a highly dynamic process, in which the forming fibronectin fibril network is continually stretched, contracted and moved via the activity of motile cells. This …
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Generation of human inner ear organoids from pluripotent stem cells and an organ-on-chip platform
… inserts) to enable high-resolution time-lapse imaging of the inner ear vesicles. This provides a useful tool to study inner ear diseases and aids in the discovery of safe and precise therapies.
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Genetic Control of Root System Architecture in Rice
… usage. </p><p>Our lab has developed a gel-based imaging and phenotyping system to facilitate genetic mapping of root traits in rice. Using this, we identify a mutation in a gene encoding a putative rice histidine kinase (OsHK1) that results in plants with increased seedling root depth. Using …
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Investigating how ubiquitin-mediated proteolysis of AURKA contributes to its activity in the cell cycle
… by destruction. These, in combination with time-lapse imaging, show that inactivation of AURKA is identical in wild-type and FZR1KO cells, despit¬e the difference in protein levels between the two cell lines. I demonstrate that the timing of AURKA inactivation is regulated via the …
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Investigating the consequences of chromosome abnormalities arising during pre-implantation development of the mouse
… developmental outcome of mosaic embryos. Time-lapse imaging of pre-implantation development revealed that cells with chromosome abnormalities were progressively depleted during blastocyst maturation; inner cell mass (ICM) cells exhibited higher rates of apoptosis, while in the …
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Multimodal intravital imaging of tissue structure and cell dynamics in skin using integrated optical coherence and multiphoton microscopy
… and application of multimodal optical imaging methods for providing enhanced visualization of living skin. In vivo imaging of skin is of great interest for research applications to study fundamental aspects of skin biology as well as for clinical applications to potentially enable …
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Methods and technologies for high-throughput and high-content small animal screening
… their immobilization for high-resolution imaging and manipulation. Here we present methods to enable HTS and HCS of the nematode Caenorhabditis elegans (C. elegans). First we present microfluidic technologies to rapidly isolate, immobilize, image and manipulate individual animals. These …
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Gastrointestinal (GI) Motility is Unaltered by Feeding in Zebrafish
… FITC-dextran and GI motility was measured using time-lapse imaging and image analysis techniques. Motility was examined in the anterior and the posterior regions of the GI tract. No differences were observed in fish standard length, a developmental marker. The total number and distance of …
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Development of Novel Methods to Study Host-Microbe Interactions In The Larval Zebrafish Gastrointestinal Tract
… dynamic events in the intestine via time-lapse imaging. In the first part of this work, I discuss our efforts to optimize the parameters of a foodborne infection assay using paramecia as a vehicle. This method provides an effective, high-throughput alternative to infection via …
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Strain Estimation of Fibronectin Fibrils Using Two Dimensional Digital Image Correlation
… into how the bone matrix is formed. Live cell imaging of fibronectin assembly in living osteoblasts helps in understanding the kinematics of its assembly, which is a highly dynamic process. Fibronectin assembly was imaged in 2T3 osteoblasts using time lapse imaging over a 48 hour period. This …
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Quantification of Aggregate Formation in Prochlorococcus
… The aggregate formation was tracked via hourly time-lapse imaging of Prochlorococcus grown under constant light axenically, and xenically with Alteromonas sp. EZ55, and after environmental stressors of dark incubation, heat shock, and EDTA- addition. An algorithm was developed to measure the …
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Approaches for Microfluidics-Enabled, Bacterial, Single-Cell Synthetic Biology
… as collective behaviours over long periods of time. Therefore, for successful biocomputing of living cells it is crucial to design, build and test engineered cells at single-cell resolution, with time-dependent behaviour in mind. This is possible through microfluidics-enhanced time-lapse …
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The Protective Effects of Memantine On The Morphology and Activity of Irradiated Dendritic Spines
… in proportion stubby and branched spines. Time-lapse imaging was used to characterize the alterations of the dendritic spines of cultured cortical neuron upon radiation treatment, in real time, with or without memantine. The total activity was immediately increased in the neuron group …
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Cell Size Control And Asymmetric Cell Fates In Start Of The Saccharomyces cerevisiae Cell Cycle
… of cell size control, by combining single-cell time-lapse imaging of fluorescently labeled cells and rigorous mathematical analysis. This allowed us to quantify the contributions of size control and molecular noise to temporal variability of the G1 phase. Comparing wild-type and mutant strains …
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Digital Holographic Microscopy for High-throughput Analysis of Tumour Cells
visible, and in time-lapse imaging, light levels can be harmful for cells. These routines can affect the behaviour of cells and introduce unwanted artefacts in the captured images. Digital holographic microscopy(DHM)enables stain-free single-shot imaging of living cells, uses low light intensities, …
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