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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 57 for “"thermal denaturation"”.
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Studies of the Thermal Denaturation of Ribonuclease
Made available in DSpace on 2014-12-05T19:38:02Z (GMT). No. of bitstreams: 1 6206160.pdf: 4358934 bytes, checksum: 466a2edb6e041df2725196e46e996a05 (MD5) Previous issue date: 1962
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Tensile properties of ovine skeletal muscle fibres: Thermal denaturation and selective removal of titin.
… I-band contributed to the final stiffness of the thermally denatured fibres, independent of the number of cross-bridges that had formed prior to cooking. As the I-band increased in length in the raw state, the more compliant the fibres became after cooking. This thesis provides information on, and …
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ASP 53, a 53 kDa cupin-containing protein from Acacia erioloba seeds that protects proteins against thermal denaturation
Includes bibliographical references (leaves 103-111).
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Physicochemical and immunological studies of thermally induced conformational changes in protein antigens of mycobacterium bovis strain, BCG
… related to the more obvious manifestations of thermal denaturation which have been reported from studies of heated mycobacterins.
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\(Pyrococcus\) \( furiosus\) amylase as a Candidate Sterilisation Time-Temperature Integrator
Thermal treatment is the most common method used by industry to ensure food is safe for consumption and to increase storage life. To ensure safety, food is often over processed, which can significantly affect its nutritional value as well as taste and flavour attributes. In this study a candidate …
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Heat induced denaturation of fibrous hard alpha-keratins and their reaction with various chemical reagents
This dissertation is concerned with the thermal behaviour of fibrous proteins encapsulated in rigid structures, among the most well-known representatives of this class being the alpha-keratins in human hair. In spite of a lot of work in the field, there is still no mechanism proposed for accounting …
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Probing peptide binding to the second PDZ domain of hPTP1E using FT-IR and MS
… a stable protein-ligand complex had formed. Thermal denaturation of free PDZ and in combinations with three peptides was investigated using FT-IR and CD spectroscopy. The T m of free PDZ in D 2 O was found to be 47 ± 1C̕ when monitoring the loss of Ý-sheets and the appearance of aggregation …
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Understanding Allosteric Arginine Mutations Using Macromolecular Rate Theory
… has traditionally been attributed to thermal denaturation. An increasing body of evidence has shown that denaturation alone is insufficient to account for these deviations from Arrhenius behaviour. Macromolecular rate theory (MMRT) accounts for these deviations with the introduction of …
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Science and history explored by nuclear magnetic resonance
… characterize the changes that occur during the thermal denaturation of keratin fibers from wool and hair. A model describing both the effect of thermal denaturation and the effect of different chemical treatments on keratin fibers is presented. Proton NMR spectroscopy was used for studying the …
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Investigations into the role of histone H2A ubiquitination in chromatin
… nucleoprotein gel shifts, protein analyses and thermal denaturation. Ubiquitinated H2A did not inhibit linker histone binding to nucleosome cores. The effect of uH2A on nucleosome and chromatosoine positioning on a 208 bp Lytechinus variegatus 5S rDNA fragment was investigated using a …
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Part I. A Stacked Intercalator Approach to Selectively Target Mismatches Within CTG and CUG Trinucleotide Repeats. Part II. Aromatic Stacking of Naphthoyl Modified Cytosines Within PNA·DNA Duplexes
… multiple substitutions of the modified cytosine. Thermal denaturation studies and thermodynamic analyses of resulting PNA·DNA duplexes were performed to determine the stabilization due to added stacking between the naphthoyl substituents aligned within the major groove. PNA·DNA …
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Development of thermostable affinity reagents for low-cost, paper-based medical diagnostics
… and structurally complex, and are prone to thermal denaturation. In contexts where continuous cold chain storage may be infeasible, the resulting loss in binding activity can manifest as diminished assay sensitivity, leading to adverse clinical outcomes and eroding patient trust in the …
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Synthesis of aromatic peptide nucleic acids
… general structure A was synthesized in solution. Thermal denaturation studies failed to indicate if these compounds hybridize to DNA or RNA. The second generation APNA monomers ( B ) were incorporated into PNA hexamers by solid phase peptide synthesis. The resulting PNA/APNA chimeras hybridize to …
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Correlation between physical and genetic maps of plasmids
… DNA (in the form of calorimetric profiles, thermal denaturation profiles and electron micrographs of partly melted DNA sequences) and genetic maps of these DNAs and thus deal with questions which were not answered by previous researchers. viz: Is there a correlation between base sequence …
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Topologically constrained DNA intercalators: Synthesis and DNA binding studies
… using absorption spectroscopy titrations, thermal denaturation studies, and viscometric titrations of both calf thymus DNA and closed circular supercoiled DNA. Macrocycle 31 dissociates 10 to 40 times slower from poly(d(A-T)) $\sb2$ and poly(d(G-C)) $\sb2$ than its analogous mono-linked …
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The Self-Organisation of Biological Soft matter Systems at Different Length-scales
… the reversibility of unfolding following thermal denaturation of lysozyme. Solution conditions (pH, ionic strength and the presence/absence of disaccharides) were varied to systematically alter the temperature at which the protein unfolds, Tm. The enthalpies of unfolding during successive …
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Branching into RNAi: Synthesis, Characterization and Biology of Branch and Hyperbranch siRNAs
… motifs were then studied by CD spectroscopy and thermal denaturation experiments. CD spectroscopy was used to characterize the requisite A-type double-stranded RNA helix for RNAi application; whereas thermal denaturation experiments were used to validate siRNA hybrid stabilities. With stable …
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Probing the Role of Nascent Helicity in Protein Function: p27kip1 as a Regulator of the Cell Cycle
… with the Cdk2/cyclin A complex. Results from isothermal titration calorimetry (ITC) and kinase inhibition assays, show that the interaction and of either D1 or D2 with the Cdk2/cyclin A complex is less favorable relative to that of the p27-KID and that the D2 peptide cannot completely inhibit …
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Observing the unfolding transition of [beta]-hairpin peptides with nonlinear infrared spectroscopy
… secondary structure of P-hairpin peptides during thermal denaturation. Spectroscopic modeling is presented to describe the amide I band of 0-hairpins and relate it to structural features. Three spectroscopic methods are used to probe the amide I band: Fourier transform infrared (FTIR) …
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