Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 28 for “"therapeutic protein"”.
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Genomic Analysis And Engineering Of Chinese Hamster Ovary Cells For Improved Therapeutic Protein Production
Protein biologics have transformed the field of medicine in recent years. These complex molecules are produced in living cells, primarily Chinese Hamster Ovary (CHO) cells. Due to the importance of these therapeutic proteins to disease treatment, it is essential to improve the efficiency of their …
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Rheology of concentrated protein solutions and attractive colloidal dispersions
Therapeutic protein products with high solution concentration often possess extremely high viscosity and have difficulties in processing and delivery. It is desirable to predict and control the viscosity of protein solutions based on their interactions at the molecular level. Fundamental …
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Developing scalable and modular technologies for continuous biopharmaceutical production
… cold-chains, the geographically biased nature of therapeutic protein production today can limit access to biologic drugs in developing areas of the world. These same logistical hurdles can also hamper the efficient distribution of life-saving protein therapeutics following crises in developed …
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Development of preformed photo-crosslinked intravitreal implants for protein delivery to the posterior segment of the eye
… the benefits of the introduction of commercial therapeutic protein products. The treatment of disorders of the back of the eye, which are collectively referred to as 'posterior segment diseases of the eye,' is made possible by this group of proteins. These disorders include age-related macular …
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Delipidation Treatments for Large-Scale Protein Purification Processing
… insoluble. Lipid removal is desired prior to protein purification processing to decrease nonspecific fouling of downstream chromatographic matrices. Transgenic pig milk was used as a model system to study delipidation from therapeutic protein sources. The majority of triglycerides was …
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Site-specific chemical and topological modifications to augment mRNA therapeutic potential
Synthetic mRNA has emerged as a promising therapeutic platform for the treatment of a wide variety of diseases. Despite clinical demonstrations of mRNA for SARS-CoV-2 vaccines, mRNAs remain limited in application by their susceptibility to nucleases and overall short expression lifetime in vivo. We …
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The impact of variation in freezing and thawing process parameters on the critical quality attributes of a monoclonal antibody
Therapeutic proteins or biopharmaceuticals have been playing an ever-increasing role in the treatment of human diseases over last 40 years. One of the main challenges with manufacture of these proteins is the stabilization of both the finished product and its processing intermediates during …
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Engineering cell-based micropharmacies for in vivo protein replacement therapy
… disorders depends on the consistent delivery of therapeutic proteins to correct underlying deficiencies or modulate disease progression. However, conventional methods, such as enzyme replacement therapy (ERT) and cancer immunotherapies, often require frequent infusions, suffer from limited tissue …
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Cationic Polymers and Polymeric Micelles as Plasmid DNA and CRISPR-Cas9 Ribonucleoprotein Delivery Vehicles
… and disorders worldwide and the traditional protein-based treatments are both expensive and require repetitive injections to maintain long-term effects. Gene therapy, as an alternative, holds great potential by direct delivery of genetic materials such as nucleic acids and genome editing …
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Polyelectrolyte multilayer growth factor delivery : mediating tissue/device interactions
This thesis focuses on the use of ultrathin therapeutic protein delivery films to control host tissue/medical device implant interactions, thereby reducing complications that lead to implant failure. The Layer by Layer (LbL) deposition platform was used to fabricate conformal, tunable, micron scale …
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Feasibility of a predictive model of Hsp70b-activated gene therapy protein expression during ultrasound hyperthermia
… system that can control when and where the therapeutic proteins will be generated. Our study was performed to determine the feasibility of attaching heat-inducible promoters to genes of interest in order to control activation of the gene in vivo via ultrasound-induced hyperthermia monitored …
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The Engineering of Chinese Hamster Ovary Cells to Achieve More Efficient Gene Amplification for Improving Biopharmaceutical Development
… cells capable of producing high quantities of therapeutic proteins. It addresses the specific time bottlenecks associated with developing high producing CHO cell lines and reviews various methods that are employed to alleviate these bottlenecks. The specific time consuming process of gene …
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Targeting the tight junction : immunotherapy of colon cancer
A33 is a cell surface glycoprotein of colon epithelium with a long clinical history as a target in antibody-based cancer therapy. Despite being present in normal colon, radio-labeled antibodies against A33 are selectively retained by tumors at long time points. Accordingly, we have studied the …
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Evaluating future biopharmaceutical inspection needs, infrastructure capability gaps, and technology development strategies
… properties of the manufactured drug product. As therapeutic protein formulations change from agonist to antagonist methods of action, the concentration of proteins in each dose increased by orders of magnitude, and with it, the challenge of producing and inspecting the product. Current inspection …
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Receptor Mediated Oral Delivery Of Bioencapsulated Green Fluorescent Protein Expressed In Transgenic Chloroplasts
… expenses could be minimized or eliminated when therapeutic proteins are expressed and orally delivered via plant cells. Chloroplasts have the machinery to fold complex and biologically active eukaryotic proteins in the soluble chloroplast stromal compartment. Protein expression through …
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Product and host engineering for low-cost manufacturing of therapeutic proteins in the yeast Komagataella phaffii
… in low- and middle-income countries (LMICs). Therapeutic proteins, which can be stored as liquids, are more amenable to distribution in LMICs than newer modalities like therapeutic RNAs that require cryogenic storage. Currently, most therapeutic proteins are manufactured in either bacterial …
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Mass Spectrometry-Based Strategies for Biomolecular Structure Analysis: Steroid Metabolites Structures and Protein-Protein Interactions
… small molecules and large biomolecules (e.g., proteins). The majority of the applications prior to 1970 were focused on small molecules, owing to the limited ionization methods which posed difficulties in producing gas-phase ions for large biomolecules then. Beginning in the 1980's, with the …
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Recombinant Engineering Strategies for the Production of Therapeutic Coagulation Factor Proteins
… numerous blood dyscrasias and can be restored by therapeutic intervention with agents rich in coagulation factor proteins such as whole blood, fresh frozen plasma, prothrombin complex concentrates, and single coagulation factor concentrates. These agents are predominantly sourced from volunteer …
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Uniform biodegradable microparticle systems for protein delivery
Therapeutic protein drugs are receiving increasing attention in pharmaceutical industry due to the fast development of biotechnology and genomics. Because of their low oral and transdermal bioavailabilities, fragility and short half-lives in vivo, novel and effective alternatives to deliver protein …
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Making In Utero Gene Therapy Safer and More Efficient: A First Step Towards Clinical Realization
… the second delivery-vehicle, which carries the therapeutic protein and is packaged with the murine ecotropic envelope which restricts binding of the MR4IN vector to the mCAT-1 receptor, thus limiting its transduction to human or sheep cells that have been transduced by the first vector and …
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