Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 6 of 6 for “"time lapse fluorescence microscopy"”.
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Computational image analysis of subcellular dynamics in time-lapse fluorescence microscopy
… tracking algorithms was adapted for analyzing time-lapse data of cells with fluorescently labeled protein. Performance metrics were devised and algorithm parameters were matched to hand-created ground-truth data. The performance of these algorithms in this domain was compared. Finally, the …
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Quantitative analysis of axon elongation : a novel application of stochastic modeling techniques to long-term, high-resolution time-lapse microscopy of cortical axons
… for in vitro imaging of axon growth cones with time-lapse fluorescence microscopy. Image tiles are automatically captured and assembled into a mosaic image of a square millimeter region. GFP-expressing mouse cortical neurons can be imaged once every few minutes for up to weeks if phototoxicity …
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Dynamics of Cell Fate Decision Making Between Sporulation and Competence in Bacillus Subtilis
… by the transcription factor ComK. Using time-lapse fluorescence microscopy, I quantitatively measured the activities of Spo0A and ComK, along with other cross-regulatory genes, simultaneously in single B. subtilis cells. I found that, surprisingly, sporulation and competence progressed …
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The interaction of promoter chromatin architecture with the cell cycle regulates transcription activation kinetics
… promoters, we develop a method to infer real-time transcription rates from protein expression in single Saccharomyces cerevisiae cells using time-lapse fluorescence microscopy. Comparison between the instantaneous transcription rate and cell-cycle phase in each cell demonstrates the majority …
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The Effect of Topography on Surface Behavior of Pseudomonas aeruginosa
… cells at micron-scale spatial resolutions using time-lapse fluorescence microscopy, image analysis, and particle tracking techniques. We fabricated various types of micron-scale topography with curvature (particle arrays) and recti-linear features (vertical steps) and varied the critical …
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A multiplexed microfluidic platform for antibiotic susceptibility testing
… capabilities. These factors often preclude the timely administration of correct antibiotics, which complicates the clinical management of infections and exacerbates the escalating problem of antibiotic resistance development in pathogens. My work has focused on developing microfluidic platforms …