Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"third-generation sequencing"”.
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A streamlined Workflow for Transposon Mutagenesis in Corynebacterium glutamicum Based on Third-Generation Sequencing and its Application for Advanced Genetic Screens
… for transposon mutant library preparation and sequencing using Oxford Nanopore Technologies (ONT) nanopore Sequencing. Mobile genetic elements (MGEs) destabilize the genetic material of their hosts. While this can contribute to evolutionary plasticity in nature, it is often detrimental in a …
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Fast and accurate alignment of barcoded reads
… we have seen the emergence of several so-called "third-generation" sequencing platforms, which improve on standard short-read sequencing that has thus far been at the center of next-generation sequencing. While technologies developed by Pacific Biosciences and Oxford Nanopore accomplish this goal …
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Exploring the genetic landscape in cutaneous T-cell lymphoma with short- and long-read sequencing
… T-cell lymphoma with short- and long-read sequencing<br /> Carsten Hain<br /> #### Abstract Cutaneous T-cell lymphoma (CTCL) are a group of diseases with heterogenous genetic alterations. In general, there are recurrent copy-number variants and rare, single nucleotide variants that …
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Improving the genome assembly and annotation of the white-tailed deer (Odocoileus virginianus borealis)
… and long single-molecule reads produced by third-generation sequencing can span these regions. I present a genome produced with DNA from a single white-tailed deer sequenced on the PacBio Sequel II platform and assembled using Redbean (WTDBG2) long-read assembly software. Post-assembly, long …
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De Novo Assembly of Genomes in Microbiomes: From Amplicons to Whole-Genome Shotgun Sequencing
In recent decades, the rapid progress of DNA sequencing technologies has greatly propelled metagenomics research. However, a critical bottleneck in current bioinformatics investigations is the assembly of metagenome at the strain-level resolution. This is indispensable for gaining profound insights …
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Tiger: tiled iterative genome assembler and approximate multi-genome aligner
… genomics. With the fast adoption of the next-generation sequencing (NGS) technologies and the coming of the third-generation sequencing (TGS) technologies, genomics has provided us with an unprecedented opportunity to answer fundamental questions in biology and elucidate human diseases. …
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Improving quality of high-throughput sequencing reads
Rapid advances in high-throughput sequencing (HTS) technologies have led to an exponential increase in the amount of sequencing data. HTS sequencing reads, however, contain far more errors than does data collected through traditional sequencing methods. Errors in HTS reads degrade the quality of …
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Next-Generation Approaches to Understanding the Diversity and Evolution of Marine Fungi
… with the increasing adoption of environmental sequencing as a primary tool for exploring fungal diversity and ecology across disparate habitats, the discovery of novel phylotypes representing new species—and in some cases, even new phyla—demands a reappraisal of fungal diversity in marine …