Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"thiol-reactive"”.
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Regulation of The Heat Shock Response By Thiol-Reactive Compounds In The Yeast Saccharomyces Cerevisiae
… </em>as a model system to identify a group of thiol-reactive molecules including oxidants, transition metals and metalloids, and electrophiles, as potent activators of yeast Hsf1. Using an artificial HSE-<em>lacZ </em>reporter and the glucocorticoid receptor system (GR), these diverse …
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Thiol-Based Misfolding: Linking Redox Balance to Cytosolic Proteostasis
… proteins are especially at risk as the thiol side chain is subject to oxidation, adduction and chelation by thiol-reactive compounds. All of these thiol-modifiers have been demonstrated to induce the heat shock response and recruit protein chaperones to sites of presumed protein …
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Development of luminescent probes for ultrasensitive detection of biopolymers, their complexes, and living cells
… and bi-phenyl) for the attachment of amine and thiol reactive cross-linking groups. In this project, time-resolved measurements are presented for spectroscopy and microscopy. The probes were synthesized, then characterized by UV/VIS, NMR, mass spectrometry and then validated in various …
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Development of porphyrin-antiangiogenic antibody immunoconjugates for photodynamic therapy
… monoclonal antibody (SIP(L19)). Two cationic, thiol reactive photosensitisers were synthesised and successfully conjugated to SIP(L19) in a reproducible manner, affording well defined immunoconjugates, which retained both the immunoreactivity of the antibody moiety and the photoactivity of the …
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Investigation of Prestin Membrane Topology by Substituted Cysteine Accessibility Method.
… transfected cells with a membrane impermeable thiol-reactive probe, and a fluorescent label, residues were identified as accessible (extracellular) or inaccessible (intracellular). The resulting fluorescence was quantified and analyzed. It was found that residue A170 is accessible, and that …
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The structure of a voltage gated potassium channel
… Fluorescence spectroscopy utilising cysteine reactive probes can be used to probe the membrane topology of proteins in the context of a lipid bilayer. Single cysteine mutants within the pore domain outer helix (S5 helix) of KvAP were generated and labelled with thiol reactive fluorescent …
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Peptide/Protein Stapling and Unstapling: Introduction and Photorelease of Isobutylene Graft
… most flexible approaches, as a wide range of bis-thiol-reactive linkers are commercially available. More importantly, this efficient and mild modification can be applied on large biomolecules by re-bridging their disulfides. At the same time, the handle introduced by the method provides further …
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ANALYSIS OF ELECTROPHILE-INDUCED NRF2 GENE ACTIVATION
… stress. Nrf2 concentrations are regulated by the thiol-rich sensor protein Keap1, which is an adaptor protein for Cul3-dependent ubiquitination and degradation of Nrf2. However, the links between site-specificity of Keap1 modification by electrophiles and mechanisms of Nrf2 activation are poorly …
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Spontaneous Unfolding and Refolding of FNIII Domains Assayed by Thiol Exchange
… labels FNIII domains in cell culture. We used thiol reactive DTNB to measure the kinetics of labeling of buried Cys in eleven FN III domains over a wide range of urea concentrations (0-‐‑9M). The kinetics data were globally fit using Mathematica. The results are equivalent to those of H-‐‑D …
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Elucidating the Role of Toxin-Induced Microbial Stress Responses in Biological Wastewater Treatment Process Upset
… due to shock loads of toxic electrophilic (thiol reactive) chemicals. The results indicate significant K+ efflux from the activated sludge floc structure to the bulk liquid in response to shock loads of 1-chloro-2,4-dinitrobenzene (CDNB), N-ethylmaleimide (NEM), 2,4-dinitrotoluene (DNT), …
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Interactions between Folate Deficiency and β-Nicotinamide Adenine Dinucleotide Deficiency and Conformational Study of Thymidylate Synthase and its Physiological Relevance
… Crystal structures suggested that the catalytic thiol of R163K is more susceptible to modification by oxidation compared to hTS. Thus, we postulated that the inactive conformer is more resistant to oxidation than the active conformer. To test the hypothesis, we conducted a series of studies. …
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Targeting final steps of sugar nucleotide biosynthetic pathway against <i>Aspergillus fumigatus</i>
… With regards to AfPGM, I have identified a thiol reactive compound (ISFP1) that inhibits AfPGM activity (IC50 1 μM). A crystal structure of the PGMISFP1 complex suggests that the compound inhibits the activity of AfPGM by modifying a surface cysteine (C353). ISFP1 also inhibits A. fumigatus …