Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 8 of 8 for “"thiopeptides"”.
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Investigating and engineering the post-translational modifications in RiPP natural product biosynthesis (I) Thiopeptides (II) Sactipeptides
… on the thiopeptide class of RiPP antibiotics. Thiopeptides typically display nanomolar activity against pathogenic Firmicutes, such as Staphylococcus aureus, Enterococcus faecalis, and Clostridium difficile. Despite their potent activity, thiopeptides suffer from poor aqueous solubility which …
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Post-translational modifications catalyzed by RiPP and RiPP-adjacent enzymes
… on peptide scaffolds, separating pyridine-based thiopeptides from (dehydro)piperidine-based thiopeptides. This work will inform the future use of this enzyme class in engineering pyridine-based macrocycles for biotechnological and medical applications, as well as in the structural prediction of …
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Biochemical characterization of enzymes involved in the biosynthesis of the thiopeptide thiomuracin
… antibacterial agents with novel modes of action. Thiopeptides are a family of ribosomally synthesized and post-translationally modified peptides (RiPPs) that have outstanding biological profile, including high potency against a variety of antibiotic resistant pathogenic strains. Their new modes of …
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Exploring and understanding lantibiotic biosynthesis
… dehydration reaction in class I lantibiotics, thiopeptides, and goadsporin is catalyzed by LanB or LanB-like proteins. Although LanB proteins have been studied since 1992, in vitro reconstitution of their dehydration activity has been elusive. Herein, we demonstrate the in vitro activity of …
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Elucidation and control of substrate recognition during RiPP biosynthesis
… RiPP classes: linear azole-containing peptides, thiopeptides, and lasso peptides. Because numerous RiPP biosynthetic enzymes act on peptide substrates, these findings have powerful predictive value as to which protein(s) drive substrate binding, thereby laying a foundation for further …
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DNA catalysts as phosphatases and as phosphoserine lyases
… bioactive cyclic peptides such as lantibiotics, thiopeptides and microcystins. DNA enzymes were identified to establish the fundamental catalytic ability to eliminate phosphate from phosphoserine (pSer) to form Dha, namely phosphoserine lyase activity. Furthermore, DhaDz1 was utilized to achieve …
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Biochemical and structural studies on proteins involved in the production of natural products and degradation of polysaccharides
… several classes of RiPPs including cyanobactins, thiopeptides, and linear azole containing (LAP) peptides. In Chapter 2, I discuss the crystallization of the putative cyclodehydratase YcaO. My structural study of YcaO allowed for the identification and characterization of new ATP binding motif …
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Biomolecular NMR studies of the structure and biosynthesis of RiPP natural products
… compounds led to the reclassification of the thiopeptides as pyritides. In Chapter 7 genome mining approaches were used to identify and isolate a novel graspetide termed thatisin A. Results from HPLC purifications demonstrated a thermallydependent atropisomerization. Temperature-controlled NMR …