Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"thymidine kinase"”.
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Mechanisms of Cell Death Initiated in Herpes Simplex Virus Thymidine Kinase Expressing Colon Tumor Cells Treated with Ganciclovir and UCN-01
… Heterologous expression of Herpes Simplex Virus Thymidine Kinase (HSVtk) in combination with the prodrug, ganciclovir (GCV), has shown great promise for the genetic therapy of many cancers, but most patients have had only a partial or minimal response to the therapy. After screening a panel of …
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Reconstitution of a Multi-layered, Differentiated Cornea by HTERT-Immortalized Corneal Epithelial Cells Transduced with Thymidine Kinase Transplanted onto Denuded Mouse Corneas
… line (hTCEpi) transduced with the hygromycin-thymidine kinase gene (HyTK) as a viable cell source for the reconstruction of the corneal surface. METHODS: HyTK cells were cultured in KGM-2 serum-free culture media under hygromycin B selection. Limbal stem cell deficiency (LSCD) was established …
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Induzierter Zelltod von transdifferenzierten hepatischen Sternzellen (Myofibroblasten) auf der Basis des Thymidinkinase-Ganciclovir-Systems
In the current study the thymidine kinase / ganciclovir-system was used to develop a prophylaxis in oder to prevent liver fibrogenesis. The intention was to eliminate activated hepatic stellate cells (HSC), those cells which became proliferative, fibrogenetic and contractile because of liver …
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Tumor Response TCF-4/β-Catenin Regulatory Elements for Enhancing Cancer Gene Therapies
… and c-fos promoter linked with the herpes virus thymidine kinase suicide gene in combination with ganciclovir was further evaluated in the normal and tumor cell lines. The Tcf-4/β-catenin elements conferred tumor specific expression of HSV thymidine kinase (HSV-TK) gene, resulting in selective …
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Studies of the division cycle of human cancer cells (HELA) using a new system for continuous synchronization
… in HeLa cells. Cellular activity of the enzymes thymidine kinase, alkaline DNase, catalase, acid phosphatase and lactic dehydrogenase has been determined as a function of the division cycle. The method of synchronization developed here allows the continuous synchronous growth of mammalian cells …
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Directed Migration Achieved in Mammalian Cells via Ca2+ Signal Rewiring in Synthetic Protein Chimeras
… Ca2+-activated RhoA (named CaRQ), VSVG and thymidine kinase. Upon binding TNFα, TNFR1chi generates a Ca2+ signal that in turn activates CaRQ-mediated non-apoptotic blebs allowing migration towards the TNFα source. Next, the addition of VSVG, upon low pH induction, causes membrane fusion of …
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Clonage du génome l'herpesvirus cyprin-3 en tant que chromosome artificiel bactérien
… BAC flanquée de sites loxP au sein du gène de la thymidine kinase (TK). Cette insertion a généré un plasmide BAC stable en bactéries et a permis de régénérer des virions par transfection en cellules permissives. Ainsi, des virions excisés pour la cassette BAC et possédants un gène TK tronqué ont …
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Targeting The Blood-Brain Barrier With A Non-Canonical Iron-Mimicry Mechanism
… attributes enables Herpes simplex virus thymidine kinase-mediated gene therapy of intracranial tumors for molecular genetic imaging and suicide gene delivery with ganciclovir. Finally, we expand our data by analyzing a large panel of primary CNS tumors through comprehensive tissue …
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Gene Therapy for Osteosarcoma : "In Vitro" Characterization of Five Tumor Cell Lines and Development of Treatment Models "In Vivo"
… retroviral vectors containing the Herpes simplex thymidine kinase (HS-tk), human interleukin 2 (IL2). and B-galactosidase (BAG) genes. Each osteosarcoma cell line was stably transduced, and the HS-tk gene effectively conferred ganciclovir (GCV) susceptibility to transduced cells. Each cell line …
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Changes in proteins during the cell cycle of Physarum polycephalum
… in gels of nuclear proteins. Five species of thymidine kinase were identified on isoelectric focusing gels from their characteristic activities. TIP inhibited all five forms. The isoelectric points of these forms were 5.9, 6.4, 6.7, 6.9 and 7. 1. The four most acidic forms first appear about …
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Modeling of [¹⁸F]-FHBG in tumor and normal tissues
… as the substrate for the HSV-1 enzyme product - thymidine kinase (TK) - in order for PET imaging technique to identity sites of HSV-1 TK activity. The imaged mice were divided into three groups: a control group with no tumor growth and no viral injection, a control group with no tumor growth and …
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The Origin of Genome Instability in Cancer: Role of the Fragile Site Gene Product FHIT
… triphosphate pool and an insufficient supply of thymidine triphosphate. FHIT up-regulates the S-phase-specific expression of thymidine kinase 1, a component of the pyrimidine salvage pathway for the production of thymidine triphosphate. Balanced precursors of DNA are needed for efficient and …
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Uncovering the Role of 5'-Nucleotidases in Pyrimidine Metabolism in Trypanosoma brucei
… pools. Previous work in our lab discovered that thymidine kinase (TK) is necessary to balance the activity of 5'-nucleotidases. I have characterized three proteins with 5'-nucleotidase activity in T. brucei belonging to the histidine-aspartate (HD) and haloacid dehalogenase (HAD) protein …
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Identification and Characterization of Drug Targets in the Pyrimidine and Purine Pathways of Trypanosoma brucei
… essentiality two seemingly redundant enzymes: thymidine kinase (TK) and cytidine deaminase (CDA). Using a combination of genetic and analytical techniques, a novel pathway linking cytosine and thymine nucleotides was discovered. This pathway is composed of the salvage enzymes TK and CDA in …
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Enhancing Oncolytic Virotherapy Using Functional Genomic Screening
… clinical candidates that rely on deletions of thymidine kinase and/or vaccinia growth factor. We conducted RNAi VV genome-wide screens in two tumour cell lines to identify VV genes that could be deleted without diminishing its potency in tumour cells but would be concomitantly attenuating in …
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Autocrine mechanisms of action of insulin-like growth factor-I (IGF-I) and hormonal regulation of expression of IGF-finding proteins in mammary epithelial cells
… simian virus (pSV40-IGF-I), and herpes simplex thymidine kinase (pTK-IGF-I) promoters. Stably transfected clones were generated by cotransfection of clonal MAC-T cells with the IGF-I expression vectors and a plasmid conferring resistance to hygromycin-B (HYG-B), using a calcium phosphate …
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Activation of Early Neural Progenitors Is Required for Traumatic Brain Injury-Induced Hippocampal Neurogenesis
… that expresses modified herpes simplex viral thymidine kinase (delta-HSV-TK) under the control of the neural progenitor-specific regulatory element of the nestin gene. This allows for temporally regulated ablation of dividing neural progenitors by exposing the animal to ganciclovir. Using this …
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A novel approach to the clinical diagnosis and treatment of canine histiocytic sarcoma
… combined compared to when giving individually. Thymidine kinase 1 (TK1) is a marker of cellular proliferation and can be measured in the serum and used as a biomarker in both dogs and humans. In this study, we evaluated the use of TK1 along with Creactive protein in an algorithm called the …
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