Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 77 for “"thermostable"”.
-
Purification, application and immunolocalization of thermostable xylanases
Submitted in fulfillment of the requirements of the degree of Master of Technology (Biotechnology), Durban University of Technology, Durban, South Africa, 2014.
-
A Thermostable Cytochrome P450 From Sulfolobus Solfataricus
"Crystallographic studies have revealed the placement of conserved key catalytic residues and solvent in the active site that are important in the P450 dioxygen scission reaction. CYP119 is found to display a mobile F-G helix and loop region that undergoes a significant conformational change upon …
-
Two thermostable hemicellulases and their effects on wood pulps
… 80°C (10 min assay). All the cloned enzymes were thermostable with more than 95% of the enzyme activity remaining after 24 hours at 70°C, in an unbuffered solution. Buffering affected the thermostability of the enzymes. The mannanase had a half life of 48 min at 85°C. The xylanases were able to …
-
The structure, function and engineering of a thermostable nitrile hydratase
Nitrile hydratases (NHases) are enzymes that catalyse the conversion of organocyanides to amides via a non-hydrolytic hydration reaction. They are industrially relevant enzymes, currently used in the manufacture of nicotinamide and acrylamide. The target of this study belongs to the thermophilic …
-
Properties of Two Thermostable Proteases From Streptomyces Rectus Var. Proteolyticus
Made available in DSpace on 2014-12-11T18:16:52Z (GMT). No. of bitstreams: 1 7405531.pdf: 4708979 bytes, checksum: 57ef642ae623065b232857a855803c17 (MD5) Previous issue date: 1973
-
Development of Building Blocks - Thermostable Enzymes for Synthetic Pathway Biotransformation (SyPaB)
… in discovering, engineering, and producing thermostable enzymes. In this project, ribose-5-phosphate isomerase (RpiB), one of the most important enzymes in the pentose phosphate pathway, was cloned from a thermophile Thermotoga maritima, and heterologously expressed in Escherichia coli, …
-
THERMOSTABLE EXOSHELLS AS TOOLS FOR RECOMBINANT PROTEIN EXPRESSION AND IN VIVO DELIVERY
We have engineered a thermostable protein nanoparticle (tES) to improve both expression and stabilization of recombinant proteins using a single technology. tES provides steric accommodation and charge complementation to GFPuv, horseradish peroxidase (HRP), and Renilla luciferase, and Photinus …
-
Development of thermostable affinity reagents for low-cost, paper-based medical diagnostics
The timely diagnosis and treatment of disease in resource-constrained settings requires the development of robust point-of-care (POC) diagnostics, which provide accurate results and can be employed by users with minimal medical training and limited access to basic infrastructure. One of the most …
-
Improvement of engineered thermostable xylanase production via immobilization of recombinant escherichia coli onto graphene oxide
Escherichia coli is the preference host system for enzyme production by recombinant DNA technology. This was due to its promising trait that is suitable for genetically modify and the obtainability of a difference E. coli strains. But, the main downside when utilizing E. coli as a host is bacterial …
-
Characterization of a thermostable amidase and development of a bioreactor process for lactic acid production
… the biochemical properties of a newly isolated thermostable amidase from Bacillus sp. RAPc8, and development of a continuous reactor process for the production of a target product. The amidase was cloned and over-expressed in E. coli BL21 strain pNH 223 pLySs by Cameron (2001). Earlier work by …
-
Isolation and Characterization of Thermostable Alpha-Galactosidases for Application of High-Temperature Processing of Soy Molasses
… and inserted into pCR2.1 which expressed a thermostable $\alpha$-galactosidase. A 61 kDa protein was observed in partially purified extracts, similar to the deduced size of the putative gene. Both bacterial strains produce $\alpha$-galactosidases which hydrolyze soy $\alpha$-galactosides. …
-
An investigation into the biocatalytic application of the thermostable nitrile hydratase from the thermophilic strain Geobacillus pallidus RAPc8
… of 65oC and constutitively expresses a thermostable nitrile hydratase. The gene cluster containing the nitrile hydratase were cloned, sequenced, and inducibly expressed in E. coli BL21 (DE3) to levels of approximately 49 U/mg. The NHase was purified by four steps including heat …
-
Characterization of the thermostable nature of the alpha and beta tubulin proteins in Cyanidium caldarium and Cyanidioschyzon merolae
… Currently, my hypothesis concerning the thermostable tubulin expressed within these organisms is that there may be key amino acid differences in the tubulin's primary structure that confer enhanced thermostability. I am testing this hypothesis by sequencing the alpha and beta tubulin …
-
High-Yield Cellulosic Hydrogen Production by Cell-Free Synthetic Cascade Enzymes: Minimal Bacterial Cellulase Cocktail and Thermostable Polyphosphate Glucokinase
… from pretreated biomass, and preparations of thermostable enzymes with low-cost. The hydrolysis performance of 21 reconstituted bacterial cellulase mixtures containing the glycoside hydrolase family 5 Bacillus subtilis endoglucanase, family 9 Clostridium phytofermentans processive …
-
Identifying the Rubisco Recognition Domain and Critical Arginines in Activase and Engineering Activase With Improved Thermostability for Arabidopsis
… in Arabidopsis. To overcome this limitation, a thermostable activase for Arabidopsis was generated by engineering the more thermostable tobacco activase with an Arabidopsis Rubisco recognition domain. The resulting chimeric activase displayed an improved thermostability, activated Arabidopsis …
-
Molecular Dynamics Simulations Provide Insight into Stability of Hyperthermophilic Endoglucanases
… be beneficial to discover more high-efficiency, thermostable enzymes to utilize in the hydrolytic process. In this study molecular dynamics simulations were performed on structurally similar endoglucanases with varying levels of thermostability to gain insight on what factors contribute to …
-
Immunologic Characterization of Soluble Antigen Derived From Cell Culture of Babesia Bovis
… potent by inoculation of susceptible animals was thermostable, sensitive to 2-mercaptoethanol, and subject to a partial and a complete destruction by papain and alpha-amlase, respectively. Accordingly, soluble B. bovis antigen appears to be a polysacharide-protein entity or more specifically a …
-
Process integration in the optimisation of amidase production from recombinant Escherichia coli
… the investigation of the production of a novel thermostable amidase (EC 3.5.1.4) from Geobacillus pallidus RAPc8 using recombinant E.coli BL21 (DE3). The choice of growth medium and induction strategy were optimised under bioreactor conditions to enhance amidase productivity. Further, expanded …
Page 1 of 4